Colorectal Omics and OfCS Proteoglycans

NCT ID: NCT06287671

Last Updated: 2024-12-30

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

1000 participants

Study Classification

OBSERVATIONAL

Study Start Date

2024-10-08

Study Completion Date

2032-12-31

Brief Summary

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This observational study aims to test proteomics, metabolomics and proteoglycans as predictors of postoperative complications after colorectal surgery and as biomarkers of colorectal cancer.

The main questions to answer are:

* can these biomarkers predict anastomotic leakages
* can these biomarkers predict recurrence after colorectal cancer
* can these biomarkers be used as diagnostic tests for colorectal cancer
* can these biomarkers be identified in the tumor

Participants will undergo elective colorectal resection or stoma closure.

Detailed Description

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The prospective cohort study will include 1,000 patients undergoing elective colorectal resections or colostomy reversal at two colorectal centers in Denmark. Repeating study blood samples will be collected on each postoperative day (POD) 1-4 or until discharge. If the participants are diagnosed with cancer, blood sampling is planned 26-35 days after the index procedure, and after one, two, and three years, a tumor biopsy will be taken from the fresh specimen in the operation theatre.

Analyses of blood plasma and tissue for oncofetal chondroitin sulfate (ofCS) proteoglycans, proteomics, and metabolomics will be performed on those repeating blood samplings to:

* To investigate whether metabolomics, proteomics, and ofCS techniques can identify new biomarkers where charges in plasma levels can predict or detect subclinical AL (primary outcome) and other major postoperative complications after colorectal surgery and prediction of 90-day and three-year mortality.
* To investigate whether the APOE genotype is associated with the risk of AL and other major postoperative complications and long-term outcomes, i.e., recurrence and mortality, after colorectal surgery.
* To examine whether metabolomics and proteomics can identify new biomarkers predicting recurrence after colorectal cancer resections.
* To identify other potential biomarkers that might enable early cancer diagnosis.
* Whether proteoglycans can be used as a diagnostic test with a high degree of separability to identify tumor markers that are usable in a clinical setting (primary outcome). Participants with colorectal cancer will be compared with a control group of participants with benign conditions.
* Whether the level of proteoglycans measured correlates to the tumor load.
* Whether the proteoglycans and proteomics detected in plasma are presented in tumor tissue from the resected specimen.
* Whether the proteoglycans detected can be used as tumor markers with a high degree or measure of separability for monitoring recurrence after colorectal cancer in a clinical setting (primary outcome).

Postoperative and follow-up data will be collected prospectively for the electronic health records.

Conditions

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Colorectal Cancer Diverticulitis, Colonic Inflammatory Bowel Diseases Stoma Colostomy

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Study cohort

Patients undergoing elective colorectal resection or stoma closure

Colorectal resection or stoma closure

Intervention Type PROCEDURE

Elective open or minimally invasive resection for colorectal cancer, IBD or other benign colorectal disorder, or stoma closure

Interventions

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Colorectal resection or stoma closure

Elective open or minimally invasive resection for colorectal cancer, IBD or other benign colorectal disorder, or stoma closure

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

* Patients diagnosed or with suspicion of colorectal cancer or adenoma, inflammatory bowel disease, late complications to colon diverticulosis, colostomy reversal or other diagnoses requiring colorectal resection.
* Patients planned to undergo elective surgical procedures coded as KJFB20-KJFB99, KJFG30-37 or KJGB00-97 according to the Danish modification of the NOMESCO Classification of Surgical Procedures
* Able to speak Danish, English, or other languages where professional interpretation is available
* Able to give informed consent

Exclusion Criteria

* Patients undergoing synchronous: liver resection (patients undergoing metastasectomies can be included); total gastrectomy or cardia resection; Whipple's procedure or another major pancreatic resection (resections of the pancreatic tail can be included); total or partial nephrectomies or cystectomy
* Patients previously included in the study
* Patients known to be pregnant (pregnancy test not required)
* Non-resident in Denmark
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Viborg Regional Hospital

OTHER

Sponsor Role collaborator

Claus Anders Bertelsen, PhD, MD

OTHER

Sponsor Role lead

Responsible Party

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Claus Anders Bertelsen, PhD, MD

Clinical research professor, consultant colorectal surgeon

Responsibility Role SPONSOR_INVESTIGATOR

Principal Investigators

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Claus A Bertelsen, PhD

Role: STUDY_CHAIR

Department of Surgery, Copenhagen University Hospital - North Zealand

Locations

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Nordsjaellands Hospital

Hillerød, , Denmark

Site Status RECRUITING

Regionshospitalet Viborg

Viborg, , Denmark

Site Status NOT_YET_RECRUITING

Countries

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Denmark

Central Contacts

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Nanna B Hupfeld, ph.d.

Role: CONTACT

Phone: +4548292793

Email: [email protected]

Facility Contacts

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Nanna B Hupfeld, MD

Role: primary

Claus A Bertelsen, PhD

Role: backup

Anders S Tøttrup, PhD

Role: primary

Other Identifiers

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COCO

Identifier Type: -

Identifier Source: org_study_id