Volatolomic and Proteomic Profile for Early Diagnosis of Lung Cancer

NCT ID: NCT06341387

Last Updated: 2024-04-02

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

200 participants

Study Classification

OBSERVATIONAL

Study Start Date

2023-05-05

Study Completion Date

2024-07-31

Brief Summary

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The goal of this prospective, case-control study is to discover the specific "omics" biomarkers of early stage of lung cancer using the non-invasive samples (breath, urine and serum) in a total of 200 subjects (100 healthy controls and 100 lung cancer patient). The main questions it aims to answer are:

* Which are the "omics" biomarkers that characterize the early stage of lung cancer?
* How to Translate Laboratory Data into Clinical Data?

For each participant we will collected the breath, urine and blood samples. In lung cancer patients group the samples will be sample before lung cancer resection. The samples of Breath, urine and serum will be analysed using different type of analysis: eNose and the Gas Chromatography combined with Ion Mass Spectrometry (GC/IMS). Moreover, Serum will be analyzed by mass-spectrometry-based proteomics. The purpose of these analyses will be to find biomarkers capable of distinguishing the early-stage of lung cancer from the healthy group. Followup will be performed to evaluate the possible change of the volatolomic and proteomic profile.

Detailed Description

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All partecipants will sign the Informed Consent before the sampling procedures. In addition, they will complete the clinical questionnaire containing medical history, smoking history and psychological evaluation.We will conduct the trial according to the ICH Good Clinical Practice (GCP) guidelines. Keeping accurate and consistent records is essential to a cooperative study.The IEO Data Management Office will responsible of the study database and data management.

Conditions

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Lung Cancer Non-small Cell Lung Cancer Stage I Non-small Cell Lung Cancer Stage II Lung Cancer Diagnosis

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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Lung cancer patients

Age 50-80 years Diagnosis of early stage of lung cancer No previous chemo or radiotherapy for lung cancer No previous malignancies within last 5 years No abuse of alcohol (no more than 1 litre of wine for day). No patients with psychiatric, addictive, or any disorder, which compromises ability to give informed consent for participation in this study Signed Informed Consent Completed questionnaire

Breath, urine and blood analysis

Intervention Type OTHER

breath sampling: all subjects exhale into two sterile Tedlar bags, connected to a mouthpiece, in a condition similar to traditional spirometry.

Urine will be collected usual urine container.

The blood sample (\~ 5 ml) will be taken with a serum separator tube.

High Risk-Healthy Subjects

Age 50-80 years high risk individuals (heavy smokers, subjects with pulmonary disease non-cancer related or with a familiar history of lung cancer disease) Recent (within 6 months) negative Chest X-ray or CT scan No previous malignancies within last 5 years No patients with psychiatric, addictive, or any disorder, which compromises ability to give informed consent for participation in this study Signed Informed Consent Completed questionnaire

Breath, urine and blood analysis

Intervention Type OTHER

breath sampling: all subjects exhale into two sterile Tedlar bags, connected to a mouthpiece, in a condition similar to traditional spirometry.

Urine will be collected usual urine container.

The blood sample (\~ 5 ml) will be taken with a serum separator tube.

Interventions

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Breath, urine and blood analysis

breath sampling: all subjects exhale into two sterile Tedlar bags, connected to a mouthpiece, in a condition similar to traditional spirometry.

Urine will be collected usual urine container.

The blood sample (\~ 5 ml) will be taken with a serum separator tube.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

1. Lung cancer group

* Diagnosis of early stage - lung cancer
* Signed Informed Consent
* Completed questionnaire
2. Healthy subjects

* high risk individuals (heavy smokers, subjects with pulmonary disease non-cancer related or with a familiar history of lung cancer disease)
* Recent (within 6 months) negative Chest X-ray or CT scan

Exclusion Criteria

Both groups

* No previous chemo or radiotherapy for lung cancer
* No previous malignancies within last 5 years
* No abuse of alcohol (no more than 1 litre of wine for day).
* No patients with psychiatric, addictive, or any disorder, which compromises ability to give informed consent for participation in this study
Minimum Eligible Age

50 Years

Maximum Eligible Age

80 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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University of Rome Tor Vergata

OTHER

Sponsor Role collaborator

European Institute of Oncology

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Europen insitute of Oncology- Division of Thoracic Surgery

Milan, , Italy

Site Status RECRUITING

Countries

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Italy

Central Contacts

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Roberto Gasparri, MD, PhD

Role: CONTACT

0257489499

Facility Contacts

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Roberto Gasparri, MD, PhD

Role: primary

0257489499

Other Identifiers

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R178522-ieo1906

Identifier Type: -

Identifier Source: org_study_id

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