The Impact of the Distribution of Adipose Tissue on the Occurrence of Metabolic Disorders and the Level of Cardiopulmonary Fitness

NCT ID: NCT05612282

Last Updated: 2022-11-10

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

91 participants

Study Classification

OBSERVATIONAL

Study Start Date

2016-11-11

Study Completion Date

2017-10-31

Brief Summary

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The aim of the research was to assess the impact of the distribution of abdominal fat (subcutaneous SAT and visceral VAT estimated at the height of the navel) on selected metabolic parameters and on specific parameters of cardiopulmonary fitness in terms of people with obesity.

Detailed Description

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The research was carried out in 3 stages. Stage 1 was carried out at the Department of Dietetics and Clinical Nutrition of the UMB. During the visit, the subjects were provided with detailed information concerning the research and every participant signed a voluntary consent to participate in the research. Furthermore, an interview questionnaire concerning the duration of obesity and past diseases was collected, and an assessment of the status of nutrition based on anthropometric measurements was carried out, and a body composition analysis was performed using the bioelectroimpedance method using the Maltron BioScan 920-2 analyzer. Then, patients were issued a referral for laboratory examinations. Stage 2 was carried out at the Medical Laboratory at the University Clinical Hospital in Bialystok, where blood was taken from the ulnar vein (in the amount of 15ml). The concentrations of the following parameters were determined from the blood serum: fasting glucose, fasting insulin, total cholesterol, LDL cholesterol fraction, HDL cholesterol fraction, triglycerides, C-reactive protein (CRP), uric acid, creatinine, and aminotrasferases: alanine (ALT) and aspartate (AST). On the basis of fasting glucose and fasting insulin, the HOMA - IR insulin resistance index was calculated. Stage 3 was carried out at the Maniac Gym Fitness Club in Bialystok, where cardiopulmonary fitness was assessed using the Modified Bruce protocol.

Conditions

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Obesity, Abdominal

Study Design

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Observational Model Type

CASE_CROSSOVER

Study Time Perspective

PROSPECTIVE

Study Groups

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G1a - women

The G1a group consisted of women with obesity, in whom no additional components of the metabolic syndrome were found (n =16)

body composition analysis

Intervention Type DIAGNOSTIC_TEST

A body composition analysis was performed using the bioelectroimpedance method using the Maltron BioScan 920-2 analyzer

anthropometric measurements

Intervention Type OTHER

An assessment of the status of nutrition based on anthropometric measurements was carried out.

Interview questionnaire

Intervention Type OTHER

An interview questionnaire concerning the duration of obesity and past diseases was collected. Moreover, the diet and nutritional status were assessed.

biochemical parameters

Intervention Type DIAGNOSTIC_TEST

The concentrations of the following parameters were determined from the blood serum: fasting glucose, fasting insulin, total cholesterol, LDL cholesterol fraction, HDL cholesterol fraction, triglycerides, C-reactive protein (CRP), uric acid, creatinine, and aminotrasferases: alanine (ALT) and aspartate (AST). On the basis of fasting glucose and fasting insulin, the HOMA - IR insulin resistance index was calculated.

cardiopulmonary fitness test

Intervention Type DIAGNOSTIC_TEST

Cardiopulmonary fitness was assessed using the Modified Bruce protocol

nutritional interview

Intervention Type OTHER

A 7-day nutrition interview was collected. The supply of energy, protein (including amino acids), fat (including fatty acids), carbohydrates, dietary fiber, vitamins and minerals in the usual diet was assessed.

G1b - men

The G1b group consisted of men with obesity, in whom no additional components of the metabolic syndrome were founds (n =6)

body composition analysis

Intervention Type DIAGNOSTIC_TEST

A body composition analysis was performed using the bioelectroimpedance method using the Maltron BioScan 920-2 analyzer

anthropometric measurements

Intervention Type OTHER

An assessment of the status of nutrition based on anthropometric measurements was carried out.

Interview questionnaire

Intervention Type OTHER

An interview questionnaire concerning the duration of obesity and past diseases was collected. Moreover, the diet and nutritional status were assessed.

biochemical parameters

Intervention Type DIAGNOSTIC_TEST

The concentrations of the following parameters were determined from the blood serum: fasting glucose, fasting insulin, total cholesterol, LDL cholesterol fraction, HDL cholesterol fraction, triglycerides, C-reactive protein (CRP), uric acid, creatinine, and aminotrasferases: alanine (ALT) and aspartate (AST). On the basis of fasting glucose and fasting insulin, the HOMA - IR insulin resistance index was calculated.

cardiopulmonary fitness test

Intervention Type DIAGNOSTIC_TEST

Cardiopulmonary fitness was assessed using the Modified Bruce protocol

nutritional interview

Intervention Type OTHER

A 7-day nutrition interview was collected. The supply of energy, protein (including amino acids), fat (including fatty acids), carbohydrates, dietary fiber, vitamins and minerals in the usual diet was assessed.

