Trial Outcomes & Findings for Rapid Blood Culture Identification Panel in Pediatric Patients in Guatemala (NCT NCT05314816)

NCT ID: NCT05314816

Last Updated: 2024-06-14

Results Overview

Calculated from the time of blood culture draw to the time that optimal antimicrobial therapy is started. Optimal antimicrobial therapy is defined based on pre-determined organism-antimicrobial guidelines.

Recruitment status

COMPLETED

Study phase

NA

Target enrollment

479 participants

Primary outcome timeframe

14 days

Results posted on

2024-06-14

Participant Flow

Participant milestones

Participant milestones
Measure
Blood Culture Identification Panel
BioFire BCID2 (Blood culture identification panel) will be performed on positive blood culture specimens. Blood culture identification panel: multiplex PCR panel to be performed on positive blood cultures
Concurrent Control
Standard blood culture process will be performed concurrently on select positive blood culture specimens during the study period. BCID2 will NOT be performed on these specimens.
Retrospective Control
Standard blood culture process was performed prior to the study period.
Overall Study
STARTED
159
221
99
Overall Study
Analyzed in Concurrent Control Group
6
0
0
Overall Study
COMPLETED
141
213
98
Overall Study
NOT COMPLETED
18
8
1

Reasons for withdrawal

Reasons for withdrawal
Measure
Blood Culture Identification Panel
BioFire BCID2 (Blood culture identification panel) will be performed on positive blood culture specimens. Blood culture identification panel: multiplex PCR panel to be performed on positive blood cultures
Concurrent Control
Standard blood culture process will be performed concurrently on select positive blood culture specimens during the study period. BCID2 will NOT be performed on these specimens.
Retrospective Control
Standard blood culture process was performed prior to the study period.
Overall Study
Lost to Follow-up
5
0
1
Overall Study
Protocol Violation
13
8
0

Baseline Characteristics

Race and Ethnicity were not collected from any participant.

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Blood Culture Identification Panel
n=135 Participants
BioFire BCID2 (Blood culture identification panel) will be performed on positive blood culture specimens. Blood culture identification panel: multiplex PCR panel to be performed on positive blood cultures
Concurrent Control
n=219 Participants
Standard blood culture process will be performed concurrently on select positive blood culture specimens during the study period. BCID2 will NOT be performed on these specimens.
Retrospective Control
n=98 Participants
Standard blood culture process was performed prior to the study period.
Total
n=452 Participants
Total of all reporting groups
Age, Categorical
<=18 years
135 Participants
n=135 Participants
219 Participants
n=219 Participants
98 Participants
n=98 Participants
452 Participants
n=452 Participants
Age, Categorical
Between 18 and 65 years
0 Participants
n=135 Participants
0 Participants
n=219 Participants
0 Participants
n=98 Participants
0 Participants
n=452 Participants
Age, Categorical
>=65 years
0 Participants
n=135 Participants
0 Participants
n=219 Participants
0 Participants
n=98 Participants
0 Participants
n=452 Participants
Age, Continuous
1 months
n=135 Participants
1 months
n=219 Participants
0 months
n=98 Participants
0.9 months
n=452 Participants
Sex: Female, Male
Female
61 Participants
n=135 Participants
99 Participants
n=219 Participants
36 Participants
n=98 Participants
196 Participants
n=452 Participants
Sex: Female, Male
Male
74 Participants
n=135 Participants
120 Participants
n=219 Participants
62 Participants
n=98 Participants
256 Participants
n=452 Participants
Race and Ethnicity Not Collected
0 Participants
Race and Ethnicity were not collected from any participant.
Region of Enrollment
Guatemala
135 participants
n=135 Participants
219 participants
n=219 Participants
98 participants
n=98 Participants
452 participants
n=452 Participants
Antecedent medical conditions
No antecedent medical conditions
70 Participants
n=135 Participants
114 Participants
n=219 Participants
25 Participants
n=98 Participants
209 Participants
n=452 Participants
Antecedent medical conditions
One or more medical condition
65 Participants
n=135 Participants
105 Participants
n=219 Participants
73 Participants
n=98 Participants
243 Participants
n=452 Participants
Central line
67 Participants
n=135 Participants
132 Participants
n=219 Participants
70 Participants
n=98 Participants
269 Participants
n=452 Participants
ICU stay
68 Participants
n=135 Participants
133 Participants
n=219 Participants
73 Participants
n=98 Participants
274 Participants
n=452 Participants

