Porphyromonas Gingivalis and Severity of Fibrosis in Patients With Non-alcoholic Fatty Liver Disease

NCT ID: NCT05654129

Last Updated: 2022-12-16

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

182 participants

Study Classification

INTERVENTIONAL

Study Start Date

2022-12-05

Study Completion Date

2023-12-05

Brief Summary

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Many studies suggest that Porphyromonas gingivalis, a virulent periodontopathogenic bacterium, is associated with many systemic diseases such as cardiovascular diseases. In addition, a recent study showed the impact of Porphyromonas gingivalis on Non-Alcoholic Steatosis Liver Disease (NASH). It would be responsible for aggravation of non-alcoholic fatty liver disease (NAFLD) by stimulating inflammation in the damaged liver tissue.

Detailed Description

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Non-alcoholic fatty liver disease is becoming the leading cause of chronic liver disease with a prevalence of 20% worldwide. The prognosis depends on the degree of fibrosis: patients with a low degree of fibrosis (F0-F2) have a good prognosis unlike those with severe fibrosis or cirrhosis (F3-F4), exposed to excess mortality from cardiovascular diseases , cancers, and complications of cirrhosis. The diagnosis of the fibrosis stage is histological but the worsening of the fibrosis remains unknown.

The intestinal microbiota is an etiological factor in NAFLD and dysbiosis is associated with the severity of fibrosis. There is also a physiopathological rationale between oral bacterial microbiota and NAFLD. The oral microbiota is very rich, it corresponds to a reservoir of 1010 bacteria of Gram-negative bacteria (BGN). Its dysbiosis causes oral infections like periodontal diseases, immuno-infectious pathologies linked to an imbalance between the bacterial etiological factor and the host's immune defenses.

Many studies suggest that Porphyromonas gingivalis, a virulent periodontopathogenic bacterium, is associated with many systemic diseases such as cardiovascular diseases. In addition, a recent study showed the impact of Porphyromonas gingivalis on Non-Alcoholic Steatosis Liver Disease (NASH). It would be responsible for aggravation of non-alcoholic fatty liver disease (NAFLD) by stimulating inflammation in the damaged liver tissue.

the hypothesis that the salivary levels of Porphyromonas Gingivalis could be associated with the degree of severity of fibrosis in NAFLD patients, and would constitute a new therapeutic target for the evaluation of fibrosis. This work would open new perspectives in treatment strategies.

Conditions

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Non-Alcoholic Fatty Liver Disease

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

people with stage F1-F2 NAFLD versus people with stage F3-F4
Primary Study Purpose

OTHER

Blinding Strategy

NONE

Study Groups

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F1-F2 NAFLD

Patients with NAFLD stage 1 or 2 confirmed by a biopsy less than 1 year old

Group Type OTHER

saliva samples

Intervention Type OTHER

saliva samples (non-invasive, in the sites evaluated during the partial survey) will be perform by one of the investigators .

medical questionnaire

Intervention Type OTHER

a complete medical questionnaire will be carried out (general, lifestyle and quality of life) will be perform by one of the investigators .

F3-F4 NAFLD

Patients with NAFLD stage 3 or 4 confirmed by a biopsy less than 1 year old

Group Type OTHER

saliva samples

Intervention Type OTHER

saliva samples (non-invasive, in the sites evaluated during the partial survey) will be perform by one of the investigators .

medical questionnaire

Intervention Type OTHER

a complete medical questionnaire will be carried out (general, lifestyle and quality of life) will be perform by one of the investigators .

Interventions

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saliva samples

saliva samples (non-invasive, in the sites evaluated during the partial survey) will be perform by one of the investigators .

Intervention Type OTHER

medical questionnaire

a complete medical questionnaire will be carried out (general, lifestyle and quality of life) will be perform by one of the investigators .

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* subjects with NAFLD confirmed by a biopsy less than 1 year old
* subjects over the age of 18
* subjects able to receive information on the course of the study and to understand the information form to participate in the study
* the subjects who gave their non-objection to participate in the study

Exclusion Criteria

* Having another associated cause of liver disease or steatosis such as viral infections and autoimmune disease
* With alcohol consumption \>30g/d (men) or 20g/d (women)
* With a history of cirrhosis decompensation
* Having taken antibiotics, prebiotics or probiotics in the month prior to inclusion
* With chronic or acute gastrointestinal disease
* With a history of gastrointestinal surgery modifying the anatomy
* During pregnancy or breastfeeding
* With a history of oral surgery in the month prior to inclusion
* For whom oral surgery is planned between the collection of the non-objection and the inclusion in the odontology service
* At risk of infection (existence of one or more known chronic infectious pathologies) and/or chronic renal failure (creatinine clearance \< 60ml/min).
* Suffering from a general pathology contraindicating the performance of diagnostic procedures such as periodontal probing (patients at high risk of infective endocarditis according to the French National Agency for the Safety of Medicines and Health Products ANSM)
* Under legal protection, dependent, under guardianship or curatorship
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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University Hospital, Toulouse

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Vincent BLASCO-BAQUE

Role: PRINCIPAL_INVESTIGATOR

University Hospital, Toulouse

Locations

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CHU Toulouse

Toulouse, , France

Site Status RECRUITING

Countries

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France

Central Contacts

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Vincent BLASCO-BAQUE

Role: CONTACT

Phone: 0675188124

Email: [email protected]

Cathy Nabet

Role: CONTACT

Email: [email protected]

Facility Contacts

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Vincent BLASCO-BAQUE

Role: primary

Other Identifiers

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RC31/19/0043

Identifier Type: -

Identifier Source: org_study_id