A Study to Evaluate Safety, Reactogenicity, and Immune Response of GVGH iNTS-TCV Vaccine Against Invasive Nontyphoidal Salmonella and Typhoid Fever

NCT ID: NCT05480800

Last Updated: 2025-11-28

Study Results

Results available

Outcome measurements, participant flow, baseline characteristics, and adverse events have been published for this study.

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Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1/PHASE2

Total Enrollment

155 participants

Study Classification

INTERVENTIONAL

Study Start Date

2022-09-13

Study Completion Date

2025-01-07

Brief Summary

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The purpose of this study is to assess the safety, reactogenicity, and immune response induced by the GlaxoSmithKline Biologicals SA (GSK) Vaccines Institute for Global Health (GVGH) invasive nontyphoidal Salmonella-typhoid conjugate (iNTS-TCV) candidate vaccine to be administered for the first time in humans. The study intervention will be evaluated in European adults in Stage 1 (a 2-step staggered design) followed by African adults in Stage 2.

Detailed Description

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Conditions

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Salmonella Infections

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

SEQUENTIAL

Primary Study Purpose

PREVENTION

Blinding Strategy

TRIPLE

Participants Investigators Outcome Assessors
Data will be collected in an observer-blind manner.

Study Groups

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Stage 1: Invasive nontyphoidal Salmonella (iNTS)-Typhoid conjugate vaccine (TCV) low dose group

European participants were randomized to receive 3 doses of iNTS-TCV low dose vaccine and 3 doses of saline solution intramuscularly, in different arms, on Day 1, Day 57 and Day 169.

Group Type EXPERIMENTAL

Invasive nontyphoidal Salmonella-typhoid conjugate vaccine (iNTS-TCV) low dose

Intervention Type BIOLOGICAL

3 doses of iNTS-TCV low dose vaccine administered intramuscularly at Day 1, Day 57, and Day 169 to participants in the iNTS-TCV low dose Group in Stage 1 (Europe).

Saline

Intervention Type OTHER

3 doses of saline solution administered intramuscularly at Day 1, Day 57, and Day 169 to the participants.

Stage 1: iNTS-Generalized modules for membrane antigens (GMMA) + TCV low dose group

European participants were randomized to receive 3 doses of iNTS-GMMA low dose vaccine and 3 doses of TCV low dose vaccine intramuscularly, in different arms, on Day 1, Day 57 and Day 169.

Group Type ACTIVE_COMPARATOR

Invasive nontyphoidal Salmonella-generalized modules for membrane antigens vaccine (iNTS-GMMA) low dose

Intervention Type BIOLOGICAL

3 doses of iNTS-GMMA low dose vaccine administered intramuscularly at Day 1, Day 57, and Day 169 to participants in the iNTS-GMMA and TCV low doses Group in Stage 1 (Europe).

Typhoid conjugate vaccine (TCV) low dose

Intervention Type BIOLOGICAL

3 doses of TCV low dose vaccine administered intramuscularly at Day 1, Day 57, and Day 169 to participants in the iNTS-GMMA and TCV low doses Group in Stage 1 (Europe).

Stage 1: iNTS-TCV full dose group

European participants were randomized to receive 3 doses of iNTS-TCV full dose vaccine and 3 doses of saline solution intramuscularly, in different arms, on Day 1, Day 57 and Day 169.

Group Type EXPERIMENTAL

Invasive nontyphoidal Salmonella-typhoid conjugate vaccine (iNTS-TCV) full dose

Intervention Type BIOLOGICAL

3 doses of iNTS-TCV full dose vaccine administered intramuscularly at Day 1, Day 57, and Day 169 to participants in the iNTS-TCV full dose Group in Stage 1 (Europe) and to participants in iNTS-TCV full dose Group in Stage 2 (Africa).

Saline

Intervention Type OTHER

3 doses of saline solution administered intramuscularly at Day 1, Day 57, and Day 169 to the participants.

Stage 1: iNTS-GMMA + TCV full dose group

European participants were randomized to receive 3 doses of iNTS-GMMA full dose vaccine and 3 doses of TCV full dose vaccine intramuscularly, in different arms, on Day 1, Day 57 and Day 169.

Group Type ACTIVE_COMPARATOR

Invasive nontyphoidal Salmonella-generalized modules for membrane antigens vaccine (iNTS-GMMA) full dose

Intervention Type BIOLOGICAL

3 doses of iNTS-GMMA full dose vaccine administered intramuscularly at Day 1, Day 57, and Day 169 to participants in the iNTS-GMMA + TCV full dose Group in Stage 1 (Europe) and to participants in the iNTS-GMMA + TCV full dose Group in Stage 2 (Africa).

