The Effect of Combination Therapy of Oral MB and PRP-FG in Patients With Non-healing Diabetic Foot Ulcer

NCT ID: NCT05850611

Last Updated: 2023-10-04

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

EARLY_PHASE1

Total Enrollment

20 participants

Study Classification

INTERVENTIONAL

Study Start Date

2023-04-30

Study Completion Date

2024-09-21

Brief Summary

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The aim of this study is to evaluate the chance of non-healing diabetic foot ulcers repair by improving the condition of lack of oxygen or hypoxia in the wound area caused by diabetes using methylene blue along with the use of platelet-rich plasma-fibrin glue as an effective treatment for wound healing.

Detailed Description

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The current study assesses the effects of methylene blue along with the use of platelet-rich plasma-fibrin glue on wound healing in patients with nonhealing diabetic foot ulcers (non-healing DFU). This randomized controlled trial is performed on 20 patients with non-healing DFU. Patients were treated with PRP-FG dressing plus 200 ml of oral methylene blue dissolved in milk(intervention group) or PRP-FG dressing plus placebo (200 ml of milk) (control group) for 4 weeks.

Conditions

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Diabetic Foot Ulcers Diabetic Wound

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

FACTORIAL

Primary Study Purpose

TREATMENT

Blinding Strategy

DOUBLE

Participants Outcome Assessors

Study Groups

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Methylene Blue

The first intervention group (group A) includes diabetic patients with chronic foot ulcers who, despite common treatments, will be undergone oral methylene blue intervention (70 mg in 200 ml of milk) for 4 weeks.

Group Type EXPERIMENTAL

Methylene Blue

Intervention Type DRUG

The first intervention group includes diabetic patients with chronic foot ulcers who, despite common treatments, will be undergone oral methylene blue intervention for 4 weeks.

Milk (control)

Patients with non-healing Diabetic Foot Ulcers only will receive 200 ml of milk for 4 weeks.

Group Type EXPERIMENTAL

Milk (control)

Intervention Type OTHER

Group B will receive 200 ml of milk for 4 weeks.

Methylene Blue and Platelet-Rich Plasma-Fibrin Glue

Patients with non-healing Diabetic Foot Ulcers will treat with PRP-Fibrin-Glue plus oral methylene blue (70 mg in 200 ml of milk) for 4 weeks.

Group Type EXPERIMENTAL

Methylene Blue and Platelet-Rich Plasma-Fibrin Glue

Intervention Type DRUG

Group C will be undergone synergistic 4-week treatment with oral methylene blue and fibrin glue.

Milk and Platelet-Rich Plasma-Fibrin Glue (control)

Patients with non-healing Diabetic Foot Ulcers will be treated with PRP-Fibrin-Glue and 200 ml of milk for 4 weeks.

Group Type EXPERIMENTAL

Milk and Platelet-Rich Plasma-Fibrin Glue (control)

Intervention Type OTHER

Group D will be treated only with fibrin glue and 200 ml of milk for 4 weeks.

Interventions

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Methylene Blue

The first intervention group includes diabetic patients with chronic foot ulcers who, despite common treatments, will be undergone oral methylene blue intervention for 4 weeks.

Intervention Type DRUG

Milk (control)

Group B will receive 200 ml of milk for 4 weeks.

Intervention Type OTHER

Methylene Blue and Platelet-Rich Plasma-Fibrin Glue

Group C will be undergone synergistic 4-week treatment with oral methylene blue and fibrin glue.

Intervention Type DRUG

Milk and Platelet-Rich Plasma-Fibrin Glue (control)

Group D will be treated only with fibrin glue and 200 ml of milk for 4 weeks.

Intervention Type OTHER

Other Intervention Names

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Methylthioninium chloride Placebo Methylthioninium chloride and PRP-Fibrin Glue Placebo

Eligibility Criteria

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Inclusion Criteria

* Having a Diabetic ulcer grade II and IV based on Wagner's classification on the sole, medial, or lateral part of the foot (including all surfaces of the toes)
* Having a single ulcer on the feet and extremities (toes, soles, heels) with no significant reduction in ulcer size (\<20%) despite the use of best treatment methods for at least four weeks
* If there is more than one non-healing wound, choose the largest wound
* The size of the wound surface (length × width) between 2 cm2 and 20 cm2
* No smoking, alcohol, and drug addiction based on the patient's self-report
* Not taking drugs that may interfere with wound healing, such as Corticosteroids, immunosuppressants, and cytotoxic agents
* Not having a concurrent chronic disease that may cause problems in wound healing, such as cancers, vasculitis, no history of known severe kidney, liver, and heart disease, such as liver cirrhosis, active hepatitis, dialysis, etc.
* Not taking antidepressants
* Insensitivity to milk lactose
* Not having Glucose 6-phosphate dehydrogenase (G6PD) deficiency
* Confirmed, informed, signed consent form
* Ankle Brachial Index (ABI) higher than or equal to 0.7

Exclusion Criteria

* Do not be treated with methylene blue
* The confirmed presence of osteomyelitis, or if there is suspicion of osteomyelitis
* The subject is pregnant or intends to become pregnant during the test period
* The patient is known to have mental, developmental, physical, and emotional disorders
* The occurrence of certain medical conditions
* The presence of a wound with a clear and severe infection, which is characterized by significant purulent secretions or extensive cellulitis, or gangrene requiring amputation
* Evidence of venous, ischemic, neurotrophic ulcers (numbness, tingling, lack of Achilles tendon reflex) and traumatic wounds in the patient
* Failure to refer the patient more than two times to the mentioned center for follow-up and dressing change
* Hypersensitivity reaction to methylene blue
* Platelet count less than 100,000
* The patient's lack of consent to continue cooperation
Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Mashhad University of Medical Sciences

OTHER

Sponsor Role lead

Responsible Party

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Daryoush Hamidi Alamdari, PhD

Associate professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Daryoush Hamidi Alamdari, Ph.D

Role: PRINCIPAL_INVESTIGATOR

Mashhad University of Medical Sciences, Mashhad, Iran

Locations

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Mashhad University of Medical Sciences

Mashhad, Razavi Khorasan Province, Iran

Site Status RECRUITING

Countries

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Iran

Central Contacts

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Daryoush Hamidi Alamdari, Ph.D

Role: CONTACT

+98 51 3882 8574

Elaheh Emadi, Ph.D

Role: CONTACT

+98 9355850931

Facility Contacts

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Daryoush Hamidi Alamdari, Ph.D

Role: primary

98 51 3882 8574

Elaheh Emadi, Ph.D

Role: backup

98 9355850931

Other Identifiers

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IRCT20191228045924N3

Identifier Type: -

Identifier Source: org_study_id

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