Trial Outcomes & Findings for Evaluation of an Oral Cannabidiol (CBD)-Terpene Formulation on Sleep Physiology in Participants With Insomnia (NCT NCT05233761)

NCT ID: NCT05233761

Last Updated: 2025-09-11

Results Overview

The primary endpoint is the percentage of time spent in slow wave (Deep) and REM sleep as quantified by a non-invasive wrist-worn sleep-tracking device called Whoop.

Recruitment status

COMPLETED

Study phase

PHASE2

Target enrollment

125 participants

Primary outcome timeframe

Participants were assessed for treatment with CBD-terpenes and placebo, each for a treatment period of four weeks. Participants took the treatment (CBD-terpenes or Placebo) for at least four nights per week over the entire four week treatment period.

Results posted on

2025-09-11

Participant Flow

Participant milestones

Participant milestones
Measure
Group 1 (A to B: A = Placebo; B = Defined CBD)
The placebo control will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that do not contain any CBD or terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks. The Placebo capsules look and smell identical to the Defined CBD capsules. Defined CBD will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that contain 300 mg CBD and 8 mg terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks.
Group 2 (B to A: B = Defined CBD; A = Placebo)
Defined CBD will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that contain 300 mg CBD and 8 mg terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks. The placebo control will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that do not contain any CBD or terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks. The Placebo capsules look and smell identical to the Defined CBD capsules.
Treatment Period A
STARTED
63
62
Treatment Period A
COMPLETED
37
25
Treatment Period A
NOT COMPLETED
26
37
Treatment Period B
STARTED
37
25
Treatment Period B
COMPLETED
37
25
Treatment Period B
NOT COMPLETED
0
0

Reasons for withdrawal

Reasons for withdrawal
Measure
Group 1 (A to B: A = Placebo; B = Defined CBD)
The placebo control will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that do not contain any CBD or terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks. The Placebo capsules look and smell identical to the Defined CBD capsules. Defined CBD will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that contain 300 mg CBD and 8 mg terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks.
Group 2 (B to A: B = Defined CBD; A = Placebo)
Defined CBD will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that contain 300 mg CBD and 8 mg terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks. The placebo control will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that do not contain any CBD or terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks. The Placebo capsules look and smell identical to the Defined CBD capsules.
Treatment Period A
Withdrawal by Subject
12
10
Treatment Period A
Lost to Follow-up
6
12
Treatment Period A
Protocol Violation
8
15

Baseline Characteristics

Race and Ethnicity were not collected from any participant.

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Group 1 (A to B: A = Placebo; B = Defined CBD)
n=63 Participants
The placebo control will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that do not contain any CBD or terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks. The Placebo capsules look and smell identical to the Defined CBD capsules. Defined CBD will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that contain 300 mg CBD and 8 mg terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks.
Group 2 (B to A: B = Defined CBD; A = Placebo)
n=62 Participants
Defined CBD will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that contain 300 mg CBD and 8 mg terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks. The placebo control will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that do not contain any CBD or terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks. The Placebo capsules look and smell identical to the Defined CBD capsules.
Total
n=125 Participants
Total of all reporting groups
Age, Categorical
<=18 years
0 Participants
n=63 Participants
0 Participants
n=62 Participants
0 Participants
n=125 Participants
Age, Categorical
Between 18 and 65 years
63 Participants
n=63 Participants
62 Participants
n=62 Participants
125 Participants
n=125 Participants
Age, Categorical
>=65 years
0 Participants
n=63 Participants
0 Participants
n=62 Participants
0 Participants
n=125 Participants
Sex: Female, Male
Female
51 Participants
n=63 Participants
50 Participants
n=62 Participants
101 Participants
n=125 Participants
Sex: Female, Male
Male
12 Participants
n=63 Participants
12 Participants
n=62 Participants
24 Participants
n=125 Participants
Race and Ethnicity Not Collected
0 Participants
Race and Ethnicity were not collected from any participant.
Region of Enrollment
United States
63 participants
n=63 Participants
62 participants
n=62 Participants
125 participants
n=125 Participants

PRIMARY outcome

Timeframe: Participants were assessed for treatment with CBD-terpenes and placebo, each for a treatment period of four weeks. Participants took the treatment (CBD-terpenes or Placebo) for at least four nights per week over the entire four week treatment period.