Typhoid conjugate vaccine (TCV) full dose

Intervention Type BIOLOGICAL

3 doses of TCV full dose vaccine administered intramuscularly at Day 1, Day 57, and Day 169 to participants in the iNTS-GMMA + TCV full dose Group in Stage 1 (Europe) and to participants in the iNTS-GMMA + TCV full dose Group in Stage 2 (Africa).

Stage 1: Placebo group

European participants were randomized to receive 3 doses of Placebo and 3 doses of saline solution intramuscularly, in different arms, on Day 1, Day 57 and Day 169.

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type DRUG

3 doses of Placebo administered intramuscularly at Day 1, Day 57, and Day 169 to participants in the Placebo Group in Stage 1 (Europe).

Saline

Intervention Type OTHER

3 doses of saline solution administered intramuscularly at Day 1, Day 57, and Day 169 to the participants.

Stage 2: iNTS-TCV full dose group

African participants were randomized to receive 3 doses of iNTS-TCV full dose vaccine and 3 doses of saline solution intramuscularly, in different arms, on Day 1, Day 57 and Day 169.

Group Type EXPERIMENTAL

Invasive nontyphoidal Salmonella-typhoid conjugate vaccine (iNTS-TCV) full dose

Intervention Type BIOLOGICAL

3 doses of iNTS-TCV full dose vaccine administered intramuscularly at Day 1, Day 57, and Day 169 to participants in the iNTS-TCV full dose Group in Stage 1 (Europe) and to participants in iNTS-TCV full dose Group in Stage 2 (Africa).

Saline

Intervention Type OTHER

3 doses of saline solution administered intramuscularly at Day 1, Day 57, and Day 169 to the participants.

Stage 2: iNTS-GMMA + TCV full dose group

African participants were randomized to receive 3 doses of iNTS-GMMA full dose vaccine and 3 doses of TCV full dose intramuscularly, in different arms, on Day 1, Day 57 and Day 169.

Group Type ACTIVE_COMPARATOR

Invasive nontyphoidal Salmonella-generalized modules for membrane antigens vaccine (iNTS-GMMA) full dose

Intervention Type BIOLOGICAL

3 doses of iNTS-GMMA full dose vaccine administered intramuscularly at Day 1, Day 57, and Day 169 to participants in the iNTS-GMMA + TCV full dose Group in Stage 1 (Europe) and to participants in the iNTS-GMMA + TCV full dose Group in Stage 2 (Africa).

Typhoid conjugate vaccine (TCV) full dose

Intervention Type BIOLOGICAL

3 doses of TCV full dose vaccine administered intramuscularly at Day 1, Day 57, and Day 169 to participants in the iNTS-GMMA + TCV full dose Group in Stage 1 (Europe) and to participants in the iNTS-GMMA + TCV full dose Group in Stage 2 (Africa).

Stage 2: Control group

African participants were randomized to receive MENVEO as comparator and 1 dose of saline on Day 1, BOOSTRIX as comparator and 1 dose of saline on Day 57 and TYPHIM VI as comparator and 1 dose of saline on Day 169.

Group Type ACTIVE_COMPARATOR

GSK's Meningococcal A, C, Y and W-135 conjugate vaccine

Intervention Type BIOLOGICAL

1 dose of GSK's Meningococcal A, C, Y and W-135 conjugate vaccine administered intramuscularly at Day 1 to participants in the Control Group in Stage 2 (Africa).

GSK's Tetanus toxoid, reduced diphtheria toxoid and acellular pertussis vaccine

Intervention Type COMBINATION_PRODUCT

1 dose of GSK's Tetanus toxoid, reduced diphtheria toxoid and acellular pertussis vaccine administered intramuscularly at Day 57 to participants in the Control Group in Stage 2 (Africa).

Sanofi Pasteur's Typhoid Vi polysaccharide vaccine

Intervention Type COMBINATION_PRODUCT

1 dose of Sanofi Pasteur's Typhoid Vi polysaccharide vaccine administered intramuscularly at Day 169 to participants in the Control Group in Stage 2 (Africa).

Saline

Intervention Type OTHER

3 doses of saline solution administered intramuscularly at Day 1, Day 57, and Day 169 to the participants.

Interventions

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Invasive nontyphoidal Salmonella-typhoid conjugate vaccine (iNTS-TCV) low dose

3 doses of iNTS-TCV low dose vaccine administered intramuscularly at Day 1, Day 57, and Day 169 to participants in the iNTS-TCV low dose Group in Stage 1 (Europe).

