Trial Outcomes & Findings for Immunogenicity of RSV Vaccines in Residents of Long-Term Care Facilities (LTCF) (NCT NCT06077149)

NCT ID: NCT06077149

Last Updated: 2025-10-27

Results Overview

Microneutralization Assay (MNA): Serum neutralizing titers will be performed using an established microneutralization method for RSV A and B strains. Briefly, 2-fold serum dilutions are incubated with 75 plaque forming units of RSV for 30 min at room temperature followed by the addition of 104HEp-2cells in 96-well culture plates. After 3 days, the quantity of RSV antigen is determined by enzyme immunoassay using a monoclonal antibody to the RSV Fa protein. The geometric mean fold rise is the neutralizing antibody titer at day 30 divided by the neutralizing antibody titer at day 0. Calculated as the geometric mean of the fold rise (post-vaccination titer divided by baseline titer) across participants.

Recruitment status

COMPLETED

Study phase

PHASE4

Target enrollment

152 participants

Primary outcome timeframe

30 days

Results posted on

2025-10-27

Participant Flow

Participant milestones

Participant milestones
Measure
Long-term care facility residents
Licensed RSV vaccine IM x 1. Specific product to be determined availability at the facility as SOC RSV vaccine: All participants will get either the Pfizer RSV vaccine (RSVpreF (ABRYSVO)) or the GSK RSV vaccine (RSVpreF (AREXVY)) at 120 ug of the RSV prefusion F antigen intramuscularly in the the non-dominant deltoid muscle. Long-term care facility residents will get whichever vaccine is available at the facility as part of standard of care. Community cohort will be matched proportionally
community dwelling adults
Licensed RSV vaccine IM x1. The proportion of specific products will be matched to the LTCF cohort RSV vaccine: All participants will get either the Pfizer RSV vaccine (RSVpreF (ABRYSVO)) or the GSK RSV vaccine (RSVpreF (AREXVY)) at 120 ug of the RSV prefusion F antigen intramuscularly in the the non-dominant deltoid muscle. Long-term care facility residents will get whichever vaccine is available at the facility as part of standard of care. Community cohort will be matched proportionally
Overall Study
STARTED
76
76
Overall Study
COMPLETED
70
76
Overall Study
NOT COMPLETED
6
0

Reasons for withdrawal

Reasons for withdrawal
Measure
Long-term care facility residents
Licensed RSV vaccine IM x 1. Specific product to be determined availability at the facility as SOC RSV vaccine: All participants will get either the Pfizer RSV vaccine (RSVpreF (ABRYSVO)) or the GSK RSV vaccine (RSVpreF (AREXVY)) at 120 ug of the RSV prefusion F antigen intramuscularly in the the non-dominant deltoid muscle. Long-term care facility residents will get whichever vaccine is available at the facility as part of standard of care. Community cohort will be matched proportionally
community dwelling adults
Licensed RSV vaccine IM x1. The proportion of specific products will be matched to the LTCF cohort RSV vaccine: All participants will get either the Pfizer RSV vaccine (RSVpreF (ABRYSVO)) or the GSK RSV vaccine (RSVpreF (AREXVY)) at 120 ug of the RSV prefusion F antigen intramuscularly in the the non-dominant deltoid muscle. Long-term care facility residents will get whichever vaccine is available at the facility as part of standard of care. Community cohort will be matched proportionally
Overall Study
Death
1
0
Overall Study
Withdrawal by Subject
2
0
Overall Study
RSV infection before vaccination
3
0

Baseline Characteristics

Immunogenicity of RSV Vaccines in Residents of Long-Term Care Facilities (LTCF)

