A Clinical Study of CD19 CAR NK Cells for the Treatment of Relapsed/Refractory B-cell Related Autoimmune Diseases

NCT ID: NCT06318533

Last Updated: 2025-05-11

Basic Information

• Recruitment Status: RECRUITING
• Clinical Phase: EARLY_PHASE1
• Total Enrollment: 15 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2024-03-13
• Study Completion Date: 2027-03-13

Brief Summary

A single arm, open-label pilot study is designed to determine the safety and effectiveness of anti-CD19 CAR NK cells (KN5501) in patients with relapsed/refractory B-cell related autoimmune diseases.15 patients are planned to be enrolled in the dose-escalation trial (6×10^9 cells, 9×10^9 cells). The primary objective of the study is to evaluation of the safety and feasibility of KN5501 for the treatment of relapsed/refractory B-cell related autoimmune diseases. The secondary objective is to evaluate the effectiveness of KN5501 for the treatment of relapsed/refractory B-cell related autoimmune diseases. The exploratory objective is to evaluate expansion, persistence and ability to deplete CD19 positive B cells of KN5501 in patients with relapsed/refractory B-cell related autoimmune diseases.

Conditions

Autoimmune Diseases

Study Design

• Allocation Method: NA
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: TREATMENT
• Blinding Strategy: NONE

Study Groups

anti-CD19 CAR NK cells

Group Type: EXPERIMENTAL

anti-CD19 CAR NK cells

Intervention Type: DRUG

Patients will receive Fludarabine (30mg/m2 per day) and Cyclophosphamide (300mg/m2 per day) on day -5, -4, and -3, followed by Anti-CD19 CAR NK cells infusion.

Interventions

anti-CD19 CAR NK cells

Patients will receive Fludarabine (30mg/m2 per day) and Cyclophosphamide (300mg/m2 per day) on day -5, -4, and -3, followed by Anti-CD19 CAR NK cells infusion.

Intervention Type: DRUG

Eligibility Criteria

Inclusion Criteria

1. Subjects voluntarily participate in this clinical study and sign the Informed Consent Form (ICF) and are willing to follow and be able to complete all trial procedures

2. Subjects disease status of enrolment: not complete response (CR) after standard treatment; moderately to severely active autoimmune diseases

3. Age: ≥ 18 years old and ≤ 70 years old, male or female

4. Subjects with estimated survival > 12 weeks

5. Adequate organs function: Serum creatinine clearance meets relevant age/sex criteria,aspartate aminotransferase (AST) and alanine aminotransferase (ALT) ≤ 3 times the upper limit of normal (ULN)

6. ECOG performance ≤ 2

7. Left ventricular ejection fraction (LVEF) ≥ 45%

8. Subjects have been treated with OCS in combination with an immunosuppressive or biologic agent for at least 2 weeks prior to enrollment

Exclusion Criteria

1. Subjects with known severe allergic reactions, hypersensitivity, contraindication to any medications during the trial (cyclophosphamide, fludarabine, tozumabs), or subjects with a history of severe allergic reactions

2. Subjects with one of the following genetic syndromes: Fanconi syndrome, Kostmann syndrome, Shwachman syndrome or any of the known bone marrow failure syndromes

3. Subjects with active or uncontrolled infections requiring parenteral antimicrobials; evidence of severe active viral or bacterial infections or uncontrolled systemic fungal infections

4. Subjects with grade III or IV heart failure (NYHA classification)

5. History of epilepsy or other central nervous system (CNS) diseases

6. History of other primary malignant tumors except: cured non-melanoma skin cancer or primary cervical cancer; subjects with inactive tumors

7. Subjects with more pronounced bleeding tendencies, such as gastrointestinal bleeding, coagulation disorders, and hypersplenism

8. Subjects were treated with systemic corticosteroids concomitantly within 2 weeks prior to treatment

9. Subjects with unstable angina, symptomatic congestive heart failure or myocardial infarction within the last 6 months

10. Females who are pregnant, lactating, or planning a pregnancy within six months

11. Subjects who have received other clinical trial treatment within 3 months

12. Any situation judged by the investigators that may increase the risk of the subjects or interfere with the clinical trial outcome

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 70 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Rui Therapeutics Co., Ltd

INDUSTRY

Sponsor Role: collaborator

YANRU WANG

OTHER

Sponsor Role: lead

Responsible Party

YANRU WANG

Director, Department of Rheumatology and Immunology

Responsibility Role: SPONSOR_INVESTIGATOR

Locations

Affiliated Hospital of Jiangsu University

Zhenjiang, Jiangsu, China

Site Status: RECRUITING

Jiangsu University Affiliated Hospital

Zhenjiang, Jiangsu, China

Site Status: RECRUITING

Countries

China

Facility Contacts

Yu Tang

Role: primary

tangtang@ujs.edu

086-13815153350

Yanru Wang

Role: primary

tangtang@ujs.edu

0511-85026079

Other Identifiers

2023-11-01

Identifier Type: -

Identifier Source: org_study_id