Trial Outcomes & Findings for Aging and Gastrointestinal Barrier Function (NCT NCT05816551)

NCT ID: NCT05816551

Last Updated: 2025-01-22

Results Overview

In vivo gastrointestinal permeability measured by quantities of orally ingested multi-sugar drink excreted in urine. The urinary recovery of each ingested sugar (lactulose, sucrose and rhamnose) was determined by multiplying the measured concentration of each sugar by the total volume of urine collected and dividing by the dose administered. Since lactulose is degraded in the colon, we used the ratio urine lactulose to rhamnose (L/R) to determine small intestinal barrier permeability.

Recruitment status

COMPLETED

Study phase

NA

Target enrollment

22 participants

Primary outcome timeframe

"Visit 1": 3-5 hours after ingestion of multiple sugar drink.

Results posted on

2025-01-22

Participant Flow

Participant milestones

Participant milestones
Measure
Younger Participants
Individuals aged 18-39 years Hyperthermia Trial Individuals will be exposed to a controlled hyperthermia trial during "Visit 2" of the protocol. Control Trial Individuals will be exposed to a control trial (no hyperthermia) during "Visit 1" of the protocol.
Older Participants
Individuals aged over 65 years Hyperthermia Trial Individuals will be exposed to a controlled hyperthermia trial during "Visit 2" of the protocol. Control Trial Individuals will be exposed to a control trial (no hyperthermia) during "Visit 1" of the protocol.
Overall Study
STARTED
12
10
Overall Study
Control Trial
11
9
Overall Study
Hyperthermia Trial
12
9
Overall Study
COMPLETED
9
9
Overall Study
NOT COMPLETED
3
1

Reasons for withdrawal

Reasons for withdrawal
Measure
Younger Participants
Individuals aged 18-39 years Hyperthermia Trial Individuals will be exposed to a controlled hyperthermia trial during "Visit 2" of the protocol. Control Trial Individuals will be exposed to a control trial (no hyperthermia) during "Visit 1" of the protocol.
Older Participants
Individuals aged over 65 years Hyperthermia Trial Individuals will be exposed to a controlled hyperthermia trial during "Visit 2" of the protocol. Control Trial Individuals will be exposed to a control trial (no hyperthermia) during "Visit 1" of the protocol.
Overall Study
Withdrawal by Subject
0
1
Overall Study
Withdrawn by Primary Investigator
3
0

Baseline Characteristics

Aging and Gastrointestinal Barrier Function

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Younger Participants
n=9 Participants
Individuals aged 18-39 years Hyperthermia Trial: Individuals will be exposed to a controlled hyperthermia trial. Control Trial: Individuals will be exposed to a control trial (no hyperthermia).
Older Participants
n=9 Participants
Individuals aged over 65 years Hyperthermia Trial: Individuals will be exposed to a controlled hyperthermia trial. Control Trial: Individuals will be exposed to a control trial (no hyperthermia).
Total
n=18 Participants
Total of all reporting groups
Age, Categorical
<=18 years
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Age, Categorical
Between 18 and 65 years
9 Participants
n=5 Participants
0 Participants
n=7 Participants
9 Participants
n=5 Participants
Age, Categorical
>=65 years
0 Participants
n=5 Participants
9 Participants
n=7 Participants
9 Participants
n=5 Participants
Age, Continuous
32 years
STANDARD_DEVIATION 3 • n=5 Participants
72 years
STANDARD_DEVIATION 3 • n=7 Participants
52 years
STANDARD_DEVIATION 21 • n=5 Participants
Sex: Female, Male
Female
3 Participants
n=5 Participants
3 Participants
n=7 Participants
6 Participants
n=5 Participants
Sex: Female, Male
Male
6 Participants
n=5 Participants
6 Participants
n=7 Participants
12 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
9 Participants
n=5 Participants
9 Participants
n=7 Participants
18 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Asian
2 Participants
n=5 Participants
0 Participants
n=7 Participants
2 Participants
n=5 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Black or African American
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
White
7 Participants
n=5 Participants
9 Participants
n=7 Participants
16 Participants
n=5 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Region of Enrollment
United States
9 participants
n=5 Participants
9 participants
n=7 Participants
18 participants
n=5 Participants

PRIMARY outcome

Timeframe: "Visit 1": 3-5 hours after ingestion of multiple sugar drink.

In vivo gastrointestinal permeability measured by quantities of orally ingested multi-sugar drink excreted in urine. The urinary recovery of each ingested sugar (lactulose, sucrose and rhamnose) was determined by multiplying the measured concentration of each sugar by the total volume of urine collected and dividing by the dose administered. Since lactulose is degraded in the colon, we used the ratio urine lactulose to rhamnose (L/R) to determine small intestinal barrier permeability.

Outcome measures

Outcome measures
Measure
Younger Participants
n=9 Participants
Individuals aged 18-39 years Hyperthermia Trial: Individuals will be exposed to a controlled hyperthermia trial. Control Trial: Individuals will be exposed to a control trial (no hyperthermia).
Older Participants
n=9 Participants
Individuals aged over 65 years Hyperthermia Trial: Individuals will be exposed to a controlled hyperthermia trial. Control Trial: Individuals will be exposed to a control trial (no hyperthermia).
Log Lactulose/Rhamnose Following Control Trial
0.97 Ratio
Standard Deviation 0.39
0.79 Ratio
Standard Deviation 0.30

PRIMARY outcome

Timeframe: "Visit 2": 3-5 hours after ingestion of multiple sugar drink.