PRIMARY outcome

Timeframe: 14 days

Population: 452 patients completed the study and were analyzed. Note that 6 patients were originally enrolled in the BCID group, but ultimately analyzed in the concurrent control group as they did not receive the intervention as allocated. These 6 patients crossed over from the BCID group into the concurrent control group for the analysis, leaving 135 in the BCID and 219 in the concurrent control group for the analysis.

Calculated from the time of blood culture draw to the time that optimal antimicrobial therapy is started. Optimal antimicrobial therapy is defined based on pre-determined organism-antimicrobial guidelines.

Outcome measures

Outcome measures
Measure
Blood Culture Identification Panel
n=135 Participants
BioFire BCID2 (Blood culture identification panel) will be performed on positive blood culture specimens. Blood culture identification panel: multiplex PCR panel to be performed on positive blood cultures
Concurrent Control
n=219 Participants
Standard blood culture process will be performed concurrently on select positive blood culture specimens during the study period. BCID2 will NOT be performed on these specimens.
Retrospective Control
n=98 Participants
Standard blood culture process was performed prior to the study period.
Time to Optimal Antimicrobial Therapy
31.1 hours
Interval 0.0 to 150.0
117.7 hours
Interval 0.0 to 248.4
90.0 hours
Interval 4.3 to 158.0

SECONDARY outcome

Timeframe: 14 days

Population: 452 patients completed the study and were analyzed. Note that 6 patients were originally enrolled in the BCID group, but ultimately analyzed in the concurrent control group as they did not receive the intervention as allocated. These 6 patients crossed over from the BCID group into the concurrent control group for the analysis, leaving 135 in the BCID and 219 in the concurrent control group for the analysis.

Calculated from the time of blood culture draw to the time of organism identification.

Outcome measures

Outcome measures
Measure
Blood Culture Identification Panel
n=135 Participants
BioFire BCID2 (Blood culture identification panel) will be performed on positive blood culture specimens. Blood culture identification panel: multiplex PCR panel to be performed on positive blood cultures
Concurrent Control
n=219 Participants
Standard blood culture process will be performed concurrently on select positive blood culture specimens during the study period. BCID2 will NOT be performed on these specimens.
Retrospective Control
n=98 Participants
Standard blood culture process was performed prior to the study period.
Time to Organism Identification
22.6 hours
Interval 20.3 to 28.0
79.8 hours
Interval 61.7 to 104.5
76.8 hours
Interval 66.9 to 91.0

SECONDARY outcome

Timeframe: 14 days

Population: 452 patients completed the study and were analyzed. Note that 6 patients were originally enrolled in the BCID group, but ultimately analyzed in the concurrent control group as they did not receive the intervention as allocated. These 6 patients crossed over from the BCID group into the concurrent control group for the analysis, leaving 135 in the BCID and 219 in the concurrent control group for the analysis.

Calculated from the time of blood culture draw to the time that the effective therapy is started. Effective therapy is determined based on susceptibilities of the organism.

