Autophagy in Paediatric Crohn's Disease

NCT ID: NCT05842564

Last Updated: 2024-12-12

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

NOT_YET_RECRUITING

Clinical Phase

NA

Total Enrollment

70 participants

Study Classification

INTERVENTIONAL

Study Start Date

2025-01-31

Study Completion Date

2027-04-30

Brief Summary

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Crohn's disease is a multifactorial complex disease resulting in a between microbiota and immune system. Indeed, GWAS (Genome-Wide Association Studies) association study pinpointed polymorphisms as genes susceptibility on more than 200 loci. Among them genes coding for proteins involved in autophagy machinery (i.e: ATG16L1, IRGM et NDP52). Autophagy is a ubiquitous intracellular mechanism mandatory for protein and microorganism recycling.

So far, the role of autophagy in gut inflammation and intestinal homeostasis in Crohn's disease patients is partially understand. Then, investigators plan to evaluate, on native cells, the autophagic flux in pediatric patients suffering of a Crohn's disease compare to controls.

Detailed Description

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Conditions

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Crohn Disease Pediatric Crohns Disease

Keywords

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Crohn's Disease Autophagy Polymorphism

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

SINGLE_GROUP

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

SINGLE

Participants

Study Groups

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Crohn's Disease Group

Patients followed for a Crohn's Disease in consultation or in day hospital aged between 6 and 18 years old.

Group Type EXPERIMENTAL

Blood samples

Intervention Type OTHER

Blood sample of maximum 20ml (5 tubes of EDTA of 4ml or 5 tubes of EDTA of 2ml and a tube for the conservation of genomic DNA)

Biopsies

Intervention Type OTHER

5 biopsies (one for each segment of the intestine explored: ileum, right colon, transverse colon left colon and sigmoid) will be taken during the ileocolonoscopy

Control Group

Patients followed for functional abdominal disorders

Group Type OTHER

Blood samples

Intervention Type OTHER

Blood sample of maximum 20ml (5 tubes of EDTA of 4ml or 5 tubes of EDTA of 2ml and a tube for the conservation of genomic DNA)

Biopsies

Intervention Type OTHER

5 biopsies (one for each segment of the intestine explored: ileum, right colon, transverse colon left colon and sigmoid) will be taken during the ileocolonoscopy

Interventions

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Blood samples

Blood sample of maximum 20ml (5 tubes of EDTA of 4ml or 5 tubes of EDTA of 2ml and a tube for the conservation of genomic DNA)

Intervention Type OTHER

Biopsies

5 biopsies (one for each segment of the intestine explored: ileum, right colon, transverse colon left colon and sigmoid) will be taken during the ileocolonoscopy

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

For Crohn's Disease group :

* Age between 6 and 17 inclusive
* Patients with a weight \> 25 kg at the time of inclusion
* Patients with Crohn's disease requiring ileocolonoscopy (diagnosis or follow-up)
* Mild to severe Crohn's disease consistent with PCDAI disease activity score
* Patients on nutritional therapy (Modulen/Modulife), corticosteroids, salicylic derivatives, immunosuppressants, biotherapies (anti-TNF, vedolizumab and ustekinumab) or without treatment
* Consent form signed by the patient or the holder(s) of parental authority.
* Affiliation to a social security scheme or beneficiaries of a similar scheme.

For Control group:

* Between 6 and 17 years old included
* Presenting a weight \> 25 kg at the time of inclusion
* Without a diagnosis of Crohn's disease
* Requiring evaluation by ileoendoscopy
* Consent form signed by the patient or the holder(s) of parental authority.
* Affiliation to a social security scheme or beneficiaries of a similar scheme.

Exclusion Criteria

* Refusal to participate in the protocol
* Intercurrent infection
* Ongoing antibiotic treatment
* Patient involved in another interventional study protocol including an exclusion period still in progress at pre-inclusion
* Pregnant, parturient or breastfeeding women (on questioning)
* Persons deprived of their liberty by a judicial or administrative decision
* Persons subject to psychiatric care
* Persons admitted to a health or social establishment for purposes other than research
Minimum Eligible Age

6 Years

Maximum Eligible Age

17 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Hospices Civils de Lyon

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Service Hépatologie, Gastroentérologie et Nutrition pédiatrique, Hôpital Femme Mère Enfant, HCL

Bron, , France

Site Status

Countries

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France

Central Contacts

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Rémi DUCLAUX-LORAS, MD, PhD

Role: CONTACT

Phone: 0472357050

Email: [email protected]

Facility Contacts

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Rémi DUCLAUX-LORAS, MD, PhD

Role: primary

Other Identifiers

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69HCL21_1020

Identifier Type: -

Identifier Source: org_study_id