Study of LYL314 in Aggressive Large B-Cell Lymphoma

NCT ID: NCT05826535

Last Updated: 2025-12-24

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

PHASE1/PHASE2

Total Enrollment

270 participants

Study Classification

INTERVENTIONAL

Study Start Date

2023-05-09

Study Completion Date

2031-06-30

Brief Summary

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This is a Phase 1/2, multi-center, open-label study evaluating the safety and efficacy of LYL314, a dual-targeting chimeric antigen receptor (CAR) targeting cluster of differentiation (CD)19 and CD20 in participants with aggressive large B-cell lymphoma.

Detailed Description

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This is a Phase 1/2, multi-center, open-label study evaluating the safety and efficacy of LYL314, a dual-targeting chimeric antigen receptor (CAR) targeting cluster of differentiation (CD)19 and CD20 in participants with aggressive large B-cell lymphoma.

Five cohorts of participants will be enrolled:

Cohort 1: Participants who have not received a prior CAR T-cell product (CAR T naïve) and have received at least two or more prior lines of treatment (third-line or later).

Cohort 2: Participants who have received a prior CAR T-cell product (CAR T experienced) and have received at least two or more prior lines of treatment including one CAR T-cell therapy.

Cohort 3: Participants with refractory disease or relapse within one year of first-line therapy (second-line).

Cohort 4: Participants who have received prior T-cell engager (TCE) therapy and have received at least two or more prior lines of treatment including one TCE therapy.

Cohort 5: Participants receiving first-line treatment for high-risk large B-cell lymphoma who remain with disease on positron emission tomography scanning (PET-positive) after 2 to 3 cycles of standard-of-care chemoimmunotherapy (high-risk first-line).

Up to approximately 150 participants (across all cohorts) will be enrolled in the dose finding Phase 1 part of the study.

The Phase 2 pivotal study (PiNACLE) will expand enrollment of Cohort 1 to approximately 120 participants to further evaluate the safety and efficacy of LYL314.

LYL314 treatment consists of a single administration of CAR transduced autologous T-cells administered intravenously after a conditioning chemotherapy regimen consisting of fludarabine and cyclophosphamide, administered over 3 days.

Individual participants will remain in the active post-treatment follow-up (PTFU) period for approximately 2 years. Participants will continue in long-term follow-up (LTFU) for 15 years from LYL314 treatment.

Conditions

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Relapsed Non-Hodgkin Lymphoma Refractory Non-Hodgkin Lymphoma Non-Hodgkin Lymphoma Large B-cell Lymphoma

Keywords

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PMBCL High-grade B-cell lymphoma HGBL follicular lymphoma Grade 3B large cell follicular lymphoma Aggressive B-cell NHL Refractory Aggressive B-Cell Lymphoma Refractory B-Cell Non-Hodgkin Lymphoma Lymphoma, Non-Hodgkin Lymphoma CAR T-cell Non-Hodgkin Lymphoma CD19/20 CD19 CD20 NHL Diffuse Large B-cell lymphoma DLBCL Transformed follicular lymphoma TFL Primary mediastinal B-cell lymphoma Lymphoma, Large B-Cell, Diffuse Cyclophosphamide Fludarabine Lymphoma, Follicular Lymphoma, B-cell Immunosuppressive Agents Immunologic Factors Disease Attributes Immune System Diseases Recurrence PiNACLE

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

SEQUENTIAL

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Phase 1 CAR T naïve (Cohort 1)

Group Type EXPERIMENTAL

LYL314

Intervention Type DRUG

CAR T-cell therapy

Fludarabine

Intervention Type DRUG

Conditioning chemotherapy

Cyclophosphamide

Intervention Type DRUG

Conditioning chemotherapy

Phase 1 CAR T experienced (Cohort 2)

Group Type EXPERIMENTAL

LYL314

Intervention Type DRUG

CAR T-cell therapy

Fludarabine

Intervention Type DRUG

Conditioning chemotherapy

Cyclophosphamide

Intervention Type DRUG

Conditioning chemotherapy

Phase 1 Refractory disease or relapse within one year of first-line therapy (Cohort 3)

Group Type EXPERIMENTAL

LYL314

Intervention Type DRUG

CAR T-cell therapy

Fludarabine

Intervention Type DRUG

Conditioning chemotherapy

Cyclophosphamide

Intervention Type DRUG

Conditioning chemotherapy

Phase 1 Received T-cell engager (TCE) therapy (Cohort 4)