G2a - women

The G2a group consisted of women with obesity, in whom only one additional component of the metabolic syndrome was found (e.g. triglycerides ≥ 150 mg/dl, HDL cholesterol in women \< 50 mg/dl, and in men \< 40 mg/dl, or glycaemia ≥ 100 mg/dl), but it was not a disease previously diagnosed and treated (n=19)

body composition analysis

Intervention Type DIAGNOSTIC_TEST

A body composition analysis was performed using the bioelectroimpedance method using the Maltron BioScan 920-2 analyzer

anthropometric measurements

Intervention Type OTHER

An assessment of the status of nutrition based on anthropometric measurements was carried out.

Interview questionnaire

Intervention Type OTHER

An interview questionnaire concerning the duration of obesity and past diseases was collected. Moreover, the diet and nutritional status were assessed.

biochemical parameters

Intervention Type DIAGNOSTIC_TEST

The concentrations of the following parameters were determined from the blood serum: fasting glucose, fasting insulin, total cholesterol, LDL cholesterol fraction, HDL cholesterol fraction, triglycerides, C-reactive protein (CRP), uric acid, creatinine, and aminotrasferases: alanine (ALT) and aspartate (AST). On the basis of fasting glucose and fasting insulin, the HOMA - IR insulin resistance index was calculated.

cardiopulmonary fitness test

Intervention Type DIAGNOSTIC_TEST

Cardiopulmonary fitness was assessed using the Modified Bruce protocol

nutritional interview

Intervention Type OTHER

A 7-day nutrition interview was collected. The supply of energy, protein (including amino acids), fat (including fatty acids), carbohydrates, dietary fiber, vitamins and minerals in the usual diet was assessed.

G2b - men

The G2a group consisted of men with obesity, in whom only one additional component of the metabolic syndrome was found (e.g. triglycerides ≥ 150 mg/dl, HDL cholesterol in women \< 50 mg/dl, and in men \< 40 mg/dl, or glycaemia ≥ 100 mg/dl), but it was not a disease previously diagnosed and treated (n=11)

body composition analysis

Intervention Type DIAGNOSTIC_TEST

A body composition analysis was performed using the bioelectroimpedance method using the Maltron BioScan 920-2 analyzer

anthropometric measurements

Intervention Type OTHER

An assessment of the status of nutrition based on anthropometric measurements was carried out.

Interview questionnaire

Intervention Type OTHER

An interview questionnaire concerning the duration of obesity and past diseases was collected. Moreover, the diet and nutritional status were assessed.

biochemical parameters

Intervention Type DIAGNOSTIC_TEST

The concentrations of the following parameters were determined from the blood serum: fasting glucose, fasting insulin, total cholesterol, LDL cholesterol fraction, HDL cholesterol fraction, triglycerides, C-reactive protein (CRP), uric acid, creatinine, and aminotrasferases: alanine (ALT) and aspartate (AST). On the basis of fasting glucose and fasting insulin, the HOMA - IR insulin resistance index was calculated.

cardiopulmonary fitness test

Intervention Type DIAGNOSTIC_TEST

Cardiopulmonary fitness was assessed using the Modified Bruce protocol

nutritional interview

Intervention Type OTHER

A 7-day nutrition interview was collected. The supply of energy, protein (including amino acids), fat (including fatty acids), carbohydrates, dietary fiber, vitamins and minerals in the usual diet was assessed.

G3a - women

Group G3a consisted of women who met the full criteria for diagnosing the metabolic syndrome (n=24)

body composition analysis

Intervention Type DIAGNOSTIC_TEST

A body composition analysis was performed using the bioelectroimpedance method using the Maltron BioScan 920-2 analyzer

anthropometric measurements

Intervention Type OTHER

An assessment of the status of nutrition based on anthropometric measurements was carried out.

Interview questionnaire

Intervention Type OTHER

An interview questionnaire concerning the duration of obesity and past diseases was collected. Moreover, the diet and nutritional status were assessed.

biochemical parameters

Intervention Type DIAGNOSTIC_TEST

The concentrations of the following parameters were determined from the blood serum: fasting glucose, fasting insulin, total cholesterol, LDL cholesterol fraction, HDL cholesterol fraction, triglycerides, C-reactive protein (CRP), uric acid, creatinine, and aminotrasferases: alanine (ALT) and aspartate (AST). On the basis of fasting glucose and fasting insulin, the HOMA - IR insulin resistance index was calculated.

cardiopulmonary fitness test

Intervention Type DIAGNOSTIC_TEST

Cardiopulmonary fitness was assessed using the Modified Bruce protocol

nutritional interview

Intervention Type OTHER

A 7-day nutrition interview was collected. The supply of energy, protein (including amino acids), fat (including fatty acids), carbohydrates, dietary fiber, vitamins and minerals in the usual diet was assessed.

