The Uninfected Ixodes Scapularis Human Tick Challenge Model

NCT ID: NCT05965635

Last Updated: 2023-07-28

Basic Information

• Recruitment Status: UNKNOWN
• Clinical Phase: NA
• Total Enrollment: 7 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2023-07-31
• Study Completion Date: 2024-07-31

Brief Summary

An innovative way to prevent multiple tick-borne diseases is an anti-tick vaccine, i.e. targeting the vector to prevent transmission of pathogens from the tick to the host. The rationale for an anti-tick vaccine stems from a phenomenon coined tick immunity. This study aims to show proof of concept that humans indeed develop immunity to ticks. Therefore subjects will be challenged three to four times with ticks reared in colony from a designated laboratory and that are exhaustively tested negative for various known tick-borne pathogens. Several tick feeding parameters will be evaluated to assess the development of tick immunity in the subjects. To this end, a total amount of ten ticks per challenge will be placed under a dressing placed on the forearm. The primary study endpoint is the tick feeding phenotype. Secondary parameters are signs of an immune response in the host; itch, redness, or other signs of a (local) immune response (blood and skin biopsies). All parameters, except for the skin biopsies, will be collected/evaluated after each tick challenge.

Detailed Description

This will be an, single centre, experimental study. The design of the study is similar to a human challenge trial, with the important exception that the subjects in this study are not experimentally infected with a pathogen, but are exposed to well-characterized laboratory-reared uninfected nymphal Ixodes scapularis ticks.

Seven healthy human volunteers of >18 years old who have no history of known tick-bites or tick-borne diseases will be recruited. Subjects will undergo three to four tick challenges, consisting of five to six days per challenge with two weeks between the consecutive challenges. The total duration of participation is approximately five months for each subject. By making use of ticks from a laboratory-reared tick colony that have been, and are being, used in xenodiagnosis and tick challenge models in the USA and that are well tolerated minimizing the risks of the tick challenges. Regardless, the tick challenge can cause some discomfort because of possible local reactions and because the dressing must be kept dry for five to six days. The risk of infection with a tick-borne pathogen is negligible due to the fact that these ticks are exhaustively tested for various known tick-borne pathogens. Finally, risks are considerated of development of a red meat allergy or other allergic reactions negligible because no clear associations are known of I. scapularis ticks and red meat allergy. No serious adverse events (such as human infections or red meat allergy) have resulted from more than >100 challenges in the United States using this tick colony. There are no direct benefits of participation.

Conditions

Tick Bites; Tick Immunity

Study Design

• Allocation Method: NA
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: BASIC_SCIENCE
• Blinding Strategy: NONE

Study Groups

Experimental

Group Type: EXPERIMENTAL

Ixodus scapularis nymphs

Intervention Type: OTHER

Tick challenge

Interventions

Ixodus scapularis nymphs

Tick challenge

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

• Age: 18 years and older;
• Negative Borrelia VlsE1/pepC10 ELISA.

Exclusion Criteria

• Known history of tick bites;
• Positive Borrelia serology (VlsE1/PepC10 ELISA);
• Known history or current suspicion on any tick-borne disease;
• Chronic skin condition affecting the arm skin;
• Inability to maintain the dressing for any reason;
• Known immunodeficiency or autoimmune disease;
• Use of systemic immunomodulating drugs or chemotherapy, current or in the past;
• Chronic use of antibiotics;
• Unable to give informed consent or do not have a thorough command of the Dutch language;
• Refusal to participate in specimen collection and storage for future study related use;
• Pregnant or breastfeeding women;
• Not willing to use adequate contraception during the study period;
• Red meat allergy (both medically confirmed and self-reported);
• Use of investigational therapy and devices during the time of the study;
• Any other condition that, in the opinion of the investigator, would make the patient unsuitable for enrolment or could interfere with the patient participating in and completing the study.

• Minimum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

ZonMw: The Netherlands Organisation for Health Research and Development

OTHER

Sponsor Role: collaborator

Academisch Medisch Centrum - Universiteit van Amsterdam (AMC-UvA)

OTHER

Sponsor Role: lead

Responsible Party

prof. J.W.R. Hovius, MD, PhD

PI

Responsibility Role: PRINCIPAL_INVESTIGATOR

Locations

AUMC location AMC

Amsterdam-Zuidoost, North Holland, Netherlands

Site Status: RECRUITING

Countries

Netherlands

Central Contacts

Hannelore. JL Beaart, PhD

Role: CONTACT

tickme@amsterdamumc.nl

+31205667906

Stefanie A Gauw, Coordinator

Role: CONTACT

tickme@amsterdamumc.nl

+31205667906

Facility Contacts

H. Beaart, Msc

Role: primary

TICKME@amsterdamumc.nl

0031207327170

Other Identifiers

NL81259.018.22

Identifier Type: -

Identifier Source: org_study_id