Population: Data from six participants \[four participants from Group 1 (A/B) and two participants from Group 2 (B/A)\] were excluded from the final data analysis due to protocol deviations (e.g. due to a lack of reporting whether or not they took the study treatment, and/or insufficient sleep data collection).

The primary endpoint is the percentage of time spent in slow wave (Deep) and REM sleep as quantified by a non-invasive wrist-worn sleep-tracking device called Whoop.

Outcome measures

Outcome measures
Measure
Defined CBD
n=56 Participants
Defined CBD will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that contain a total of 300 mg CBD and 8 mg terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks. Defined CBD: 300 mg CBD and 8 mg Terpenes administered as two small capsules.
Placebo
n=56 Participants
The placebo control will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that do not contain any CBD or terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks. The Placebo capsules will look and smell identical to the Defined CBD capsules. Placebo: Capsules that smell and look exactly like Defined CBD but contain no CBD or terpenes.
Percentage of Time Spent in the Combination of Slow Wave Sleep (SWS) and Rapid Eye Movement (REM) Sleep
43.0 Percentage of total sleep time
Standard Error 0.4
41.8 Percentage of total sleep time
Standard Error 0.4

SECONDARY outcome

Timeframe: Four weeks

Population: Data from six participants \[four participants from Group 1 (A/B) and two participants from Group 2 (B/A)\] were excluded from the final data analysis due to protocol deviations (e.g. due to a lack of reporting whether or not they took the study treatment, and/or insufficient sleep data collection).

The percentage of time spent in each sleep stage (SWS, REM, light and awake) relative to total sleep time as quantified by a non-invasive wrist-worn sleep-tracking device called Whoop.

Outcome measures

Outcome measures
Measure
Defined CBD
n=56 Participants
Defined CBD will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that contain a total of 300 mg CBD and 8 mg terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks. Defined CBD: 300 mg CBD and 8 mg Terpenes administered as two small capsules.
Placebo
n=56 Participants
The placebo control will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that do not contain any CBD or terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks. The Placebo capsules will look and smell identical to the Defined CBD capsules. Placebo: Capsules that smell and look exactly like Defined CBD but contain no CBD or terpenes.
Objective Secondary Outcome Measures of Sleep Physiology
SWS Sleep (%TST)
19.1 Percentage of TST
Standard Error 0.2
18.1 Percentage of TST
Standard Error 0.2
Objective Secondary Outcome Measures of Sleep Physiology
REM Sleep (% TST)
24.0 Percentage of TST
Standard Error 0.3
23.7 Percentage of TST
Standard Error 0.3
Objective Secondary Outcome Measures of Sleep Physiology
Light Sleep (% TST)
56.0 Percentage of TST
Standard Error 0.4
57.7 Percentage of TST
Standard Error 0.4
Objective Secondary Outcome Measures of Sleep Physiology
Awake Time (% TST)
22.4 Percentage of TST
Standard Error 0.5
21.7 Percentage of TST
Standard Error 0.4

SECONDARY outcome

Timeframe: Four weeks

Population: Data from six participants \[four participants from Group 1 (A/B) and two participants from Group 2 (B/A)\] were excluded from the final data analysis due to protocol deviations (e.g. due to a lack of reporting whether or not they took the study treatment, and/or insufficient sleep data collection).

The absolute time spent in each sleep stage (SWS, REM, light and awake) as quantified by a non-invasive wrist-worn sleep-tracking device called Whoop.

