Screening Algorithms for Cervical and Anal High-Grade Squamous Intraepithelial Lesions in People With HIV in Mexico and Puerto Rico

NCT ID: NCT05074264

Last Updated: 2025-11-10

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

ACTIVE_NOT_RECRUITING

Clinical Phase

NA

Total Enrollment

1586 participants

Study Classification

INTERVENTIONAL

Study Start Date

2021-11-30

Study Completion Date

2027-12-31

Brief Summary

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This clinical trial aims to find what different tests work best to find high-grade squamous intraepithelial lesions (HSIL) in the cervix or anus in patients living with human immunodeficiency virus (HIV). Patients with HIV are at high risk of becoming infected with human papillomavirus (HPV) in the cervix or anus where it can turn into cancer over several years. HPV causes changes to the cervix and anus, known as HSIL. This means that there is an area of abnormal tissue on the top layers of the cervix or anus. It is considered cervical or anal cancer if the abnormality spreads down into the layers of tissue below the top. If found early, many cases of HSIL can be treated before turning into cancer. Screening for cervical or anal cancer detection or HSIL associated with HPV may result in earlier treatment, if necessary, for patients living with HIV.

Detailed Description

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PRIMARY OBJECTIVE:

I. Evaluate the most efficient algorithm combining point-of-care (POC) HPV-based screening with several triage alternatives: cytology, E6 and/or E7 oncoprotein detection and S5 methylation, to improve the current screening program for detection of anogenital HPV-related cancers and pre-cancers, in men and women living with HIV in Mexico and Puerto Rico.

EXPLORATORY OBJECTIVE:

I. Evaluate artificial intelligence-based algorithms based on cell-phone images of the cervix for their sensitivity, specificity, and negative and positive predictive values for cervical cancers and precancers in women living with HIV in Mexico and Puerto Rico.

II. Evaluate the HPV agreement/concordance between cervicovaginal samples collected by participants and cervical samples collected by clinicians.

OUTLINE: Patients receive standard medical procedures based on anatomy at time of enrollment.

GROUP I (WOMEN LIVING WITH HIV (WLWH)): Patients undergo collection of cervical images, 3 cervical anal swabs and 3 anal swabs for real-time testing of high-risk (hr) HPV over 90 minutes. Patients with a positive hrHPV test on their cervical swab undergo colposcopy and biopsies of visible lesions. Patients with a negative hrHPV test on their cervical swab may undergo a colposcopy. Patients with a positive hrHPV test on their anal swab undergo high-resolution anoscopy at a later visit within 1 month. Patients with a negative hrHPV on their anal swabs may undergo a high-resolution anoscopy and biopsies of visible lesions, and those with a positive anal cytology for low-grade squamous intraepithelial lesion (LSIL) or worse undergo a high-resolution anoscopy and biopsies within 1 month. Patients may be given a diagnosis and treatment at the second visit. Patients diagnosed with HSIL may undergo standard of care (SOC) treatment or enroll in additional studies when they are open to accrual.

GROUP II (MEN LIVING WITH HIV (MLWH)): Patients undergo collection of 3 anal swabs for real-time testing of high-risk HPV over 90 minutes. Patients with a positive hrHPV test undergo high-resolution anoscopy with biopsies of visible lesions. Patients with hrHPV negative test may undergo high-resolution anoscopy. Patients may be given a diagnosis and treatment at the second visit. Patients diagnosed with HSIL may undergo SOC treatment or enroll in additional studies when they are open to accrual.

Conditions

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AIDS-Related Anal Carcinoma AIDS-Related Cervical Carcinoma High Grade Anal Intraepithelial Neoplasia High Grade Cervical Intraepithelial Neoplasia HIV Infection

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

SINGLE_GROUP

Primary Study Purpose

SCREENING

Blinding Strategy

NONE

Study Groups

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Imaging, biospecimen collection, anoscopy and/or colposcopy

Patients undergo collection of cervical images (if applicable), 3 cervical anal swabs (if applicable) and 3 anal swabs for real-time testing of HPV or hrHPV over 90 minutes. Patients with a positive HPV or hrHPV test on undergo biopsies of visible lesions. Patients with a negative hrHPV test on their cervical swab may undergo a colposcopy. Patients with a positive hrHPV test on their anal swab undergo high-resolution anoscopy at a later visit within 1 month. Patients with a negative hrHPV on their anal swabs may undergo a high-resolution anoscopy and biopsies of visible lesions, and those with a positive anal cytology for LSIL or worse undergo a high-resolution anoscopy and biopsies within 1 month. Patients may be given a diagnosis and treatment at the second visit. Patients diagnosed with HSIL may undergo SOC treatment or enroll in additional studies when they are open to accrual

