A Study to Evaluate the Efficacy of Amniotic Suspension Allograft in Patients With Osteoarthritis of the Knee

NCT ID: NCT06000410

Last Updated: 2025-12-23

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE3

Total Enrollment

594 participants

Study Classification

INTERVENTIONAL

Study Start Date

2023-09-06

Study Completion Date

2025-11-30

Brief Summary

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This study is being conducted to evaluate the efficacy and safety of ASA compared to placebo in the management of osteoarthritis (OA) symptoms of the knee.

Detailed Description

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This is a prospective, multicenter, randomized, double-blind, placebo-controlled Phase 3 study of ASA in patients with OA of the knee. Initially, 474 subjects are planned for inclusion in this study using a group sequential design with an interim analyses and a final analysis. Patients will be randomly assigned in a 1:1 ratio to receive a single intra-articular (IA) injection of 2 mL of ASA (plus 2 mL of normal saline) or 4 mL of normal saline on Day 1.

They will have serial assessments of knee pain, function, and symptoms scores, as well as safety assessments for up to 52 weeks after administration of the study drug.

Conditions

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Knee Osteoarthritis

Keywords

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Osteoarthritis Knee Arthritis Joint Disease Musculoskeletal Diseases Rheumatic Diseases

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

This is a prospective, multicenter, randomized, double-blind, placebo-controlled study of ASA in patients with OA of the knee. Patients will be randomly assigned in a 1:1 ratio to receive a single IA injection of 2 mL of ASA (plus 2 mL of normal saline) or 4 mL of normal saline.
Primary Study Purpose

TREATMENT

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors
Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor) All clinic staff who may be involved in making assessments of safety will be blinded to treatment assignment.

Study Groups

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ASA

Participants receive a single IA injection of 2 mL of ASA (plus 2 mL of normal saline)

Group Type EXPERIMENTAL

Amniotic Suspension Allograft

Intervention Type BIOLOGICAL

This investigational product is a cryopreserved product derived from human amnion and cells from the amniotic fluid.

Placebo

Participants receive a single IA injection of 4 mL of normal saline

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type DRUG

Matching placebo is 0.9% normal saline: 4 mL to be injected IA.

Interventions

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Amniotic Suspension Allograft

This investigational product is a cryopreserved product derived from human amnion and cells from the amniotic fluid.

Intervention Type BIOLOGICAL

Placebo

Matching placebo is 0.9% normal saline: 4 mL to be injected IA.

Intervention Type DRUG

Eligibility Criteria

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Inclusion Criteria

* Males or females 18 years of age or older
* Diagnosis of OA of the index knee by a combination of clinical and radiographic findings.
* OA of the index knee with Kellgren and Lawrence radiographic classification (Grades 2-4 inclusive),
* Patients who have failed to adequately respond for at least 6 months to at least two osteoarthritis therapies within the last 12 months
* Overall index knee pain score above minimum required WOMAC Pain scale.
* Body mass index \< 40 kg/m2
* Using birth control, sterile or post-menopausal.
* Able to understand and provide written informed consent

Exclusion Criteria

* Kellgren and Lawrence radiographic grade 1 OA of the knee
* Use of pain medication less than 5 days before the baseline visit
* Regular use of anticoagulants
* Symptoms that could indicate meniscal displacement or an IA loose body.
* Corticosteroid injection into the index knee within 3 months prior to screening.
* Viscosupplement or any other autologous or allogeneic product into the index knee within 6 months prior to screening.
* Patients with known hypersensitivity reactions to ASA or any of its constituents.
* Knee surgery on the index knee within 12 months prior to screening and/or planned knee surgery during the study
* Knee surgery on the contralateral knee within 6 months prior to screening and/or planned knee surgery during the study
* Acute index knee trauma within 3 months prior to screening
* Knee effusion requiring aspiration of the index or contralateral knee within 3 months prior to screening.
* Contralateral knee pain above limits defined in the protocol
* Current therapy with any immunosuppressive therapy or medical conditions likely to require systemic steroids during the study.
* Any active or systemic infection including infection of the index knee joint or breakdown or disease of the index knee skin/soft tissues.
* Clinically significant intercurrent illness, medical condition, non-knee pain, or medical history that would jeopardize patient safety, limit participation, or compromise interpretation of data derived from the patient
* Active alcohol or substance use disorder, or any other reason that would make it unlikely that the patient will comply with study procedures
* Females who are pregnant or lactating
* Participation in another clinical trial within the 30 days (or 5 half-lives of the investigational compound, whichever is longer) before screening.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Organogenesis