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Long-term care facility residents
n=76 Participants
Licensed RSV vaccine IM x 1. Specific product to be determined availability at the facility as SOC RSV vaccine: All participants will get either the Pfizer RSV vaccine (RSVpreF (ABRYSVO)) or the GSK RSV vaccine (RSVpreF (AREXVY)) at 120 ug of the RSV prefusion F antigen intramuscularly in the the non-dominant deltoid muscle. Long-term care facility residents will get whichever vaccine is available at the facility as part of standard of care. Community cohort will be matched proportionally
community dwelling adults
n=76 Participants
Licensed RSV vaccine IM x1. The proportion of specific products will be matched to the LTCF cohort RSV vaccine: All participants will get either the Pfizer RSV vaccine (RSVpreF (ABRYSVO)) or the GSK RSV vaccine (RSVpreF (AREXVY)) at 120 ug of the RSV prefusion F antigen intramuscularly in the the non-dominant deltoid muscle. Long-term care facility residents will get whichever vaccine is available at the facility as part of standard of care. Community cohort will be matched proportionally
Total
n=152 Participants
Total of all reporting groups
Age, Continuous
79.6 years
STANDARD_DEVIATION 9.6 • n=5 Participants
72.6 years
STANDARD_DEVIATION 6.3 • n=7 Participants
75.9 years
STANDARD_DEVIATION 8.9 • n=5 Participants
Sex: Female, Male
Female
47 Participants
n=5 Participants
45 Participants
n=7 Participants
92 Participants
n=5 Participants
Sex: Female, Male
Male
29 Participants
n=5 Participants
31 Participants
n=7 Participants
60 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
4 Participants
n=5 Participants
1 Participants
n=7 Participants
5 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
72 Participants
n=5 Participants
75 Participants
n=7 Participants
147 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Asian
0 Participants
n=5 Participants
4 Participants
n=7 Participants
4 Participants
n=5 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Black or African American
7 Participants
n=5 Participants
0 Participants
n=7 Participants
7 Participants
n=5 Participants
Race (NIH/OMB)
White
69 Participants
n=5 Participants
72 Participants
n=7 Participants
141 Participants
n=5 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Region of Enrollment
United States
76 participants
n=5 Participants
76 participants
n=7 Participants
152 participants
n=5 Participants
Number of participants with diabetes mellitus
25 Participants
n=5 Participants
16 Participants
n=7 Participants
41 Participants
n=5 Participants
Number of participants with coronary artery disease
28 Participants
n=5 Participants
6 Participants
n=7 Participants
34 Participants
n=5 Participants
Number of participants with congestive heart failure
20 Participants
n=5 Participants
2 Participants
n=7 Participants
22 Participants
n=5 Participants
Number of participants with obstructive pulmonary disease
5 Participants
n=5 Participants
14 Participants
n=7 Participants
19 Participants
n=5 Participants
Number of participants with Asthma
5 Participants
n=5 Participants
5 Participants
n=7 Participants
10 Participants
n=5 Participants
Number of participants with hypertension
61 Participants
n=5 Participants
27 Participants
n=7 Participants
88 Participants
n=5 Participants
Number of participants with chronic kidney disease
24 Participants
n=5 Participants
2 Participants
n=7 Participants
26 Participants
n=5 Participants
Number of participants with cerebrovascular accident
24 Participants
n=5 Participants
0 Participants
n=7 Participants
24 Participants
n=5 Participants
Number of participants with dementia
33 Participants
n=5 Participants
0 Participants
n=7 Participants
33 Participants
n=5 Participants
Number of participants with cancer
15 Participants
n=5 Participants
4 Participants
n=7 Participants
19 Participants
n=5 Participants
Mean number of medical conditions
5.6 number of medical conditions
STANDARD_DEVIATION 1.8 • n=5 Participants
2.2 number of medical conditions
STANDARD_DEVIATION 1.8 • n=7 Participants
3.9 number of medical conditions
STANDARD_DEVIATION 2.5 • n=5 Participants

PRIMARY outcome

Timeframe: 30 days

Microneutralization Assay (MNA): Serum neutralizing titers will be performed using an established microneutralization method for RSV A and B strains. Briefly, 2-fold serum dilutions are incubated with 75 plaque forming units of RSV for 30 min at room temperature followed by the addition of 104HEp-2cells in 96-well culture plates. After 3 days, the quantity of RSV antigen is determined by enzyme immunoassay using a monoclonal antibody to the RSV Fa protein. The geometric mean fold rise is the neutralizing antibody titer at day 30 divided by the neutralizing antibody titer at day 0. Calculated as the geometric mean of the fold rise (post-vaccination titer divided by baseline titer) across participants.

Outcome measures

Outcome measures
Measure
Long-term care facility residents
n=70 Participants
Licensed RSV vaccine IM x 1. Specific product to be determined availability at the facility as SOC RSV vaccine: All participants will get either the Pfizer RSV vaccine (RSVpreF (ABRYSVO)) or the GSK RSV vaccine (RSVpreF (AREXVY)) at 120 ug of the RSV prefusion F antigen intramuscularly in the the non-dominant deltoid muscle. Long-term care facility residents will get whichever vaccine is available at the facility as part of standard of care. Community cohort will be matched proportionally
community dwelling adults
n=76 Participants
Licensed RSV vaccine IM x1. The proportion of specific products will be matched to the LTCF cohort RSV vaccine: All participants will get either the Pfizer RSV vaccine (RSVpreF (ABRYSVO)) or the GSK RSV vaccine (RSVpreF (AREXVY)) at 120 ug of the RSV prefusion F antigen intramuscularly in the the non-dominant deltoid muscle. Long-term care facility residents will get whichever vaccine is available at the facility as part of standard of care. Community cohort will be matched proportionally
Geometric Mean Fold Rise Against RSV Fa Protein
12.5 fold change
Interval 12.0 to 12.9
9.9 fold change
Interval 9.8 to 9.9

PRIMARY outcome

Timeframe: 30 days

Microneutralization Assay (MNA): Serum neutralizing titers will be performed using an established microneutralization method for RSV A and B strains. Briefly, 2-fold serum dilutions are incubated with 75 plaque forming units of RSV for 30 min at room temperature followed by the addition of 104HEp-2cells in 96-well culture plates. After 3 days, the quantity of RSV antigen is determined by enzyme immunoassay using a monoclonal antibody to the RSV Fb protein. The geometric mean fold rise is the neutralizing antibody titer at day 30 divided by the neutralizing antibody titer at day 0. Calculated as the geometric mean of the fold rise (post-vaccination titer divided by baseline titer) across participants.

