Adipose Tissue Pre and Post Bariatric Surgery

NCT ID: NCT05304325

Last Updated: 2022-03-31

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

98 participants

Study Classification

OBSERVATIONAL

Study Start Date

2012-04-25

Study Completion Date

2022-09-30

Brief Summary

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Published data suggest that inflammation and fibrosis of adipose tissue could be factors favoring the development of insulin resistance in obese individuals and that a decrease in the activity of the AMP-activity kinase protein (AMPK) could lead to these dysfunctions. However, very few data are available in humans.

There is also growing interest in persistent organic pollutants (POPs) as a cardiometabolic and type 2 diabetes (T2D) risk factor. There is some evidence to suggest that POPs directly contribute to lipid metabolism dysfunction and insulin resistance. Additionally, POPs are stocked in adipose tissue. The accumulation of POPs in adipose tissue therefore limits their bioavailability to other organs, thus reducing their systemic toxicity. It has been observed that a large amplitude weight loss leads to a significant increase in POPs in the blood.

The goal of this project is to identify adipose tissue factors/dysfunctions that contribute to insulin resistance and type 2 diabetes associated with obesity in humans and thus raise avenues for screening and treatment of these metabolic complications. More specifically, the objectives are:

* To study the relationship between AMPK, fibrosis and inflammation of adipose tissue and their role in the development of insulin resistance and T2D associated with obesity;
* To examine the relationship between POPs and the cardiometabolic profile.

Detailed Description

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Conditions

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Obesity

Keywords

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Bariatric surgery Persistant organic pollutants Type 2 diabetes

Study Design

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Observational Model Type

OTHER

Study Time Perspective

PROSPECTIVE

Study Groups

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Obese with type 2 diabetes

Bariatric surgery

Intervention Type PROCEDURE

Sleeve gastrectomy

Blood sampling

Intervention Type OTHER

Blood samples will be collected to measure glucose, insulin, albumin, uric acid, HbA1c, lipids, hepatic enzymes, TSH, hs-CRP, complete blood cell counts and persistant organic pollutants

Blood pressure

Intervention Type OTHER

Systolic and diastolic blood pressure will be measured.

Anthropometric measurements

Intervention Type OTHER

Body weight, height and waist circumference will be measured.

Body composition

Intervention Type OTHER

Body composition will be evaluated by dual-energy X-ray absorptiometry.

Resting metabolic rate

Intervention Type OTHER

Resting metabolic rate will be measured by indirect calorimetry.

Food intake

Intervention Type OTHER

Food intake, energy intake and food quality will be evaluated using the food frequence questionnaire.

Adipose tissue biopsy

Intervention Type PROCEDURE

A sample of 3-5g of adipose tissue will be collected under local anesthesia by needle aspiration in the periumbilical region.

Obese insulin-sensitive

Bariatric surgery

Intervention Type PROCEDURE

Sleeve gastrectomy

Blood sampling

Intervention Type OTHER

Blood samples will be collected to measure glucose, insulin, albumin, uric acid, HbA1c, lipids, hepatic enzymes, TSH, hs-CRP, complete blood cell counts and persistant organic pollutants

Blood pressure

Intervention Type OTHER

Systolic and diastolic blood pressure will be measured.

Anthropometric measurements

Intervention Type OTHER

Body weight, height and waist circumference will be measured.

Body composition

Intervention Type OTHER

Body composition will be evaluated by dual-energy X-ray absorptiometry.

Resting metabolic rate

Intervention Type OTHER

Resting metabolic rate will be measured by indirect calorimetry.

Food intake

Intervention Type OTHER

Food intake, energy intake and food quality will be evaluated using the food frequence questionnaire.

Adipose tissue biopsy

Intervention Type PROCEDURE

A sample of 3-5g of adipose tissue will be collected under local anesthesia by needle aspiration in the periumbilical region.

Obese insulin-resistant

Bariatric surgery

Intervention Type PROCEDURE

Sleeve gastrectomy

Blood sampling

Intervention Type OTHER

Blood samples will be collected to measure glucose, insulin, albumin, uric acid, HbA1c, lipids, hepatic enzymes, TSH, hs-CRP, complete blood cell counts and persistant organic pollutants

Blood pressure

Intervention Type OTHER

Systolic and diastolic blood pressure will be measured.

Anthropometric measurements

Intervention Type OTHER

Body weight, height and waist circumference will be measured.

Body composition

Intervention Type OTHER

Body composition will be evaluated by dual-energy X-ray absorptiometry.

Resting metabolic rate

Intervention Type OTHER

Resting metabolic rate will be measured by indirect calorimetry.

Food intake

Intervention Type OTHER

Food intake, energy intake and food quality will be evaluated using the food frequence questionnaire.

Adipose tissue biopsy

Intervention Type PROCEDURE

A sample of 3-5g of adipose tissue will be collected under local anesthesia by needle aspiration in the periumbilical region.

Interventions

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Bariatric surgery

Sleeve gastrectomy

Intervention Type PROCEDURE

Blood sampling

Blood samples will be collected to measure glucose, insulin, albumin, uric acid, HbA1c, lipids, hepatic enzymes, TSH, hs-CRP, complete blood cell counts and persistant organic pollutants

Intervention Type OTHER

Blood pressure

Systolic and diastolic blood pressure will be measured.

Intervention Type OTHER

Anthropometric measurements

Body weight, height and waist circumference will be measured.

Intervention Type OTHER

Body composition

Body composition will be evaluated by dual-energy X-ray absorptiometry.

Intervention Type OTHER

Resting metabolic rate

Resting metabolic rate will be measured by indirect calorimetry.

Intervention Type OTHER

Food intake

Food intake, energy intake and food quality will be evaluated using the food frequence questionnaire.

Intervention Type OTHER

Adipose tissue biopsy

A sample of 3-5g of adipose tissue will be collected under local anesthesia by needle aspiration in the periumbilical region.

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

1. Awaiting bariatric surgery
2. BMI ≥ 35 kg/m2 without cardiometabolic complications or ≥ 30 kg/m2 if presence of type 2 diabetes or hypertension
3. ≥ 18 years old
4. Non smoker
5. Sedentary (less than 3 hours of regular physical activity per week)

Exclusion Criteria

1. Type 1 diabetes
2. Acute event in the last 3 months (myocardial infarction, cerebrovascular hemorrhage or transient cerebral ischemia, unstable angina, peripheral vascular disease, revascularization or angioplasty, recent hospitalization for more than 4 days)
3. Infection in the last month (fever, antibiotics treatment)
4. Cancer in the last 3 years
5. Chronic inflammatory disease
6. Pharmacologic treatment (any medication affecting glucose metabolism (except those for diabetes), hypotensive medication unless stable dose for at least 1 month, lipid-lowering medication unless stable dose for at least 1 month)
7. Uncontrolled disease of pituitary or thyroid gland
8. Bleeding disorders
9. Alcohol or drug abuses
10. Claustrophobia
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Institut de Recherches Cliniques de Montreal

OTHER

Sponsor Role lead

Responsible Party

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Rémi Rabasa-Lhoret

Full professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Rémi Rabasa-Lhoret

Role: PRINCIPAL_INVESTIGATOR

Institut de recherches cliniques de Montréal

Locations

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Institut de recherches cliniques de Montréal

Montreal, Quebec, Canada

Site Status

Countries

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Canada

Other Identifiers

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2011-22

Identifier Type: -

Identifier Source: org_study_id