Versatile Ampification Single-Molecule Detection in Liquid Biopsy

NCT ID: NCT05940311

Last Updated: 2024-11-27

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

20 participants

Study Classification

OBSERVATIONAL

Study Start Date

2022-04-27

Study Completion Date

2026-03-31

Brief Summary

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The trial will test a paradigm-changing in vitro diagnostic device for Liquid Biopsy enabling facile simultaneous detection of protein and nucleic acid analytes with sensitivity at single-molecule level, e.g. not achievable with any alternative technology. A novel affinity-mediated transport amplification (AMT) method will be tested allowing for the multiplexed quantification of rare biomarkers circulating in blood. The Versilib AMT photonic biosensor will test two analytes: the known actionable DNA mutation BRAF p.V600E, and a melanoma-restricted protein antigen. The results will be compared to digital PCR and ELISA methods.

Detailed Description

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Conditions

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Liquid Biopsy Melanoma (Skin) Melanoma Stage III Melanoma Stage IV

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Interventions

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Blood sampling in addition to the blood normally required for their clinical management

Patients will undergo blood sampling in addition to the blood normally required for their clinical management. The study aims to collect 50 blood samples (one to 4) from 20 patients undergoing treatment in the adjuvant or advanced (metastatic) setting, as per standard of care. The first blood sample (T1) will be carried out immediately before the treatment, the subsequent blood samples (T2-T4) will coincide with the periodic radiographic re-evaluations, typically at months 3 and 6 (M3 and M6, T2 and T3), and at progression/recurrence, if and when recorded.

Intervention Type PROCEDURE

Blood plasma and circulating tumor DNA (ctDNA)

Blood drawing by venepuncture (elbow) in K2EDTA vacutainers to obtain blood plasma.The patients will otherwise receive the most appropriate treatment for their condition. The following data will be collected and pseudo-anonymised: demographic data (age and gender), histopathology including primary and metastatic sites, BRAF status, medical imaging, previous therapies assigned if any

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

* age: ≥ 18
* PFS≤2
* Patients willing to sign an informed consent;
* Confirmed (cytologically or histologically) cutaneous melanoma diagnosis
* Confirmed BRAF p. V600E tumor status
* Eligible for BRAFi/MEKi treatment or Immune checkpoint blockade in either the adjuvant or advanced settings (the latter typically stages III/IV, high risk).

Exclusion Criteria

* Life expectancy \<8 weeks
* Other clinical conditions preventing blood drawing compliance, as per physician's choice.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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VTT Technical Research Centre, Finland

OTHER

Sponsor Role collaborator

Institute of Health Information and Statistics of the Czech Republic

OTHER_GOV

Sponsor Role collaborator

Universita degli Studi di Catania

OTHER

Sponsor Role collaborator

FINNADVANCE (FIN)

UNKNOWN

Sponsor Role collaborator

PDC Biotech GmbH

INDUSTRY

Sponsor Role collaborator

Regina Elena Cancer Institute

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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"Regina Elena" National Cancer Institute

Rome, , Italy

Site Status RECRUITING

Countries

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Italy

Central Contacts

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Elena Giordani, PhD

Role: CONTACT

+39 06 5266 2533

Patrizio Giacomini, Doctor

Role: CONTACT

+39 0652665054

Facility Contacts

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Elena Giordani, PhD

Role: primary

+39 06 5266 2533

Patrizio Giacomini, Doctor

Role: backup

Related Links

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Other Identifiers

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RS1685/22 (2670)

Identifier Type: -

Identifier Source: org_study_id