Group Type EXPERIMENTAL

LYL314

Intervention Type DRUG

CAR T-cell therapy

Fludarabine

Intervention Type DRUG

Conditioning chemotherapy

Cyclophosphamide

Intervention Type DRUG

Conditioning chemotherapy

Phase 1 Receiving first-line treatment for high-risk large B-cell lymphoma (Cohort 5)

Group Type EXPERIMENTAL

LYL314

Intervention Type DRUG

CAR T-cell therapy

Fludarabine

Intervention Type DRUG

Conditioning chemotherapy

Cyclophosphamide

Intervention Type DRUG

Conditioning chemotherapy

Phase 2 CAR T naïve (Cohort 1)

Single dose determined during Phase 1.

Group Type EXPERIMENTAL

LYL314

Intervention Type DRUG

CAR T-cell therapy

Fludarabine

Intervention Type DRUG

Conditioning chemotherapy

Cyclophosphamide

Intervention Type DRUG

Conditioning chemotherapy

Interventions

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LYL314

CAR T-cell therapy

Intervention Type DRUG

Fludarabine

Conditioning chemotherapy

Intervention Type DRUG

Cyclophosphamide

Conditioning chemotherapy

Intervention Type DRUG

Eligibility Criteria

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Inclusion Criteria

1. Age 18 years or older at time of informed consent
2. Willing and able to provide written informed consent
3. Histologically confirmed aggressive NHL, including the following types defined by the World Health Organization (WHO 2017):

* DLBCL
* DLBCL arising from follicular lymphoma (transformed FL, tFL)
* Primary mediastinal (thymic) large B-cell lymphoma (PMBCL)
* High-grade large B-cell lymphoma with or without MYC and BCL2 and/or BCL6 rearrangement (HGBL)
* Grade 3B follicular lymphoma/Large cell follicular lymphoma (FL3B)
4. Received at least two prior lines of therapy for Cohorts 1, 2, and 4 and one prior line of therapy for Cohort 3. Prior therapy must have included:

* Anti-CD20 monoclonal antibody, and
* An anthracycline containing chemotherapy regimen
* Participants with tFL must have received at least one of their prior lines of therapy after transformation to DLBCL

4b. Cohort 5 (High-risk first-line) participants must have high-risk large B-cell lymphoma
5. Relapsed or refractory disease, defined by the following:

* Disease progression after last regimen (including salvage therapy after autologous stem cell transplantation \[ASCT\]). In participants who have only received front-line therapy, progression should be ≤ 12 months of first-line therapy (applicable for Cohort 3)
* In patients who received one line of therapy, refractory disease is defined as failure to achieve at least a PR after at least 4 cycles of therapy (applicable for Cohort 3)
* In patients who received two or more lines of therapy (Cohorts 1, 2, and 4), refractory disease is defined as failure to achieve a CR to last line of therapy (including CAR T and/or salvage therapy).
6. At least 1 measurable lesion (per Lugano classification). Lesions that have been previously irradiated will be considered measurable only if progression has been documented following completion of radiation therapy
7. Eastern Cooperative Oncology Group (ECOG) performance status of 0 or 1 or ECOG 0 to 2 (Cohort 5)
8. Absolute neutrophil count (ANC) ≥ 1000/uL
9. Platelet count ≥ 50,000/uL
10. Absolute lymphocyte count (ALC) ≥ 200/uL

Other protocol-defined criteria apply.

Exclusion Criteria

1. History of malignancy other than non-melanoma skin cancer or carcinoma in situ (e.g., cervix, bladder, breast) unless disease-free for at least 3 years. Participants who have received therapy for a prior malignancy within the prior 3 years, e.g., in the adjuvant setting, are not excluded
2. Active central nervous system (CNS) involvement by malignancy on magnetic resonance imaging (MRI) or by lumbar puncture. Participants with prior evidence of brain metastasis treated at least 8 weeks prior to enrollment will not be excluded for participation if CNS disease is deemed stable at the time of study enrollment
3. History of cardiac lymphoma involvement or Epstein-Barr virus (EBV)+ lymphoma
4. Ongoing or impending oncologic emergency (e.g., tumor mass effect, tumor lysis syndrome, known vascular invasion)
5. Received the following therapies in the specified time frame prior to enrollment/leukapheresis

