Treatment of Relapsed or Refractory B-cell Lymphoma With Chimeric Antigen Receptor (CAR) T-cell Therapy Produced by a New Technology

NCT ID: NCT06378190

Last Updated: 2026-02-06

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

PHASE1/PHASE2

Total Enrollment

27 participants

Study Classification

INTERVENTIONAL

Study Start Date

2024-03-11

Study Completion Date

2030-07-31

Brief Summary

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The goal of this clinical trial is to to evaluate the safety and efficacy of TranspoCART19 in patients with relapsed/refractory B-lymphoma. The main questions it aims to answer are:

Maximum tolerated dose (MTD) Response rates Participants will be treated with the investigational medicinal product and will be followed for 36 months.

Detailed Description

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This clinical trial is a Phase I/II, pilot, open-label, national, prospective, multicentre, non-randomised, open-label study to evaluate the safety and efficacy of TranspoCART19 in patients with relapsed/refractory B-lymphoma whose prognosis is less than 2 years.

Phase I: Dose escalation phase with a classic 3+3 design, in which three dose levels of TranspoCART19 will be evaluated: 1 x 106 cells/kg, 3 x106 cells/kg and 5 x 106 cells/kg. The maximum number of patients included in this phase will be 18.

Phase II: an expansion cohort with the maximum tolerated dose (MTD) determined in Phase I.

Patients will be included in the expansion cohort up to a total of 27, including Phase I patients.

Conditions

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Refractory B-Cell Lymphoma B-cell Lymphoma Recurrent

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Phase I/II, pilot, open, national, prospective, multicentre, non-randomised
Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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TranspoCART19 cells

Adult differentiated, autologous, peripheral blood T lymphocytes, expanded and genetically modified.

Group Type EXPERIMENTAL

CAR-T cells therapy

Intervention Type BIOLOGICAL

CAR-T cells therapy

Interventions

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CAR-T cells therapy

CAR-T cells therapy

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

1. Patients diagnosed with relapsed or refractory B-cell lymphoma (Diffuse large B-cell lymphoma, Primary diffuse large B-cell lymphoma of the Central Nervous System (CNS), Mantle cell lymphoma, Follicular lymphoma grades 1, 2 or 3a or Marginal lymphoma, including splenic, nodal and MALT).
2. Age over 18 years and under 80 years.
3. Functional status Eastern Cooperative Oncology Group (ECOG) Performance Status 0-1. Patients with ECOG 2 may be included if motivated by haematological disease (Annex 3).
4. Adequate bone marrow haematopoietic reserve.
5. Life expectancy of at least 2 months.
6. Adequate venous access for lymphapheresis. Absence of contraindications for lymphapheresis.
7. Signed informed consent (patient or legal guardian).

Exclusion Criteria

1. Patients who, in the opinion of a physician, may benefit from other approved potentially curative therapeutic options, including commercial CAR-Ts.
2. Treatment with any experimental or non-commercialised substance in the four weeks prior to recruitment, or who are actively participating in another therapeutic clinical trial.
3. Diagnosis of another neoplasm, past or present. Patients who have been in complete remission for more than 3 years, or with a history of non-melanoma skin cancer or completely resected carcinoma in situ may be included. A current or previous history of clonal T-lymphocytes is also an exclusion criterion.
4. Early relapse after allogeneic haematopoietic stem cell transplantation (less than 3 months for lymphapheresis, less than 6 months for TranspoCART19 infusion) or patients on active immunosuppressive treatment for graft-versus-recipient disease (corticosteroids or other systemic immunosuppressants).
5. Active infection requiring systemic medical treatment.
6. HIV infection.
7. Concurrent and uncontrolled medical illnesses including cardiac, renal, hepatic, gastrointestinal, endocrine, pulmonary, neurological or psychiatric illnesses that in the opinion of the investigator pose a risk to the patient.
8. Positive serology for hepatitis B, defined as a positive test for HBsAg. In addition, if the patient is HBsAg negative but has anti-HBcore antibodies, a hepatitis B virus DNA test will be required, and if the result is positive the patient will be excluded.
9. Positive serology for hepatitis C virus (HCV), defined as a positive test for anti-HCV antibodies that is confirmed by Recombinant immunoblot assay (RIBA).
10. Severe organ involvement, defined as cardiac ejection fraction \<40%; diffusing capacity of the lungs for carbon monoxide (DLCO) \<40%; calculated glomerular filtration rate \<30 ml/min; baseline O2 saturation \<92%; bilirubin \> 2 times upper limit of normal (unless due to Gilbert's syndrome) or transaminases \> 2.5 upper limit of normal.
11. Pregnant or lactating women. Women of childbearing age should have a negative pregnancy test at screening.
12. Women of childbearing age, including those whose last menstrual cycle was in the year prior to screening, who are unable or unwilling to use highly effective methods of contraception\* from the start of the study until the end of the study.
13. Men who are unable or unwilling to use highly effective methods of contraception\* from the start of the study until the end of the study.
14. Need to take glucocorticoids chronically in doses greater than 10 mg/day of prednisone (or equivalent) or other chronic immunosuppressants.
15. Previous anti-CD19 CAR-T therapy. Previous treatment with other anti-CD19 strategies is permitted, provided that CD19 expression has been confirmed in the tumour biopsy.
16. Hypersensitivity to the active substance or to any of the excipients.
Minimum Eligible Age

