The Effects of Antibiotic Exposure in Early Infancy on Vaccine Responses

NCT ID: NCT06079606

Last Updated: 2025-12-18

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

ACTIVE_NOT_RECRUITING

Total Enrollment

200 participants

Study Classification

OBSERVATIONAL

Study Start Date

2022-09-10

Study Completion Date

2027-12-31

Brief Summary

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This prospective cohort study will include 200 term children born in Landspitali University Hospital in Iceland. The cohort will be divided into four groups according to antibiotic exposure of which one is the control group. Children's immune responses to live oral rotavirus vaccine as well as conventional vaccinations given at three, five and 12 months old will be determined by measuring salivary and serologic responses. Responses will be compared between the four groups. Stool samples will also be collected from participants and the composition of the microbiome compared between the four groups.

Detailed Description

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This prospective cohort study will evaluate the development of the immune system by means of evaluating the immune responses to oral rotavirus vaccine and the scheduled childhood vaccinations in Iceland, at the age of three, five and 12 months, in four different groups of term children born in Iceland.

The cohort will be divided into the following groups:

* Group A: Term children born with elective caesarean section (their mothers received intra-operative antibiotics).
* Group B: Term vaginally born children and their mothers received intra-partum antibiotics.
* Group C: Term vaginally born children that received systemic antibiotics during the first week of life for at least 48 hours.
* Group D: Term vaginally born children that had not received systemic antibiotics at the time of recruitment.

Parents of children born with elective caesarean section in Landspitali or born vaginally and their mothers received intra-partum antibiotics as well as parents of children in the Neonatal Intensive Care Unit receiving systemic antibiotics during the first week of life will be offered to take part in the study (Groups A, B and C). Parents of children coming to a five day old check up at the Children's Hospital in Landspitali will also be offered to take part in the study (Group D). Sample size calculations show that to detect a 30% difference in immune responses to vaccines with 80% power, 40 infants are needed for analysis in each group. To account for late exclusions due to antibiotic use or drop-out due to other reasons, 50 infants will be recruited to each group. Participants will receive vaccination with the oral live attenuated rotavirus vaccine, Rotarix, at six weeks old and again at three months old. Participants will receive vaccinations with Pentavac against diphtheria, tetanus, pertussis, haemophilus influenza type B and polio and Synflorix against pneumococci at their Health Care Centre according to scheduled childhood vaccinations in Iceland at three, five and 12 months old. Salivary and blood samples will be taken from participants just before the first vaccination with Rotarix at six weeks old and again at six months (four weeks after the second dose of Pentavac and Synflorix) and at 13 months old (four weeks after the third dose of Pentavac and Synflorix). Specific antibody levels against rotavirus, pertussis, diphtheria, tetanus, haemophilus influenzae type B, polio virus and pneumococci will be measured. Parents of participants will be given two stool sample kits and asked to take one from their child at six weeks old (before the first dose of rotavirus vaccine) and then another at three months old. In case of detection of differences in antibody response, these samples will be sent for microbiome analysis.

Conditions

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Immune Responses Antibiotic Treatment

Keywords

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Antibiotics Immune Responses Vaccinations Children Rotavirus

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Group A

Term children born with caesarean section (their mothers received intra-operative antibiotics)

Antibiotic treatment

Intervention Type OTHER

Children will be assigned to groups according to antibiotic exposure in early life. No antibiotics will be given in the study.

Group B

Term vaginally born children and their mothers received intra-partum antibiotics

Antibiotic treatment

Intervention Type OTHER

Children will be assigned to groups according to antibiotic exposure in early life. No antibiotics will be given in the study.

Group C

Term vaginally born children that received systemic antibiotics during the first week of life for at least 48 hours

Antibiotic treatment

Intervention Type OTHER

Children will be assigned to groups according to antibiotic exposure in early life. No antibiotics will be given in the study.

Group D (Control group)

Term vaginally born children that had not received systemic antibiotics at the time of recruitment

No interventions assigned to this group

Interventions

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Antibiotic treatment

Children will be assigned to groups according to antibiotic exposure in early life. No antibiotics will be given in the study.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* All term children born in Iceland from 2022-2023

Exclusion Criteria

* Children born with emergency caesarean section
* Children born before 36 weeks of gestation
* Children diagnosed with primary or secondary immunodeficiency
* Use of immunosuppressive drugs at recruitment or during the follow up period (excluding inhaled corticosteroids and short term oral corticosteroids (\<3 consecutive days).
* Children with chromosomal, genetic or other congenital diseases or abnormalities
* Children with severe, chronic diseases or disabilities
* Informed consent not signed by parents
Maximum Eligible Age

6 Weeks

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Landspitali University Hospital

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Landspitali University Hospital

Reykjavik, , Iceland

Site Status

Countries

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Iceland

Other Identifiers

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e-INFANT-2

Identifier Type: -

Identifier Source: org_study_id