1. Any systemic therapy within 2 weeks
2. Any systemic inhibitory/stimulatory immune checkpoint molecule therapy within 3 half-lives prior to enrollment (e.g., ipilimumab, nivolumab, pembrolizumab, atezolizumab, OX40 agonists, 4-1BB agonists)
3. Fludarabine within 12 weeks
4. Alemtuzumab, bendamustine or antithymocyte globuline (ATG) within 6 months
5. Any T cell engager/bispecific antibody therapy such as CD20/CD3 or CD19/CD3 bispecific antibodies within 4 weeks
6. Any experimental therapy within 4 weeks or 5 half-lives (whichever is shorter)
6. Received radiation therapy within 3 weeks prior to enrollment/leukapheresis
7. Experiencing non-hematologic toxicities due to prior therapy. Exceptions include: stable and recovered to grade ≤ 1 or non-clinically significant toxicities such as (1) alopecia, (2) toxicities where Grade 2 is solely defined by participant receiving hormone replacement therapy for endocrinopathies resulting from previous checkpoint inhibitor therapy, (3) Grade 2 lymphopenia, and (4) hearing loss or Grade 2 neuropathy associated with prior treatment with taxanes or platinating agents
8. History of allogeneic stem cell or solid organ transplantation
9. Receipt of autologous stem cell transplantation within 6 weeks prior to enrollment/leukapheresis
10. History of prior genetically modified cell therapy other than a product targeting CD19 with an FMC63-based CAR (e.g., axicabtagene ciloleucel (axi-cel), tisagenlecleucel (tisa-cel), or lisocabtagene maraleucel (liso-cel). For all other CAR T cell therapy treatments, discussion with the Sponsor's Medical Monitor is required
11. Primary immunodeficiency
12. History of autoimmune disease (e.g., Crohn's disease, rheumatoid arthritis, systemic lupus) resulting in end organ injury or requiring systemic immunosuppression/systemic disease modifying agents within the last 2 years. Participants who have other autoimmune condition(s) considered to be associated with underlying malignancy may be enrolled in the study after discussion with and approval of the Medical Monitor.

Other protocol-defined criteria apply.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Lyell Immunopharma, Inc.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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University of California-Irvine Medical Center

Irvine, California, United States

Site Status RECRUITING

Cedars-Sinai Medical Center

Los Angeles, California, United States

Site Status RECRUITING

University of California, Los Angeles (UCLA) Medical Center

Los Angeles, California, United States

Site Status RECRUITING

Scripps Clinic

San Diego, California, United States

Site Status RECRUITING

Colorado Blood Cancer Institute

Denver, Colorado, United States

Site Status RECRUITING

Augusta University Medical Center

Augusta, Georgia, United States

Site Status RECRUITING

Indiana Blood and Marrow Transplantation

Indianapolis, Indiana, United States

Site Status RECRUITING

University of Iowa

Iowa City, Iowa, United States

Site Status RECRUITING

University of Louisville Brown Cancer Center

Louisville, Kentucky, United States

Site Status RECRUITING

Louisiana State University Health Sciences Center

Shreveport, Louisiana, United States

Site Status RECRUITING

Corewell Health

Grand Rapids, Michigan, United States

Site Status RECRUITING

University of Nebraska Medical Center

Omaha, Nebraska, United States

Site Status RECRUITING

University of New Mexico Comprehensive Cancer Center

Albuquerque, New Mexico, United States

Site Status RECRUITING

Montefiore Medical Center

The Bronx, New York, United States

Site Status RECRUITING

University of Cincinnati (UC) Physicians Company, LLC

Cincinnati, Ohio, United States

Site Status RECRUITING

Lehigh Valley Topper Cancer Center Institute

Allentown, Pennsylvania, United States

Site Status RECRUITING

Thomas Jefferson University

Philadelphia, Pennsylvania, United States

Site Status RECRUITING

Baylor University Medical Center

Dallas, Texas, United States

Site Status RECRUITING

Texas Transplant Institute

San Antonio, Texas, United States

Site Status RECRUITING

Huntsman Cancer Institute

Salt Lake City, Utah, United States

Site Status RECRUITING

Intermountain Healthcare

Salt Lake City, Utah, United States

Site Status RECRUITING

Virginia Oncology Associates

Norfolk, Virginia, United States

Site Status RECRUITING

Virginia Commonwealth University-Massey Cancer Center

Richmond, Virginia, United States

Site Status RECRUITING

Medical College of Wisconsin

Milwaukee, Wisconsin, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Lyell Immunopharma Inc.

Role: CONTACT

Phone: 888-707-7917

Email: [email protected]

Other Identifiers

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LYL314-101

Identifier Type: -

Identifier Source: org_study_id