The Role of Long Non-coding RNAs WRAP53 and UCA-1 as Potential Biomarkers in Diagnosis of Hepatocellular Carcinoma

NCT ID: NCT05088811

Last Updated: 2021-10-22

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

80 participants

Study Classification

OBSERVATIONAL

Study Start Date

2021-08-21

Study Completion Date

2022-09-30

Brief Summary

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The aim of this work is to study the role of long non-coding RNAs WRAP53 and urothelial carcinoma-associated 1 (UCA1) as potential biomarkers in diagnosis of hepatocellular carcinoma.

Detailed Description

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Sample size calculation was done in department of medical statistics, Medical Research Institute, Alexandria University and the total minimal sample size was calculated.

A prospective case control study will be conducted on 80 subjects divided into three groups:

Group (1): Thirty patients having liver cirrhosis and hepatocellular carcinoma diagnosed by tripahsic CT.

Group (2): Thirty liver cirrhosis patients without hepatocellular carcinoma diagnosed by abdominal ultrasound examination.

Group (3): Twenty normal subjects (control group) matching in age and sex with patients in groups 1 and 2.

Patients will be recruited from the outpatient clinics and inpatient wards of Alexandria Main University Hospital and Medical Research Institute Hospital. Full informed consent will be taken from the patients and approval of the ethical committee of the Faculty of Medicine will be fulfilled. Exclusion criteria: Patients with any inflammatory diseases or other malignancies.

Conditions

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Hepatocellular Carcinoma Liver Cirrhosis Diagnoses Disease

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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Liver cirrhosis

Group (1): Thirty patients having liver cirrhosis and hepatocellular carcinoma diagnosed by tripahsic CT.

WRAP53, UCA-1

Intervention Type DIAGNOSTIC_TEST

Diagnostic value of UCA-1 and WRAP53 in HCC

Hepatocellular Carcinoma

Group (2): Thirty liver cirrhosis patients without hepatocellular carcinoma diagnosed by abdominal ultrasound examination

WRAP53, UCA-1

Intervention Type DIAGNOSTIC_TEST

Diagnostic value of UCA-1 and WRAP53 in HCC

Control

Group (3): Twenty normal subjects (control group) matching in age and sex with patients in groups 1 and 2.

WRAP53, UCA-1

Intervention Type DIAGNOSTIC_TEST

Diagnostic value of UCA-1 and WRAP53 in HCC

Interventions

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WRAP53, UCA-1

Diagnostic value of UCA-1 and WRAP53 in HCC

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Exclusion Criteria

* Patients with any inflammatory diseases or other malignancies.
Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Alexandria University

OTHER

Sponsor Role lead

Responsible Party

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Elsheaita, Ahmed Mohamed Elmoughazy Abdelfatah Aly

Assistant lecturer, experimental and clinical internal medicine, medical research institute

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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Alexandria University, faculty of medicine

Alexandria, , Egypt

Site Status RECRUITING

Alexandria University, medical research institute

Alexandria, , Egypt

Site Status RECRUITING

Countries

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Egypt

Central Contacts

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Ahmed Elsheaita, Msc

Role: CONTACT

+201062099085

Facility Contacts

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Amira Eid, MD

Role: primary

Amal Ghanem, MD

Role: primary

References

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Kamel MM, Matboli M, Sallam M, Montasser IF, Saad AS, El-Tawdi AHF. Investigation of long noncoding RNAs expression profile as potential serum biomarkers in patients with hepatocellular carcinoma. Transl Res. 2016 Feb;168:134-145. doi: 10.1016/j.trsl.2015.10.002. Epub 2015 Oct 24.

Reference Type RESULT
PMID: 26551349 (View on PubMed)

Pouladi N, Abdolahi S, Farajzadeh D, Hosseinpour Feizi MA. Haplotype and linkage disequilibrium of TP53-WRAP53 locus in Iranian-Azeri women with breast cancer. PLoS One. 2019 Aug 6;14(8):e0220727. doi: 10.1371/journal.pone.0220727. eCollection 2019.

Reference Type RESULT
PMID: 31387111 (View on PubMed)

Wang F, Ying HQ, He BS, Pan YQ, Deng QW, Sun HL, Chen J, Liu X, Wang SK. Upregulated lncRNA-UCA1 contributes to progression of hepatocellular carcinoma through inhibition of miR-216b and activation of FGFR1/ERK signaling pathway. Oncotarget. 2015 Apr 10;6(10):7899-917. doi: 10.18632/oncotarget.3219.

Reference Type RESULT
PMID: 25760077 (View on PubMed)

Abdelmoety AA, Elhassafy MY, Said RSO, Elsheaita A, Mahmoud MM. The role of UCA1 and WRAP53 in diagnosis of hepatocellular carcinoma: A single-center case-control study. Clin Exp Hepatol. 2023 Jun;9(2):129-137. doi: 10.5114/ceh.2023.127569. Epub 2023 Jun 18.

Reference Type DERIVED
PMID: 37502440 (View on PubMed)

Other Identifiers

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HCC medical research institute

Identifier Type: -

Identifier Source: org_study_id