In vivo gastrointestinal permeability measured by quantities of orally ingested multi-sugar drink excreted in urine. The urinary recovery of each ingested sugar (lactulose, sucrose and rhamnose) was determined by multiplying the measured concentration of each sugar by the total volume of urine collected and dividing by the dose administered. Since lactulose is degraded in the colon, we used the ratio urine lactulose to rhamnose (L/R) to determine small intestinal barrier permeability.

Outcome measures

Outcome measures
Measure
Younger Participants
n=9 Participants
Individuals aged 18-39 years Hyperthermia Trial: Individuals will be exposed to a controlled hyperthermia trial. Control Trial: Individuals will be exposed to a control trial (no hyperthermia).
Older Participants
n=9 Participants
Individuals aged over 65 years Hyperthermia Trial: Individuals will be exposed to a controlled hyperthermia trial. Control Trial: Individuals will be exposed to a control trial (no hyperthermia).
Log Lactulose/Rhamnose Following Hyperthermia Trial
1.22 Ratio
Standard Deviation 0.32
1.21 Ratio
Standard Deviation 0.29

PRIMARY outcome

Timeframe: "Visit 1": 3-5 hours after ingestion of multiple sugar drink.

The urinary recovery of sucrose was determined by multiplying the measured concentration of sucrose by the total volume of urine collected and dividing by the dose administered. Sucrose is broken down rapidly in the duodenum, thus we used the urinary excretion of sucrose to assess and gastroduodenal permeability.

Outcome measures

Outcome measures
Measure
Younger Participants
n=9 Participants
Individuals aged 18-39 years Hyperthermia Trial: Individuals will be exposed to a controlled hyperthermia trial. Control Trial: Individuals will be exposed to a control trial (no hyperthermia).
Older Participants
n=9 Participants
Individuals aged over 65 years Hyperthermia Trial: Individuals will be exposed to a controlled hyperthermia trial. Control Trial: Individuals will be exposed to a control trial (no hyperthermia).
Log Sucrose Following Control Trial
2.68 log(ng/mL)
Standard Deviation 1.11
1.74 log(ng/mL)
Standard Deviation 1.66

PRIMARY outcome

Timeframe: "Visit 2": 3-5 hours after ingestion of multiple sugar drink.

The urinary recovery of sucrose was determined by multiplying the measured concentration of sucrose by the total volume of urine collected and dividing by the dose administered. Sucrose is broken down rapidly in the duodenum, thus we used the urinary excretion of sucrose to assess and gastroduodenal permeability.

Outcome measures

Outcome measures
Measure
Younger Participants
n=9 Participants
Individuals aged 18-39 years Hyperthermia Trial: Individuals will be exposed to a controlled hyperthermia trial. Control Trial: Individuals will be exposed to a control trial (no hyperthermia).
Older Participants
n=9 Participants
Individuals aged over 65 years Hyperthermia Trial: Individuals will be exposed to a controlled hyperthermia trial. Control Trial: Individuals will be exposed to a control trial (no hyperthermia).
Log Sucrose Following Hyperthermia Trial
2.83 log(ng/mL)
Standard Deviation 1.10
3.44 log(ng/mL)
Standard Deviation 0.50

SECONDARY outcome

Timeframe: "Visit 2": At 0 minutes and 50-90minutes into the hyperthermia trial.

Core temperature was measured using an orally ingestible telemetric pill that was taken no less than 1 hour before the baseline period.

Outcome measures

Outcome measures
Measure
Younger Participants
n=9 Participants
Individuals aged 18-39 years Hyperthermia Trial: Individuals will be exposed to a controlled hyperthermia trial. Control Trial: Individuals will be exposed to a control trial (no hyperthermia).
Older Participants
n=9 Participants
Individuals aged over 65 years Hyperthermia Trial: Individuals will be exposed to a controlled hyperthermia trial. Control Trial: Individuals will be exposed to a control trial (no hyperthermia).
Change in Core Temperature During Hyperthermia Trial
2.01 °C
Standard Deviation .14
1.95 °C
Standard Deviation .23

Adverse Events

Younger Participants Hyperthermia Trial

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Older Participants Hyperthermia Trial

Serious events: 0 serious events
Other events: 1 other events
Deaths: 0 deaths

Younger Participants Control Trial

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Older Participants Control Trial

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
Younger Participants Hyperthermia Trial
n=12 participants at risk
Individuals aged 18-39 years Hyperthermia Trial: Individuals will be exposed to a controlled hyperthermia trial.
Older Participants Hyperthermia Trial
n=9 participants at risk
Individuals aged over 65 years Hyperthermia Trial: Individuals will be exposed to a controlled hyperthermia trial.
Younger Participants Control Trial
n=11 participants at risk
Individuals aged 18-39 years Control Trial: Individuals will be exposed to a control trial (no hyperthermia).
Older Participants Control Trial
n=9 participants at risk
Individuals aged over 65 years Control Trial: Individuals will be exposed to a control trial (no hyperthermia).
General disorders
Syncope
0.00%
0/12 • During the two trials of the experiment or approximately 2 weeks.
11.1%
1/9 • Number of events 1 • During the two trials of the experiment or approximately 2 weeks.
0.00%
0/11 • During the two trials of the experiment or approximately 2 weeks.
0.00%
0/9 • During the two trials of the experiment or approximately 2 weeks.

Additional Information

Craig Crandall, PhD, Primary Investigator

Institute for Exercise and Environmental Medicine

Phone: 214-345-4623

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place