Outcome measures

Outcome measures
Measure
Blood Culture Identification Panel
n=135 Participants
BioFire BCID2 (Blood culture identification panel) will be performed on positive blood culture specimens. Blood culture identification panel: multiplex PCR panel to be performed on positive blood cultures
Concurrent Control
n=219 Participants
Standard blood culture process will be performed concurrently on select positive blood culture specimens during the study period. BCID2 will NOT be performed on these specimens.
Retrospective Control
n=98 Participants
Standard blood culture process was performed prior to the study period.
Time to Effective Antimicrobial Therapy
0 hours
Interval 0.0 to 33.9
0.2 hours
Interval 0.0 to 120.2
3.0 hours
Interval 0.0 to 89.0

SECONDARY outcome

Timeframe: 30 days

Population: 452 patients completed the study and were analyzed. Note that 6 patients were originally enrolled in the BCID group, but ultimately analyzed in the concurrent control group as they did not receive the intervention as allocated. These 6 patients crossed over from the BCID group into the concurrent control group for the analysis, leaving 135 in the BCID and 219 in the concurrent control group for the analysis.

30 day mortality

Outcome measures

Outcome measures
Measure
Blood Culture Identification Panel
n=135 Participants
BioFire BCID2 (Blood culture identification panel) will be performed on positive blood culture specimens. Blood culture identification panel: multiplex PCR panel to be performed on positive blood cultures
Concurrent Control
n=219 Participants
Standard blood culture process will be performed concurrently on select positive blood culture specimens during the study period. BCID2 will NOT be performed on these specimens.
Retrospective Control
n=98 Participants
Standard blood culture process was performed prior to the study period.
All-cause Mortality
14 Participants
30 Participants
18 Participants

SECONDARY outcome

Timeframe: 30 days

Population: 452 patients completed the study and were analyzed. Note that 6 patients were originally enrolled in the BCID group, but ultimately analyzed in the concurrent control group as they did not receive the intervention as allocated. These 6 patients crossed over from the BCID group into the concurrent control group for the analysis, leaving 135 in the BCID and 219 in the concurrent control group for the analysis.

Outcome measures

Outcome measures
Measure
Blood Culture Identification Panel
n=135 Participants
BioFire BCID2 (Blood culture identification panel) will be performed on positive blood culture specimens. Blood culture identification panel: multiplex PCR panel to be performed on positive blood cultures
Concurrent Control
n=219 Participants
Standard blood culture process will be performed concurrently on select positive blood culture specimens during the study period. BCID2 will NOT be performed on these specimens.
Retrospective Control
n=98 Participants
Standard blood culture process was performed prior to the study period.
Length of Hospital Stay
17 days
Interval 11.0 to 30.0
20 days
Interval 11.0 to 30.0
17 days
Interval 10.0 to 30.0

SECONDARY outcome

Timeframe: 30 days

Population: 452 patients completed the study and were analyzed. Note that 6 patients were originally enrolled in the BCID group, but ultimately analyzed in the concurrent control group as they did not receive the intervention as allocated. These 6 patients crossed over from the BCID group into the concurrent control group for the analysis, leaving 135 in the BCID and 219 in the concurrent control group for the analysis.

Days in the ICU

Outcome measures

Outcome measures
Measure
Blood Culture Identification Panel
n=135 Participants
BioFire BCID2 (Blood culture identification panel) will be performed on positive blood culture specimens. Blood culture identification panel: multiplex PCR panel to be performed on positive blood cultures
Concurrent Control
n=219 Participants
Standard blood culture process will be performed concurrently on select positive blood culture specimens during the study period. BCID2 will NOT be performed on these specimens.
Retrospective Control
n=98 Participants
Standard blood culture process was performed prior to the study period.
Intensive Care Unit Days
14.3 days
Interval 7.5 to 26.2
14.8 days
Interval 8.5 to 28.0
9.0 days
Interval 6.0 to 15.5

Adverse Events

Blood Culture Identification Panel

Serious events: 0 serious events
Other events: 0 other events
Deaths: 14 deaths

Concurrent Control

Serious events: 0 serious events
Other events: 0 other events
Deaths: 30 deaths

Retrospective Control

Serious events: 0 serious events
Other events: 0 other events
Deaths: 18 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Dr. Kelly Graff

Medical College of Wisconsin

Phone: 414-266-4123

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place