Clinical Study of Human Umbilical Cord Mesenchymal Stem Cells in the Treatment of Premature Ovarian Insufficiency

NCT ID: NCT05308342

Last Updated: 2022-04-04

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

66 participants

Study Classification

INTERVENTIONAL

Study Start Date

2019-11-20

Study Completion Date

2023-12-01

Brief Summary

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This study was a single-center, randomized, controlled prospective study. Those who had premature ovarian failure and who had fertility requirements were enrolled in the study. To determine the efficacy and safety of umbilical cord mesenchymal stem cells in the treatment of patients with POI.

Detailed Description

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According to the enrollment and exclusion criteria, the patients were enrolled, and the subjects were randomly divided into two groups by computer randomization. Group A was hormone replacement combined with transplantation of umbilical cord mesenchymal stem cells (test group). Group B was the hormone replacement group (control group).

Conditions

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Premature Ovarian Insufficiency

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

TRIPLE

Participants Caregivers Investigators

Study Groups

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UC-MSCs+hormone replacement group

Group A was the hormone replacement combined with transplantation of umbilical cord mesenchymal stem cells group(test group).

Group Type EXPERIMENTAL

transplantation of human UC-MSCs into ovaries of POI patients

Intervention Type PROCEDURE

UC-MSCs (GMP grade, from Clinical Center for Stem Cell Research of the Affiliated Drum Tower Hospital of Nanjing University Medical School, licensed by the China Food and Drug Administration) are injected into the ovary of patients under transvaginal ultrasonographic (TVUS)-guidance. A total number of 10×106 cells, 5×106 for unilateral ovarian injection) is immediately preserved and transferred for direct injection. After vaginal sterilization, TVUS-guided transplantation is performed by two senior-level medical physicians, using a SIEMENS ACUSON ANTANES premium edition system (SIEMENS AG Healthcare Sector, Erlangen, Germany), equipped with a 6-10 MHz probe. The UC-MSC solution is injected into the ovary by using 21-G PTC needles (Hakko Medical Co, Japan) under TVUS guidance.

hormone replacement treatment

Intervention Type DRUG

Routine estrogen progesterone replacement periodic therapy

hormone replacement group

Group B was the hormone replacement group (control group).

Group Type ACTIVE_COMPARATOR

hormone replacement treatment

Intervention Type DRUG

Routine estrogen progesterone replacement periodic therapy

Interventions

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transplantation of human UC-MSCs into ovaries of POI patients

UC-MSCs (GMP grade, from Clinical Center for Stem Cell Research of the Affiliated Drum Tower Hospital of Nanjing University Medical School, licensed by the China Food and Drug Administration) are injected into the ovary of patients under transvaginal ultrasonographic (TVUS)-guidance. A total number of 10×106 cells, 5×106 for unilateral ovarian injection) is immediately preserved and transferred for direct injection. After vaginal sterilization, TVUS-guided transplantation is performed by two senior-level medical physicians, using a SIEMENS ACUSON ANTANES premium edition system (SIEMENS AG Healthcare Sector, Erlangen, Germany), equipped with a 6-10 MHz probe. The UC-MSC solution is injected into the ovary by using 21-G PTC needles (Hakko Medical Co, Japan) under TVUS guidance.

Intervention Type PROCEDURE

hormone replacement treatment

Routine estrogen progesterone replacement periodic therapy

Intervention Type DRUG

Other Intervention Names

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HRT

Eligibility Criteria

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Inclusion Criteria

1. Those who meet the POF diagnostic criteria and have no spontaneous follicular activity;
2. Married, 20 years old ≤ age \< 40 years old;
3. The average diameter of each ovary is \> 10 mm;
4. Have agreed to sign the informed consent form.

Exclusion Criteria

1. Female and/or male chromosomal abnormalities;
2. Endometriosis, adenomyosis;
3. Postoperative ovarian borderline or malignant tumor;
4. Uterine dysplasia;
5. Associated with female autoimmune disease or other serious internal surgical diseases;
6. hormone replacement contraindications;
7. In the past 1 year, had received an experimental study of premature ovarian failure in the external hospital;
8. Male azoospermia or severe oligozoospermia.
Minimum Eligible Age

20 Years

Maximum Eligible Age

39 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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Li-jun Ding

OTHER

Sponsor Role lead

Responsible Party

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Li-jun Ding

principal investigator

Responsibility Role SPONSOR_INVESTIGATOR

Principal Investigators

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Guangshu Han, phD

Role: PRINCIPAL_INVESTIGATOR

The Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School

Locations

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Reproductive Medicine Center, The affiliated Drum Towel Hospital of Nanjing University Medical School

Nanjing, Jiangsu, China

Site Status RECRUITING

Countries

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China

Central Contacts

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Haixiang Sun, phD

Role: CONTACT

+862583107188

Lijun Ding, phD

Role: CONTACT

+862883107188

Facility Contacts

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Haixiang Sun

Role: primary

+86 25 8310 7188

Other Identifiers

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SZ-POF-2019-4

Identifier Type: -

Identifier Source: org_study_id

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