The Influence of Probiotic Supplementation on the Severity of Anxiety and Depressive Symptoms, as Well as the Function and Composition of Gut Microbiota, Metabolic, Inflammation, and Oxidative Stress Markers in Patients With Depression

NCT ID: NCT05717946

Last Updated: 2023-02-08

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

PHASE2

Total Enrollment

100 participants

Study Classification

INTERVENTIONAL

Study Start Date

2021-01-04

Study Completion Date

2023-12-31

Brief Summary

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The aim of this study is to gather empirical evidence which will enable to evaluate the impact of probiotic supplementation on the severity of anxiety and depressive symptoms, function, and composition of gut microbiota, metabolic parameters, inflammation, and oxidative stress markers in patients with diagnosed depressive disorders.

The designed study will be prospective, randomized, placebo-controlled, and double-blind. The intervention period will last 8 weeks for each patient. The study will be conducted on 100 patients in total, who will be randomly divided into two groups, consisting of 50 patients each.

Patients included in Group I (PRO-D) will receive one capsule daily containing a probiotic mixture at a daily dose of 3×109 colony-forming units (CFU). The probiotic will be composed of two bacteria strains: Lactobacillus helveticus Rosell®-52, Bifidobacterium longum Rosell®-175, and excipients: potato starch, magnesium stearate, and the capsule shell, made of hydroxypropylmethylcellulose.

Patients included in Group II (PLC-D) will receive daily the same capsule, containing only excipients: potato starch, maltodextrin, and the capsule shell. The color, smell, and taste of the placebo will not be different from those included in the probiotic capsule.

Patients will be considered compliant if they consume \>= 80% of the supplements.

The primary outcome measures will be the severity of depressive anxiety and stress symptoms assessed with Montgomery-Åsberg Depression Rating Scale (MADRS) and the Depression, Anxiety, Stress Scale (DASS) with subscales scores, the quality of life level assessed with the WHOQOL-BREF instrument. The secondary outcomes measures will include: blood pressure (BP), body mass index (BMI) and waist circumference (WC) measures, fasting glucose (fGlc), HDL cholesterol (HDL-C), triglycerides (TG), white blood cells count (WBC), neutrofiles, serum levels of C-reactive protein (CRP), the level of faecal SCFAs, faecal microbiota α-diversity and the level of oxidative stress parameters (total antioxidant capacity (TAC) and malondialdehyde (MDA)) in the blood serum.

Detailed Description

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Conditions

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Depression Anxiety

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Prospective, randomized, placebo-controlled, and double-blind trial
Primary Study Purpose

TREATMENT

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors
double-blind trial, independent researcher is responsible for randomization and blinding.

Study Groups

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Group I "PRO-D"

50 patients with diagnosed depressive disorders, meeting the criteria of International Classification of Diseases (ICD-11) for 6A70-73, 6A7Y, 6A7Z, taking a probiotic composed of two bacteria strains: Lactobacillus helveticus Rosell and Bifidobacterium longum Rosell.

Group Type EXPERIMENTAL

Probiotic composed of two bacteria strains: Lactobacillus helveticus Rosell and Bifidobacterium longum Rosell.

Intervention Type DIETARY_SUPPLEMENT

We plan to investigate the impact of the Lactobacillus helveticus Rosell and Bifidobacterium longum Rosell on clinical and biochemical parameters in the experimental group.

Group II "PLC-D"

50 patients with diagnosed depressive disorders, meeting the criteria of International Classification of Diseases (ICD-11) for 6A70-73, 6A7Y, 6A7Z, taking a placebo.

Group Type PLACEBO_COMPARATOR

control group

Intervention Type OTHER

We plan to investigate the impact of a placebo on clinical and biochemical parameters in the control group.

Interventions

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Probiotic composed of two bacteria strains: Lactobacillus helveticus Rosell and Bifidobacterium longum Rosell.

We plan to investigate the impact of the Lactobacillus helveticus Rosell and Bifidobacterium longum Rosell on clinical and biochemical parameters in the experimental group.

Intervention Type DIETARY_SUPPLEMENT

control group

We plan to investigate the impact of a placebo on clinical and biochemical parameters in the control group.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

1. Depressive disorders diagnosed according to ICD-11,
2. Age between 18 - 70 years,
3. MADRS score \>=13,
4. Antidepressant and antianxiety medications not changed 3 weeks prior to the recruitment visit.

Exclusion Criteria

1. Pregnancy,
2. An infection/vaccination and/or treatment with antibiotics in the previous 4 weeks,
3. Supplementation with pro- or prebiotics in the previous 4 weeks,
4. Having a diagnosis of autoimmune, serious immunocompromised, inflammatory bowel diseases, cancer, IgE-dependent allergy, or severe kidney failure in the previous 4 weeks,
5. body mass index (BMI)\> 35,
6. glomerular filtration rate (GFR)\< 30 ml/min/1,72 m2,
7. unstable thyroid dysfunction (TSH \< 0,27 or \> 4,2 μIU/ml) in the previous 4 weeks,
8. Psychiatric comorbidities (except specific personality disorder, additional specific anxiety disorder, and caffeine, and nicotine addiction),
9. Regular treatment (more than 3 days a week) with PPIs, metformin, laxatives, systemic steroids, or NSAIDs in the previous 4 weeks,
10. Significant change in dietary pattern in the previous 4 weeks,
11. Significant change in daily physical activity or extreme sports activity in the previous 4 weeks,
12. Significant change in dietary supplementation in the previous 4 weeks,
13. Significant change in smoking pattern in the previous 4 weeks,
14. High risk of suicide,
15. Is participating in, or has recently participated in, another research study involving an intervention that may alter outcomes of interest to this study,
16. Any other condition or situation which, in the view of investigators, would affect the compliance or safety of the individual taking part.

Reasons for the participant to be discontinued from the study:

1. Withdrawal of informed consent,
2. An infection/vaccination and/or treatment with antibiotics during the trial,
3. Consuming any other than studied probiotics during the trial,
4. Lack of compliance with the probiotic supplementation,
5. Any change in the drug regimen during the study,
7. Any serious adverse event during the trial.
Minimum Eligible Age

18 Years

Maximum Eligible Age

70 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Medical University of Lodz

OTHER

Sponsor Role lead

Responsible Party

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Dominik Strzelecki

Professor, MD, PhD

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Dominik Strzelecki, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

Medical University of Lodz

Locations

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Central Teaching Hospital, Medical University of Lodz

Lodz, , Poland

Site Status RECRUITING

Countries

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Poland

Central Contacts

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Dominik Strzelecki, MD, PhD

Role: CONTACT

+48426757371

Anna Skowrońska, MD

Role: CONTACT

+48426757371

Facility Contacts

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Dominik Strzelecki, MD, PhD

Role: primary

+48426757371

Anna Skowrońska, MD

Role: backup

+48426757371

Other Identifiers

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MULodzAff_1

Identifier Type: -

Identifier Source: org_study_id

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