Intervention Type BIOLOGICAL

Invasive nontyphoidal Salmonella-generalized modules for membrane antigens vaccine (iNTS-GMMA) low dose

3 doses of iNTS-GMMA low dose vaccine administered intramuscularly at Day 1, Day 57, and Day 169 to participants in the iNTS-GMMA and TCV low doses Group in Stage 1 (Europe).

Intervention Type BIOLOGICAL

Typhoid conjugate vaccine (TCV) low dose

3 doses of TCV low dose vaccine administered intramuscularly at Day 1, Day 57, and Day 169 to participants in the iNTS-GMMA and TCV low doses Group in Stage 1 (Europe).

Intervention Type BIOLOGICAL

Invasive nontyphoidal Salmonella-typhoid conjugate vaccine (iNTS-TCV) full dose

3 doses of iNTS-TCV full dose vaccine administered intramuscularly at Day 1, Day 57, and Day 169 to participants in the iNTS-TCV full dose Group in Stage 1 (Europe) and to participants in iNTS-TCV full dose Group in Stage 2 (Africa).

Intervention Type BIOLOGICAL

Invasive nontyphoidal Salmonella-generalized modules for membrane antigens vaccine (iNTS-GMMA) full dose

3 doses of iNTS-GMMA full dose vaccine administered intramuscularly at Day 1, Day 57, and Day 169 to participants in the iNTS-GMMA + TCV full dose Group in Stage 1 (Europe) and to participants in the iNTS-GMMA + TCV full dose Group in Stage 2 (Africa).

Intervention Type BIOLOGICAL

Typhoid conjugate vaccine (TCV) full dose

3 doses of TCV full dose vaccine administered intramuscularly at Day 1, Day 57, and Day 169 to participants in the iNTS-GMMA + TCV full dose Group in Stage 1 (Europe) and to participants in the iNTS-GMMA + TCV full dose Group in Stage 2 (Africa).

Intervention Type BIOLOGICAL

GSK's Meningococcal A, C, Y and W-135 conjugate vaccine

1 dose of GSK's Meningococcal A, C, Y and W-135 conjugate vaccine administered intramuscularly at Day 1 to participants in the Control Group in Stage 2 (Africa).

Intervention Type BIOLOGICAL

GSK's Tetanus toxoid, reduced diphtheria toxoid and acellular pertussis vaccine

1 dose of GSK's Tetanus toxoid, reduced diphtheria toxoid and acellular pertussis vaccine administered intramuscularly at Day 57 to participants in the Control Group in Stage 2 (Africa).

Intervention Type COMBINATION_PRODUCT

Sanofi Pasteur's Typhoid Vi polysaccharide vaccine

1 dose of Sanofi Pasteur's Typhoid Vi polysaccharide vaccine administered intramuscularly at Day 169 to participants in the Control Group in Stage 2 (Africa).

Intervention Type COMBINATION_PRODUCT

Placebo

3 doses of Placebo administered intramuscularly at Day 1, Day 57, and Day 169 to participants in the Placebo Group in Stage 1 (Europe).

Intervention Type DRUG

Saline

3 doses of saline solution administered intramuscularly at Day 1, Day 57, and Day 169 to the participants.

Intervention Type OTHER

Other Intervention Names

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MENVEO Boostrix TYPHIM Vi

Eligibility Criteria

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Inclusion Criteria

* Participants, who, in the opinion of the Investigator, can and will comply with the requirements of the protocol.
* Written or witnessed/thumb printed informed consent obtained from the participant prior to performance of any study-specific procedure.
* Healthy participants as established by medical history, clinical examination, and laboratory assessment.
* Participant satisfying screening requirements.
* A male or female between and including 18 and 50 years of age at the time of the first study intervention administration.
* Female participants of nonchildbearing potential may be enrolled in the study. Nonchildbearing potential is defined as pre-menarche, current bilateral tubal ligation or occlusion, hysterectomy, bilateral ovariectomy, or post-menopause.
* Female participants of childbearing potential may be enrolled in the trial if the participant:
* Has practiced adequate contraception for 1 month prior to study intervention administration, and
* Has a negative pregnancy test on the day of study intervention administration, and
* Has agreed to continue adequate contraception during the entire treatment period and for 1 month after completion of the study intervention administration series.
* Blood sample for simultaneous follicle-stimulating hormone (FSH) and estradiol levels may be collected at the discretion of the Investigator to confirm non-reproductive potential according to local laboratory reference range.
* Genetic testing for HLA-B27 will be performed at Screening and only participants with a negative result will be allowed to participate in the study\*.

* Only for Stage 1.
* For Malawi (Stage 2), the participant lives in Blantyre and has agreed to remain in Blantyre for the study duration.