G3b - men

Group G3a consisted of men who met the full criteria for diagnosing the metabolic syndrome (n=15)

body composition analysis

Intervention Type DIAGNOSTIC_TEST

A body composition analysis was performed using the bioelectroimpedance method using the Maltron BioScan 920-2 analyzer

anthropometric measurements

Intervention Type OTHER

An assessment of the status of nutrition based on anthropometric measurements was carried out.

Interview questionnaire

Intervention Type OTHER

An interview questionnaire concerning the duration of obesity and past diseases was collected. Moreover, the diet and nutritional status were assessed.

biochemical parameters

Intervention Type DIAGNOSTIC_TEST

The concentrations of the following parameters were determined from the blood serum: fasting glucose, fasting insulin, total cholesterol, LDL cholesterol fraction, HDL cholesterol fraction, triglycerides, C-reactive protein (CRP), uric acid, creatinine, and aminotrasferases: alanine (ALT) and aspartate (AST). On the basis of fasting glucose and fasting insulin, the HOMA - IR insulin resistance index was calculated.

cardiopulmonary fitness test

Intervention Type DIAGNOSTIC_TEST

Cardiopulmonary fitness was assessed using the Modified Bruce protocol

nutritional interview

Intervention Type OTHER

A 7-day nutrition interview was collected. The supply of energy, protein (including amino acids), fat (including fatty acids), carbohydrates, dietary fiber, vitamins and minerals in the usual diet was assessed.

Interventions

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body composition analysis

A body composition analysis was performed using the bioelectroimpedance method using the Maltron BioScan 920-2 analyzer

Intervention Type DIAGNOSTIC_TEST

anthropometric measurements

An assessment of the status of nutrition based on anthropometric measurements was carried out.

Intervention Type OTHER

Interview questionnaire

An interview questionnaire concerning the duration of obesity and past diseases was collected. Moreover, the diet and nutritional status were assessed.

Intervention Type OTHER

biochemical parameters

The concentrations of the following parameters were determined from the blood serum: fasting glucose, fasting insulin, total cholesterol, LDL cholesterol fraction, HDL cholesterol fraction, triglycerides, C-reactive protein (CRP), uric acid, creatinine, and aminotrasferases: alanine (ALT) and aspartate (AST). On the basis of fasting glucose and fasting insulin, the HOMA - IR insulin resistance index was calculated.

Intervention Type DIAGNOSTIC_TEST

cardiopulmonary fitness test

Cardiopulmonary fitness was assessed using the Modified Bruce protocol

Intervention Type DIAGNOSTIC_TEST

nutritional interview

A 7-day nutrition interview was collected. The supply of energy, protein (including amino acids), fat (including fatty acids), carbohydrates, dietary fiber, vitamins and minerals in the usual diet was assessed.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* primary obesity
* BMI ≥ 30kg/m2 - ≤ 39,99 kg/m2
* gender: women and men
* age: 20-65 lat

Exclusion Criteria

* secondry obesity
* BMI ≥ 40 kg/m2
* diabetes type 2 or insulin resistance (occurance or treatment)
* endocrine disorders
* eating disorders
* hormonal contraception/hormone replacement therapy
* after steroid therapy
* antiretroviral therapy
* musculoskeletal dysfunctions
* patients after surgery (min 3 months)
* bariatric surgery
* coronary artery disease
* cardiac pacemaker
* pregnancy
* breast - feeding
Minimum Eligible Age

20 Years

Maximum Eligible Age

65 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Medical University of Bialystok

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Katarzyna Witczak - Sawczuk, PhD

Role: STUDY_DIRECTOR

Medical University of Bialystok

Lucyna Ostrowska, Professor

Role: STUDY_CHAIR

Medical University of Bialystok

References

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Mongraw-Chaffin M, Foster MC, Anderson CAM, Burke GL, Haq N, Kalyani RR, Ouyang P, Sibley CT, Tracy R, Woodward M, Vaidya D. Metabolically Healthy Obesity, Transition to Metabolic Syndrome, and Cardiovascular Risk. J Am Coll Cardiol. 2018 May 1;71(17):1857-1865. doi: 10.1016/j.jacc.2018.02.055.