Group Type EXPERIMENTAL

Biopsy

Intervention Type PROCEDURE

Undergo biopsy

Biospecimen Collection - Anal

Intervention Type PROCEDURE

Undergo collection of anal swabs

Biospecimen Collection - Cervical

Intervention Type PROCEDURE

Undergo collection of cervical swabs

Colposcopy

Intervention Type PROCEDURE

Undergo colposcopy

High Resolution Anoscopy

Intervention Type PROCEDURE

Undergo high-resolution anoscopy

Imaging Technique

Intervention Type PROCEDURE

Undergo cervical imaging

Interventions

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Biopsy

Undergo biopsy

Intervention Type PROCEDURE

Biospecimen Collection - Anal

Undergo collection of anal swabs

Intervention Type PROCEDURE

Biospecimen Collection - Cervical

Undergo collection of cervical swabs

Intervention Type PROCEDURE

Colposcopy

Undergo colposcopy

Intervention Type PROCEDURE

High Resolution Anoscopy

Undergo high-resolution anoscopy

Intervention Type PROCEDURE

Imaging Technique

Undergo cervical imaging

Intervention Type PROCEDURE

Other Intervention Names

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Biological Sample Collection Biospecimen Collection Biological Sample Collection Biospecimen Collection HRA Diagnostic Imaging Technique Imaging Imaging Procedures Medical Imaging

Eligibility Criteria

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Inclusion Criteria

* Documentation of HIV-1 infection by means of any one of the following:

* Documentation of HIV diagnosis in the medical record by a licensed health care provider
* Documentation of receipt of antiretroviral therapy (ART) by a licensed health care provider (Documentation may be a record of an ART prescription in the participant's medical record, a written prescription in the name of the participant for ART, or pill bottles for ART with a label showing the participant's name. Receipt of at least two agents is required; each component agent of a multi-class combination ART regimen will be counted toward the 2-agent requirement, excepting receipt of a pre-exposure prophylaxis (PrEP) regimen alone \[e.g., Truvada\], which is exclusionary);
* HIV-1 ribonucleic acid (RNA) detection by a licensed HIV-1 RNA assay demonstrating \> 1000 RNA copies/mL
* Any locally licensed HIV screening antibody and/or HIV antibody/antigen combination assay confirmed by a second licensed HIV assay such as a HIV-1 Western blot confirmation or HIV rapid multispot antibody differentiation assay
* NOTE: A "licensed" assay refers to a United States (U.S.) Food and Drug Administration (FDA)-approved assay or an assay approved by the relevant local health authority
* Age 21 years or older. Cervical HSIL/cancer screening does not usually begin until 20 years of age or older. Also, anal HSIL/cancer screening among high risk individuals such as people living with HIV is recommended for those 25 years of age or older. Children under the age of 18 are at low risk of developing cervical or anal HSIL/cancer and will not benefit from the kind of screening planned for this study
* Eastern Cooperative Oncology Group (ECOG) performance status =\< 1 (Karnofsky score \>= 70%)
* Ability to understand and the willingness to sign a written informed consent document

Exclusion Criteria

* Participants who have undergone hysterectomy
* History of anal cancer, penile, vulvar, vaginal, or cervical cancer
* Potential participants who received prior treatment of anal, cervical, penile, vaginal, or vulvar lesions within 18 months of study enrollment
* Inability in the opinion of the study investigator of the participant to comply with study requirements
* Participants who are pregnant (a urine pregnancy test will be provided to participants aged 60 years or less) or within 2 months being post-partum
Minimum Eligible Age

21 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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National Cancer Institute (NCI)

NIH

Sponsor Role collaborator

US-Latin American-Caribbean HIV/HPV-Cancer Prevention Clinical Trials Network (ULACNet)

UNKNOWN

Sponsor Role collaborator

University of California, San Francisco

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Joel Palefsky, MD

Role: PRINCIPAL_INVESTIGATOR

University of California, San Francisco

Locations

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Condesa Specialized Clinic

Mexico City, Mexico City, Mexico

Site Status

Condesa Iztapalapa Specialized Clinic

Mexico City, Mexico City, Mexico

Site Status

Instituto Nacional de Cancerologia (INCan)

Mexico City, Mexico City, Mexico

Site Status

University of Puerto Rico Comprehensive Cancer Center

San Juan, PR, Puerto Rico

Site Status

Countries

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United States Mexico Puerto Rico

Other Identifiers

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NCI-2020-13805

Identifier Type: REGISTRY

Identifier Source: secondary_id

ULACNet-101

Identifier Type: OTHER

Identifier Source: secondary_id

U54CA242646

Identifier Type: NIH

Identifier Source: secondary_id

View Link

21622

Identifier Type: -

Identifier Source: org_study_id