Study of Autologous CAR-T Cells Targeting B7-H3 in TNBC iC9-CAR.B7-H3 T Cells

NCT ID: NCT06347068

Last Updated: 2025-09-16

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

PHASE1

Total Enrollment

42 participants

Study Classification

INTERVENTIONAL

Study Start Date

2024-06-27

Study Completion Date

2028-05-31

Brief Summary

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This phase 1, single-center, open-label study explores the safety of escalating doses of chimeric antigen receptor T cells (CAR-T) cells in subjects with relapsed/refractory triple-negative breast cancer (TNBC).

Detailed Description

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T lymphocyte chimeric antigen receptor cells against the B7-H3 antigen (iC9-CAR.B7-H3 T cells) treatment is experimental and has not been approved by the Food and Drug Administration. The safety of iC9-CAR.B7-H3 T cells will be investigated using a modified 3+3 design. The data from the dose escalation will be used to determine a recommended phase 2 dose (RP2D), which will be decided based on the maximum tolerated dose (MTD) and additional factors such as the ability to manufacture sufficient cells for infusion.

Subjects with TNBC who meet procurement eligibility criteria will have cells collected to manufacture iC9-CAR.B7-H3 T cells. Eligible subjects will receive lymphodepletion with cyclophosphamide and fludarabine.

Conditions

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Breast Cancer Relapse Resistant Cancer Triple Negative Breast Cancer

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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iC9-CAR.B7-H3 T cells

Specimen will be collected to prepare the iC9-CAR.B7-H3 T cells. Disease-fighting T cells will be isolated and modified to prepare the iC9-CAR.B7-H3 T cells. In part 2, the iC9-CAR.B7-H3 T cells are given by infusion after completion of lymphodepletion chemotherapy.

Group Type EXPERIMENTAL

iC9-CAR.B7-H3 T Cell Therapy

Intervention Type BIOLOGICAL

iC9-CAR.B7-H3 T cells will then be administered intravenously

cyclophosphamide

Intervention Type DRUG

cyclophosphamide 300 mg/m2 IV will be given.

fludarabine

Intervention Type DRUG

fludarabine 30 mg/m2 IV will be given.

Interventions

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iC9-CAR.B7-H3 T Cell Therapy

iC9-CAR.B7-H3 T cells will then be administered intravenously

Intervention Type BIOLOGICAL

cyclophosphamide

cyclophosphamide 300 mg/m2 IV will be given.

Intervention Type DRUG

fludarabine

fludarabine 30 mg/m2 IV will be given.

Intervention Type DRUG

Other Intervention Names

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iC9-CAR.B7-H3 T cells Cytoxan Fludara Fludarabine Phosphate

Eligibility Criteria

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Inclusion Criteria

Unless otherwise noted, subjects must meet all of the following criteria to participate in in all phases of the study:

1. Written informed consent and Health Insurance Portability and Accountability Act (HIPAA) authorization for release of personal health information explained to, understood by and signed by the subject or legally authorized representative.
2. Age ≥ 18 years at the time of consent.
3. Karnofsky score of \> 60% (see APPENDIX VI- Karnofsky Scale))
4. Histologically confirmed TNBC (ER-, PR-, HER2-negative)

1. ER- and PR-negative: defined as \< 1% staining by immunohistochemistry (IHC)
2. HER2-negative: defined as IHC 0-1+ or fluorescence in situ hybridization (FISH) ratio \< 2.0

Exclusion Criteria

1. Patients with a history of symptomatic CNS involvement or multiple metastases requiring whole-brain radiation.
2. Subjects with a prior or concurrent malignancy whose natural history or treatment has the potential to interfere with the safety or efficacy assessment of the investigational regimen are eligible for this trial.
3. Subject does not have a measurable and or evaluable disease as defined by RECIST 1.1
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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National Cancer Institute (NCI)

NIH

Sponsor Role collaborator

M.D. Anderson Cancer Center

OTHER

Sponsor Role collaborator

UNC Lineberger Comprehensive Cancer Center

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Yara E Abdou, MD

Role: PRINCIPAL_INVESTIGATOR

UNC Lineberger Comprehensive Cancer Center

Locations

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University of North Carolina

Chapel Hill, North Carolina, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Caroline Babinec

Role: CONTACT

919-445-4208

Catherine Cheng

Role: CONTACT

+1 919-445-4208

Facility Contacts

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Catherine Cheng

Role: primary

919-445-4208

Related Links

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http://unclineberger.org/patientcare/clinical-trials/clinical-trials

University of North Carolina Lineberger Comprehensive Cancer Center Clinical Trials

Other Identifiers

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P50CA058223-29A1

Identifier Type: NIH

Identifier Source: secondary_id

View Link

LCCC2128-ATL

Identifier Type: -

Identifier Source: org_study_id

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