Reference Type BACKGROUND
PMID: 29699611 (View on PubMed)

Pimentel Ade C, Scorsatto M, de Oliveira GM, Rosa G, Luiz RR. Characterization of metabolically healthy obese Brazilians and cardiovascular risk prediction. Nutrition. 2015 Jun;31(6):827-33. doi: 10.1016/j.nut.2014.12.024. Epub 2015 Jan 10.

Reference Type BACKGROUND
PMID: 25933489 (View on PubMed)

Hoddy KK, Axelrod CL, Mey JT, Hari A, Beyl RA, Blair JB, Dantas WS, Kirwan JP. Insulin resistance persists despite a metabolically healthy obesity phenotype. Obesity (Silver Spring). 2022 Jan;30(1):39-44. doi: 10.1002/oby.23312. Epub 2021 Nov 24.

Reference Type BACKGROUND
PMID: 34816598 (View on PubMed)

Perreault M, Zulyniak MA, Badoud F, Stephenson S, Badawi A, Buchholz A, Mutch DM. A distinct fatty acid profile underlies the reduced inflammatory state of metabolically healthy obese individuals. PLoS One. 2014 Feb 10;9(2):e88539. doi: 10.1371/journal.pone.0088539. eCollection 2014.

Reference Type BACKGROUND
PMID: 24520395 (View on PubMed)

Kim H, Yoon E, Kim OY, Kim EM. Short-term Effects of Eating Behavior Modification on Metabolic Syndrome-Related Risks in Overweight and Obese Korean Adults. J Obes Metab Syndr. 2022 Mar 30;31(1):70-80. doi: 10.7570/jomes21074.

Reference Type BACKGROUND
PMID: 35296564 (View on PubMed)

Zhou YH, Guo Y, Wang F, Zhou CL, Tang CY, Tang HN, Yan DW, Zhou HD. Association of Sex Hormones and Fat Distribution in Men with Different Obese and Metabolic Statuses. Int J Gen Med. 2022 Feb 9;15:1225-1238. doi: 10.2147/IJGM.S351282. eCollection 2022.

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PMID: 35173463 (View on PubMed)

Numao S, So R, Matsuo T, Nakagaichi M, Tanaka K. A favorable metabolic profile in metabolically healthy obesity is associated with physical activity level rather than abdominal fat volume in Japanese males. J Phys Ther Sci. 2021 Feb;33(2):137-141. doi: 10.1589/jpts.33.137. Epub 2021 Feb 13.

Reference Type BACKGROUND
PMID: 33642688 (View on PubMed)

Matsha TE, Ismail S, Speelman A, Hon GM, Davids S, Erasmus RT, Kengne AP. Visceral and subcutaneous adipose tissue association with metabolic syndrome and its components in a South African population. Clin Nutr ESPEN. 2019 Aug;32:76-81. doi: 10.1016/j.clnesp.2019.04.010. Epub 2019 Jun 3.

Reference Type BACKGROUND
PMID: 31221294 (View on PubMed)

Osorio-Conles O, Vega-Beyhart A, Ibarzabal A, Balibrea JM, Vidal J, de Hollanda A. Biological Determinants of Metabolic Syndrome in Visceral and Subcutaneous Adipose Tissue from Severely Obese Women. Int J Mol Sci. 2022 Feb 21;23(4):2394. doi: 10.3390/ijms23042394.

Reference Type BACKGROUND
PMID: 35216509 (View on PubMed)

Schorr M, Dichtel LE, Gerweck AV, Valera RD, Torriani M, Miller KK, Bredella MA. Sex differences in body composition and association with cardiometabolic risk. Biol Sex Differ. 2018 Jun 27;9(1):28. doi: 10.1186/s13293-018-0189-3.

Reference Type BACKGROUND
PMID: 29950175 (View on PubMed)

Chashmniam S, Hashemi Madani N, Nobakht Mothlagh Ghoochani BF, Safari-Alighiarloo N, Khamseh ME. The metabolome profiling of obese and non-obese individuals: Metabolically healthy obese and unhealthy non-obese paradox. Iran J Basic Med Sci. 2020 Feb;23(2):186-194. doi: 10.22038/IJBMS.2019.37885.9004.

Reference Type BACKGROUND
PMID: 32405361 (View on PubMed)

Kaess BM, Pedley A, Massaro JM, Murabito J, Hoffmann U, Fox CS. The ratio of visceral to subcutaneous fat, a metric of body fat distribution, is a unique correlate of cardiometabolic risk. Diabetologia. 2012 Oct;55(10):2622-2630. doi: 10.1007/s00125-012-2639-5. Epub 2012 Aug 17.

Reference Type BACKGROUND
PMID: 22898763 (View on PubMed)

Other Identifiers

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N/ST/MN/17/003/3316

Identifier Type: -

Identifier Source: org_study_id

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