Neutrophil Extracellular Traps Formation in Breast Cancer Patients Taking Tamoxifen

NCT ID: NCT05056857

Last Updated: 2025-12-26

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

290 participants

Study Classification

OBSERVATIONAL

Study Start Date

2021-10-01

Study Completion Date

2026-10-31

Brief Summary

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This study examines the long-term effects of tamoxifen (TAM) treatment on excessive production of neutrophil extracellular traps (NET) and their impact on breast cancer and side effects. NET are produced by the body to fight infections but have also been linked to side effects caused by the body's immune system. Treatment with tamoxifen increases the production of NETs. This study may help researchers determine if the increased number of NETs in the body has a damaging effect in breast cancer.

Detailed Description

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PRIMARY OBJECTIVE:

I. To examine the effect of long-term tamoxifen (TAM) treatment on excessive NET formation in breast cancer patients.

SECONDARY OBJECTIVES:

I. To understand the molecular mechanisms of tamoxifen-induced NET formation in breast cancer patients by examining the effect of long-term TAM treatment on the NET-induced factors.

II. To correlate the extend of NET formation with clinical data on tamoxifen resistance, drug side-effects, cancer metastasis and comorbidities.

EXPLORATORY OBJECTIVE:

I. To explore the association between the extent of NET formation and clinical data for breast cancer patients treated with TAM in combination with other drugs.

OUTLINE:

Patients undergo collection of blood samples and their medical charts are reviewed.

Conditions

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Breast Carcinoma

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

OTHER

Study Groups

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Observational (biospecimen collection, medical chart review)

Patients undergo collection of blood samples and their medical charts are reviewed.

Biospecimen Collection

Intervention Type PROCEDURE

Undergo collection of blood sample

Electronic Health Record Review

Intervention Type OTHER

Medical charts are reviewed

Interventions

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Biospecimen Collection

Undergo collection of blood sample

Intervention Type PROCEDURE

Electronic Health Record Review

Medical charts are reviewed

Intervention Type OTHER

Other Intervention Names

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Biological Sample Collection

Eligibility Criteria

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Inclusion Criteria

* Female
* Age criteria for pre-menopausal group: Equal to or greater than 18 years of age and less than or equal to 45 years of age. Patients of age 46-50 will be included if they have not had menstrual cessation for 12 consecutive months.
* Age criteria for menopausal group: At least 51 years of age (median age of menopause). Menopause is defined as cessation of menstrual cycle for 12 consecutive months.
* Diagnosed with ER+ breast cancer
* Being treated with tamoxifen (TAM) for at least 6 months
* CONTROL SUBJECTS: Newly diagnosed ER+ breast cancer patients of the same age group as above on TAM for 0-6 months. This criterion is based on our preliminary results showing that patients taking TAM for 6-7 months exhibit near baseline level of NETs

Exclusion Criteria

* Pregnant -The immune modulations geared toward maintenance of pregnancy are known to cause wide-spread alterations in innate and adaptive immune cell functions. In this scenario, divorcing the pregnancy-related changes in myeloid cell function from those relevant to sepsis and cancer will be complicated.
* History of severe congenital neutropenia due to genetic disorders, such as Kostmann Disorder (HAX1 gene mutation), ELA2 gene mutation, Wiskott-Aldrich syndrome (WAS), Growth Factor Independent 1 Protein (GFI1) gene mutation, Colony Stimulating Factor 3 Receptor (CSF3R) gene mutation, Schwachman-Diamond Syndrome, Barth Syndrome, WHIM Syndrome, and Chadiak-Higashi Syndrome (this list notably does not include Myelodysplastic Syndrome, or Acute/Chronic Myeloid Leukemia)
* History of autoimmune disorders, which can affect the body's inflammatory response, such as rheumatoid arthritis, lupus, Crohn's disease, multiple sclerosis, and psoriasis.
* History of chronic viral infections (human immunodeficiency virus \[HIV\], hepatitis), which can lead to reduced or variable immune cell function.
* A recent positive coronavirus disease (COVID) test
Minimum Eligible Age

18 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

Yes

Sponsors

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National Cancer Institute (NCI)

NIH

Sponsor Role collaborator

National Institute of Allergy and Infectious Diseases (NIAID)

NIH

Sponsor Role collaborator

M.D. Anderson Cancer Center

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Jyotika Sharma

Role: PRINCIPAL_INVESTIGATOR

M.D. Anderson Cancer Center

Locations

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M D Anderson Cancer Center

Houston, Texas, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Jyotika Sharma

Role: CONTACT

Phone: 281-787-7774

Email: [email protected]

Facility Contacts

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Jyotika Sharma

Role: primary

Related Links

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http://www.mdanderson.org

M D Anderson Cancer Center

Other Identifiers

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2021-0491

Identifier Type: -

Identifier Source: org_study_id

NCI-2021-09117

Identifier Type: REGISTRY

Identifier Source: secondary_id

2021-0491

Identifier Type: OTHER

Identifier Source: secondary_id