18 Years

Maximum Eligible Age

80 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Spanish Clinical Research Network - SCReN

NETWORK

Sponsor Role collaborator

Fundación Canaria de Investigación Sanitaria

OTHER

Sponsor Role collaborator

Fundación para la Investigación Biomédica del Hospital 12 de Octubre

UNKNOWN

Sponsor Role collaborator

Instituto de Investigación Biomédica de Salamanca

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Hospital Clínic

Barcelona, Barcelona, Spain

Site Status NOT_YET_RECRUITING

Institut Català d'Oncologia Hospital

L'Hospitalet de Llobregat, Barcelona, Spain

Site Status RECRUITING

Fundación Jiménez Díaz Hospital

Madrid, Madrid, Spain

Site Status RECRUITING

Virgen de la Arrixaca University Hospital

El Palmar, Mur, Spain

Site Status RECRUITING

Clínica Universidad de Navarra

Pamplona, Navarre, Spain

Site Status RECRUITING

University Hospital of Navarra

Pamplona, Navarre, Spain

Site Status RECRUITING

Salamanca University Health Care Complex

Salamanca, SALAMANCA, Spain

Site Status RECRUITING

Virgen del Rocio Hospital

Seville, Sevilla, Spain

Site Status RECRUITING

Countries

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Spain

Central Contacts

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Esperanza López_Franco, PhD

Role: CONTACT

923 291200 ext. 55779

Fátima Macho Sánchez-Simón

Role: CONTACT

923 291200 ext. 55779

Facility Contacts

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Esperanza López-Franco, PhD

Role: primary

923291200 ext. 55779

Nuria Martínez Cibrian

Role: backup

Esperanza López-Franco, PhD

Role: primary

923291200 ext. 55779

Alberto Musetti

Role: backup

Esperanza López-Franco

Role: primary

923291200 ext. 55779

Javier Cornago-Navascués

Role: backup

Esperanza López-Franco, PhD

Role: primary

923291200 ext. 55779

Joaquín Gómez-Espuch

Role: backup

Esperanza López-Franco, PhD

Role: primary

923291200 ext. 55779

Carlos Grande

Role: backup

Esperanza López-Franco

Role: primary

923291200 ext. 55779

María Carmen Mateos-Rodríguez

Role: backup

Esperanza López-Franco, PhD

Role: primary

923291200 ext. 55779

Lucía López-Corral, PhD

Role: backup

Esperanza López-Franco, PhD

Role: primary

923291200 ext. 55779

José Antonio Pérez-Simón

Role: backup

References

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Diez B, Calvino C, Fernandez-Garcia M, Rodriguez-Marquez P, Rodriguez-Diaz S, Martinez-Turillas R, Ceballos C, Illarramendi J, Serrano-Lopez J, Miskey C, Navarro-Bailon A, Lopez-Corral L, Llamas P, Redondo M, Sanchez-Guijo F, Rifon J, Alfonso-Pierola A, Ivics Z, Inoges S, Lopez-Diaz de Cerio A, Yanez R, Bueren JA, Rodriguez-Madoz JR, Prosper F. Generation and GMP scale-up of human CAR-T cells using non-viral Sleeping Beauty transposons for B cell malignances. Mol Ther Methods Clin Dev. 2025 Jan 31;33(1):101425. doi: 10.1016/j.omtm.2025.101425. eCollection 2025 Mar 13.

Reference Type DERIVED
PMID: 40034423 (View on PubMed)

Other Identifiers

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2022-001040-23

Identifier Type: EUDRACT_NUMBER

Identifier Source: secondary_id

2024-514544-90-00

Identifier Type: CTIS

Identifier Source: secondary_id

TranspoCART19

Identifier Type: -

Identifier Source: org_study_id

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