Vitamin D Levels in Umbilical Cord and It's Impact on Infant and Children's Health (VITADi Study)

NCT ID: NCT05419973

Last Updated: 2022-06-30

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

100 participants

Study Classification

OBSERVATIONAL

Study Start Date

2022-05-15

Study Completion Date

2023-03-31

Brief Summary

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This study is a cohort study that aims to assess the relationship between Vitamin D Levels in infant and the risk of anemia, atopic dermatitis, diarrhea and cardiovascular disorders in newborn. The subjects of this study are 100 women who met the inclusion criteria, which are in their third trimester of pregnancy and agree to be included in this study. These subjects then will be examined for their weight using standardized CEBA digital scale, height, upper arm circumference, and blood sampling will also be done to these women to measure their vitamin D levels, calcium, parathyroid hormone, ferritin, Fe serum, IL-16, IL-10. When subjects give birth, the babies will also be included in this study. Anthropometric examination will also be done on the babies, to measure their birth weight, birth length, and head circumference. Blood samples will also be taken from their umbilical cord to assess their complete blood count, ferritin, Fe serum, vitamin D levels, vitamin D receptors, IL-6 and IL-10. All subjects then will be monitored, and home visits will be done when the babies reach 3 months old and 6 months old. An assessment of the incidence of diarrhea, atopic dermatitis, and an assessment of cardiovascular disorders (checking pulse and blood pressure) will be carried out during the visit. At the end of the study, venous blood sampling will be taken to see levels of vitamin D, calcium, parathyroid hormone, routine complete blood count, serum Fe, and ferritin, then data analysis is performed.

Detailed Description

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Participants of this study are women in the third semester of pregnancy, who consented to be included with their babies in this study. Firstly, the parents will be given explanation regarding the study, and informed consent will be signed if they agree to participate. All the pregnant women who met the criteria will be recorded for their name, age, parity, birth and medical history. Anthropometric examination will be done on the pregnant women, to measure weight using standardized CEBA digital scale, height and upper arm circumference. Blood sampling will also be done to measure complete blood count, ferritin, Fe serum, vitamin D levels, calcium, parathyroid hormone, IL-6 and IL-10.

After the subjects give birth, the babies who met the criteria (aterm, birth weight \> 2500 grams, with no congenital abnormalities and acute infection at birth) will also be included in this study. Measurement of birth weight, birth length, and head circumference will be carried out along with blood sampling by taking 3 ml of blood from umbilical cord, 1,5 ml each to measure the complete blood count, and other 1,5 ml to measure ferritin, Fe serum, vitamin D levels, vitamin D receptors, IL-6 and IL-10. Subjects will be monitored for 6 months, and two home visits will be done throughout the study at the age of 3 months and 6 months. During the visits, subjects will be measured for their weight, length, head circumference, as well as their nutritional status. Parents and guardians will also be asked questions regarding the incidence of diarrhea, allergic manifestations such as rash, and assessment will be made based on Hanifin Rajka criteria. At the age of 6 months old, blood sampling will be done again by taking 3 ml of the blood, 1,5 ml each to measure the complete blood count, and other 1,5 ml to measure ferritin, Fe serum, vitamin D levels, vitamin D receptors, IL-6 and IL-10.

The collected data then will be grouped based on the purpose and type of data, then univariate analysis and bivariate analysis will be performed. Univariate analysis will describe the characteristics of the basic data, in the form of frequency, mean value, standard deviation, and range. Meanwhile bivariate analysis such as Wilcoxon test will be performed to to analyse data with independent variables with an ordinal/numeric scale, the data are not normally distributed and have different variances. Mann-Whitney test will also be performed test to compare ordinal or numeric scale variables whose data are not normally distributed and have different variances between two unpaired groups.

Conditions

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Vitamin D Deficiency

Keywords

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Vitamin D in Infants

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Pregnant Women

Women in third semester of pregnancy who are willing to include their babies as samples for the next study, no history of abortion, and not suffering from hypertension and preeclampsia.

Anthropometric measurement for pregnant women

Intervention Type OTHER

Weight, length, upper arm circumference

Blood sampling

Intervention Type OTHER

3 ml of blood will be taken, 1,5 ml each to measure the complete blood count, and other 1,5 ml to measure ferritin, Fe serum, vitamin D levels, vitamin D receptors, IL-6 and IL-10.

Infants

Babies born from mothers who are willing to be included in the study, aterm, with birth weight more than 2500 grams. No congenital abnormalities at birth and no acute infection at birth.

Anthropometric measurement for infants

Intervention Type OTHER

birth weight, length, head circumference, nutritional status

Blood sampling

Intervention Type OTHER

3 ml of blood will be taken, 1,5 ml each to measure the complete blood count, and other 1,5 ml to measure ferritin, Fe serum, vitamin D levels, vitamin D receptors, IL-6 and IL-10.

Interventions

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Anthropometric measurement for pregnant women

Weight, length, upper arm circumference

Intervention Type OTHER

Anthropometric measurement for infants

birth weight, length, head circumference, nutritional status

Intervention Type OTHER

Blood sampling

3 ml of blood will be taken, 1,5 ml each to measure the complete blood count, and other 1,5 ml to measure ferritin, Fe serum, vitamin D levels, vitamin D receptors, IL-6 and IL-10.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Women in third trimester of pregnancy
* Agree to include their babies in this study

Exclusion Criteria

* History of abortion
* Suffering from hypertension or preeclampsia
Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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Hasanuddin University

OTHER

Sponsor Role lead

Responsible Party

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dr. Sri Hardiyanti Putri, Sp.A

Principal Investigator, Pediatrician, Teaching Staff at Pediatric Department Hasanuddin University

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Sri Hardiyanti Putri, MD

Role: PRINCIPAL_INVESTIGATOR

Hasanuddin University

Locations

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Hasanuddin University

Makassar, South Sulawesi, Indonesia

Site Status RECRUITING

Countries

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Indonesia

Central Contacts

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Sri Hardiyanti Putri, MD

Role: CONTACT

Phone: 628114499339

Email: [email protected]

Bahrul Fikry, MD, PhD

Role: CONTACT

Phone: 6282123997492

Email: [email protected]

Facility Contacts

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Sri Hardiyanti Putri, MD

Role: primary

Bahrul Fikri, MD, PhD

Role: backup

Provided Documents

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Document Type: Study Protocol and Statistical Analysis Plan

View Document

Related Links

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https://doi.org/10.1016/j.jpeds.2013.08.060

Atkinson, MA, Melamed, ML, Kumar, J., Roy, CN, Miller, ER, 3rd, Furth, SL, \& Fadrowski, JJ (2014). Vitamin D, race, and risk for anemia in children. The Journal of pediatrics, 164(1), 153-158.e1.

https://doi.org/10.1186/1824-7288-39-35

Cadario, F., Savastio, S., Pozzi, E., Capelli, A., Dondi, E., Gatto, M., Zaffaroni, M., \& Bona, G. (2013). Vitamin D status in cord blood and newborns: Ethnic differences. Italian Journal of Pediatrics, 39(1).

https://doi.org/10.3389/fimmu.2019.02739

Cyprian, F., Lefkou, E., Varoudi, K., \& Girardi, G. (2019). Immunomodulatory Effects of Vitamin D in Pregnancy and Beyond. Frontiers in Immunology, 10.

Other Identifiers

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UH22030108

Identifier Type: -

Identifier Source: org_study_id