Exclusion Criteria

* Known exposure to S. Typhi and nontyphoidal Salmonella confirmed by blood culture during the period starting 3 years prior to first study intervention administration confirmed using past medical history.
* History of any reaction or hypersensitivity associated with any component of the study interventions.
* Any confirmed or suspected immunosuppressive or immunodeficient condition, based on medical history and physical examination.
* Acute or chronic, clinically significant pulmonary, cardiovascular, hepatic, or renal functional abnormality, as determined by physical examination or laboratory screening tests.
* Recurrent history or uncontrolled neurological disorders or seizures.
* Any clinically significant\* hematological and/or biochemical laboratory abnormality.

* The Investigator should use his/her clinical judgment to decide which abnormalities are clinically significant from the panel of tests in the list of safety assays.
* Clinical conditions representing a contraindication to IM injections and/or blood draws.
* Any behavioral or cognitive impairment or psychiatric disease that in the opinion of the Investigator, may interfere with the participant's ability to participate in the study.
* Confirmed positive COVID-19 polymerase chain reaction or lateral flow test during the period starting 28 days before the first administration of study vaccines (Day -28 to Day 1).
* Acute or chronic illness which may be severe enough to preclude participation.
* Any other clinical condition that, in the opinion of the Investigator, might pose additional risk to the participant due to participation in the study.

Prior/Concomitant Therapy

* History of receiving any typhoid vaccine (Ty21a, Vi capsular polysaccharide, or TCV) in the participant's life.
* History of receiving any investigational iNTS or GMMA vaccines in the participant's life.
* Use of any investigational or non-registered product other than the study interventions during the period beginning 30 days (Days -30 to 1) before the first dose of study interventions, or their planned use during the study period.
* A vaccine not foreseen by the study protocol administered during the period starting at 14 days before the first dose and ending 28 days after the last dose of study interventions administration\*, with the exception of flu vaccines or COVID-19 vaccine.

* In case emergency mass vaccination for an unforeseen public health threat (eg, a pandemic) is recommended and/or organized by public health authorities outside the routine immunization program, the time period described above can be reduced if necessary for that vaccine, provided it is used according to the local governmental recommendations and that the Sponsor is notified accordingly.

When regulations allow, the recommended time intervals for administration of these vaccines are at least 7 days before or 7 days after (at least 14 days before or 14 days after in case of live vaccines) each dose of study intervention administration.

* Administration of long-acting immune-modifying drugs at any time during the study period (eg, infliximab).
* Administration of Ig and/or any blood products or plasma derivatives during the period starting 3 months before the administration of the first dose of study interventions or planned administration during the study period.
* Chronic administration (defined as more than 14 days in total) of immunosuppressants or other immune modifying drugs during the period starting 3 months prior to the first study intervention dose(s). For corticosteroids, this will mean prednisone equivalent ≥20 mg/day for adult participants. Inhaled and topical steroids are allowed.

Prior/Concurrent Clinical Study Experience

\- Concurrently participating in another clinical trial, at any time during the study period, in which the participant has been or will be exposed to an investigational or a non-investigational intervention (vaccine and drug).

Other Exclusions

* Pregnant or lactating female
* Female planning to become pregnant or planning to discontinue contraceptive precautions
* History of/current chronic alcohol consumption and/or drug abuse. This will be decided at the discretion of the Investigator. Chronic alcohol consumption is defined as one or more of the following:
* A prolonged period of frequent and heavy alcohol use
* The inability to control drinking once it has begun
* Physical dependence manifested by withdrawal symptoms when the individual stops using alcohol
* Tolerance or the need to use increasing amounts of alcohol to achieve the same effects
* A variety of social and/or legal problems arising from alcohol use.
* Any study personnel or their immediate dependents, family, or household members.
Minimum Eligible Age

18 Years

Maximum Eligible Age

50 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Biomedical Advanced Research and Development Authority

FED

Sponsor Role collaborator

Wellcome Trust

OTHER

Sponsor Role collaborator

Global Antimicrobial Resistance Innovation Fund-(GAMRIF)

UNKNOWN

Sponsor Role collaborator

Bill and Melinda Gates Foundation

OTHER

Sponsor Role collaborator

GlaxoSmithKline

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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GSK Clinical Trials

Role: STUDY_DIRECTOR

GlaxoSmithKline

Locations

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GSK Investigational Site

Edegem, , Belgium

Site Status

GSK Investigational Site

Blantyre, , Malawi

Site Status

Countries

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Belgium Malawi

Provided Documents

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Document Type: Study Protocol

View Document

Document Type: Statistical Analysis Plan

View Document

Other Identifiers

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2021-005178-25

Identifier Type: EUDRACT_NUMBER

Identifier Source: secondary_id

216152

Identifier Type: -

Identifier Source: org_study_id

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