Outcome measures

Outcome measures
Measure
Defined CBD
n=56 Participants
Defined CBD will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that contain a total of 300 mg CBD and 8 mg terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks. Defined CBD: 300 mg CBD and 8 mg Terpenes administered as two small capsules.
Placebo
n=56 Participants
The placebo control will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that do not contain any CBD or terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks. The Placebo capsules will look and smell identical to the Defined CBD capsules. Placebo: Capsules that smell and look exactly like Defined CBD but contain no CBD or terpenes.
Objective Secondary Outcome Measures of Sleep Physiology
SWS+REM Sleep (min)
162.5 Min
Standard Error 2.0
155.0 Min
Standard Error 2.2
Objective Secondary Outcome Measures of Sleep Physiology
SWS Sleep (min)
71.1 Min
Standard Error 0.9
67.7 Min
Standard Error 1.0
Objective Secondary Outcome Measures of Sleep Physiology
REM Sleep (min)
91.4 Min
Standard Error 1.4
90.0 Min
Standard Error 1.5
Objective Secondary Outcome Measures of Sleep Physiology
Light Sleep (min)
206.2 Min
Standard Error 2.5
212.9 Min
Standard Error 2.6
Objective Secondary Outcome Measures of Sleep Physiology
Awake Time (min)
80.4 Min
Standard Error 1.7
78.2 Min
Standard Error 1.7
Objective Secondary Outcome Measures of Sleep Physiology
Total Sleep Time (min)
366.5 Min
Standard Error 3.3
373.4 Min
Standard Error 3.3

SECONDARY outcome

Timeframe: Participants were assessed for treatment with CBD-terpenes and placebo, each for a treatment period of four weeks. Participants took the treatment (CBD-terpenes or Placebo) for at least four nights per week over the entire four week treatment period.

Population: Data from six participants \[four participants from Group 1 (A/B) and two participants from Group 2 (B/A)\] were excluded from the final data analysis due to protocol deviations (e.g. due to a lack of reporting whether or not they took the study treatment, and/or insufficient sleep data collection).

A modified version of a clinically validated questionnaire/survey entitled the patient's global impression (PGI) was used to assess subjective perceptions of sleep by the study participants after each four-week treatment period. The modified PGI is a five-item, subjective, participant self-report that assesses treatment benefit to sleep quality (Item 1), sleep induction (Item 2), sleep duration (Item 3), sleep depth (Item 4) and treatment effect on the presence of vivid dreams (item 5). Each item in our modified PGI was presented as a survey that consisted of a ten-point categorical scale, with scores between 6-10 representing a treatment benefit/advantage, a score of 5 representing no effect of treatment, and a score of 1-4 representing a treatment worsening/disadvantage.

Outcome measures

Outcome measures
Measure
Defined CBD
n=56 Participants
Defined CBD will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that contain a total of 300 mg CBD and 8 mg terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks. Defined CBD: 300 mg CBD and 8 mg Terpenes administered as two small capsules.
Placebo
n=56 Participants
The placebo control will be administered as two small capsules that will be taken orally with a glass of water one hour before bedtime that do not contain any CBD or terpenes. Participants will take the treatment on a minimum of four nights per week, over a total of four-weeks. The Placebo capsules will look and smell identical to the Defined CBD capsules. Placebo: Capsules that smell and look exactly like Defined CBD but contain no CBD or terpenes.
Subjective Secondary Outcome Measure of Sleep Physiology
Sleep Better
5.9 Units on a scale
Standard Error 0.4
5.6 Units on a scale
Standard Error 0.4
Subjective Secondary Outcome Measure of Sleep Physiology
Sleep Induction
5.6 Units on a scale
Standard Error 0.4
5.1 Units on a scale
Standard Error 0.4
Subjective Secondary Outcome Measure of Sleep Physiology
Sleep Duration
5.6 Units on a scale
Standard Error 0.4
4.8 Units on a scale
Standard Error 0.4
Subjective Secondary Outcome Measure of Sleep Physiology
Sleep Depth
5.5 Units on a scale
Standard Error 0.4
5.0 Units on a scale
Standard Error 0.4
Subjective Secondary Outcome Measure of Sleep Physiology
Vivid Dreams
4.5 Units on a scale
Standard Error 0.5
4.0 Units on a scale
Standard Error 0.5

Adverse Events

Defined CBD

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Placebo

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Paul Muchowski

Defined Research Institute

Phone: 415-413-8666

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place