Outcome measures

Outcome measures
Measure
Long-term care facility residents
n=70 Participants
Licensed RSV vaccine IM x 1. Specific product to be determined availability at the facility as SOC RSV vaccine: All participants will get either the Pfizer RSV vaccine (RSVpreF (ABRYSVO)) or the GSK RSV vaccine (RSVpreF (AREXVY)) at 120 ug of the RSV prefusion F antigen intramuscularly in the the non-dominant deltoid muscle. Long-term care facility residents will get whichever vaccine is available at the facility as part of standard of care. Community cohort will be matched proportionally
community dwelling adults
n=76 Participants
Licensed RSV vaccine IM x1. The proportion of specific products will be matched to the LTCF cohort RSV vaccine: All participants will get either the Pfizer RSV vaccine (RSVpreF (ABRYSVO)) or the GSK RSV vaccine (RSVpreF (AREXVY)) at 120 ug of the RSV prefusion F antigen intramuscularly in the the non-dominant deltoid muscle. Long-term care facility residents will get whichever vaccine is available at the facility as part of standard of care. Community cohort will be matched proportionally
Geometric Mean Fold Rise Against RSV Fb Protein
11.0 fold change
Interval 10.5 to 11.6
8.7 fold change
Interval 8.6 to 8.8

PRIMARY outcome

Timeframe: 30 days

Microneutralization Assay (MNA): Serum neutralizing titers will be performed using an established microneutralization method for RSV A and B strains. Briefly, 2-fold serum dilutions are incubated with 75 plaque forming units of RSV for 30 min at room temperature followed by the addition of 104HEp-2cells in 96-well culture plates. After 3 days, the quantity of RSV antigen is determined by enzyme immunoassay using a monoclonal antibody to the RSV A2 protein. The geometric mean fold rise is the neutralizing antibody titer at day 30 divided by the neutralizing antibody titer at day 0. Calculated as the geometric mean of the fold rise (post-vaccination titer divided by baseline titer) across participants.

Outcome measures

Outcome measures
Measure
Long-term care facility residents
n=70 Participants
Licensed RSV vaccine IM x 1. Specific product to be determined availability at the facility as SOC RSV vaccine: All participants will get either the Pfizer RSV vaccine (RSVpreF (ABRYSVO)) or the GSK RSV vaccine (RSVpreF (AREXVY)) at 120 ug of the RSV prefusion F antigen intramuscularly in the the non-dominant deltoid muscle. Long-term care facility residents will get whichever vaccine is available at the facility as part of standard of care. Community cohort will be matched proportionally
community dwelling adults
n=76 Participants
Licensed RSV vaccine IM x1. The proportion of specific products will be matched to the LTCF cohort RSV vaccine: All participants will get either the Pfizer RSV vaccine (RSVpreF (ABRYSVO)) or the GSK RSV vaccine (RSVpreF (AREXVY)) at 120 ug of the RSV prefusion F antigen intramuscularly in the the non-dominant deltoid muscle. Long-term care facility residents will get whichever vaccine is available at the facility as part of standard of care. Community cohort will be matched proportionally
Geometric Mean Fold Rise Against RSV A2 Protein
15.5 fold change
Interval 15.2 to 16.0
12.8 fold change
Interval 12.6 to 12.9

SECONDARY outcome

Timeframe: 30 days

the ratio of fold response of RSV A2 neutralizing titer to Fa IgG binding titers was calculated as a measure of the relative proportion of functional F antibody produced

Outcome measures

Outcome measures
Measure
Long-term care facility residents
n=70 Participants
Licensed RSV vaccine IM x 1. Specific product to be determined availability at the facility as SOC RSV vaccine: All participants will get either the Pfizer RSV vaccine (RSVpreF (ABRYSVO)) or the GSK RSV vaccine (RSVpreF (AREXVY)) at 120 ug of the RSV prefusion F antigen intramuscularly in the the non-dominant deltoid muscle. Long-term care facility residents will get whichever vaccine is available at the facility as part of standard of care. Community cohort will be matched proportionally
community dwelling adults
n=76 Participants
Licensed RSV vaccine IM x1. The proportion of specific products will be matched to the LTCF cohort RSV vaccine: All participants will get either the Pfizer RSV vaccine (RSVpreF (ABRYSVO)) or the GSK RSV vaccine (RSVpreF (AREXVY)) at 120 ug of the RSV prefusion F antigen intramuscularly in the the non-dominant deltoid muscle. Long-term care facility residents will get whichever vaccine is available at the facility as part of standard of care. Community cohort will be matched proportionally
Ratio of Fold Response of RSV A2 Titer to Fa Titer
1.15 proportion of A2 to Fa
Interval 1.0 to 1.32
1.18 proportion of A2 to Fa
Interval 1.07 to 1.29

Adverse Events

Long-term care facility residents

Serious events: 0 serious events
Other events: 0 other events
Deaths: 1 deaths

community dwelling adults

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Ann Falsey, MD

University of Rochester

Phone: 585-922-4339

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place