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Central Research Associates, Inc.

Birmingham, Alabama, United States

Site Status

Alabama Clinical Therapeutics, LLC

Birmingham, Alabama, United States

Site Status

Fiel Family & Sports Medicine

Tempe, Arizona, United States

Site Status

Horizon Clinical Research

La Mesa, California, United States

Site Status

Neurovations Research

Napa, California, United States

Site Status

Infinity Clinical Research

Norco, California, United States

Site Status

Dream Team Clinical Research

Pomona, California, United States

Site Status

Stanford Medicine

Redwood City, California, United States

Site Status

TriWest Research Associates

San Diego, California, United States

Site Status

AppleMed Research Group, LLC.

Miami, Florida, United States

Site Status

Gulfcoast Research Institute

Sarasota, Florida, United States

Site Status

CenExcel/ACMR

Atlanta, Georgia, United States

Site Status

Vista Clinical Research

Newnan, Georgia, United States

Site Status

Chicago Clinical Research Institute Inc.

Chicago, Illinois, United States

Site Status

Rush University Medical Center

Chicago, Illinois, United States

Site Status

Advanced Quality Medical Research, Inc

Orland Park, Illinois, United States

Site Status

MediSphere Medical Research Center

Evansville, Indiana, United States

Site Status

Sinai Hospital

Baltimore, Maryland, United States

Site Status

Klein & Associates M.D., P.A.

Hagerstown, Maryland, United States

Site Status

Skylight Health Research

Burlington, Massachusetts, United States

Site Status

Oakland Medical Research

Troy, Michigan, United States

Site Status

Sundance Clinical Research

St Louis, Missouri, United States

Site Status

Physician Research Collaboriation, LLC

Lincoln, Nebraska, United States

Site Status

Quality Clinical Research

Omaha, Nebraska, United States

Site Status

NYU Langone Health

New York, New York, United States

Site Status

Hospital for Special Surgery

New York, New York, United States

Site Status

Northwell Health

New York, New York, United States

Site Status

Upstate Clinical Research Associates, LLC

Williamsville, New York, United States

Site Status

West Clinical Research

Morehead City, North Carolina, United States

Site Status

The Ohio State University Wexner Medical Center

Columbus, Ohio, United States

Site Status

Conrad Clinical Research

Edmond, Oklahoma, United States

Site Status

Tekton Research

Yukon, Oklahoma, United States

Site Status

Lehigh Center for Clinical Research

Allentown, Pennsylvania, United States

Site Status

Clinical Trials of South Carolina

Charleston, South Carolina, United States

Site Status

Tekton Research

Austin, Texas, United States

Site Status

Cedar Health Research, LLC

Burleson, Texas, United States

Site Status

ClinRXResearch INC

Carrollton, Texas, United States

Site Status

Zenos Clinical Research

Dallas, Texas, United States

Site Status

JBR Clinical Research

Salt Lake City, Utah, United States

Site Status

Spectrum Medical, Inc.

Danville, Virginia, United States

Site Status

Spokane Joint Replacement Center

Spokane, Washington, United States

Site Status

Countries

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United States

Other Identifiers

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22 OA 003 ASA

Identifier Type: -

Identifier Source: org_study_id