Assessing the Potential Role of the Gut Microbiome in Modulating Physical Abilities in Humans

NCT ID: NCT05024188

Last Updated: 2021-12-28

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

50 participants

Study Classification

INTERVENTIONAL

Study Start Date

2022-01-20

Study Completion Date

2023-01-20

Brief Summary

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The intestinal microbiome is a microbial system that is influenced by host genetics and environmental exposures such as nutrition, stress and medications. There is a growing body of evidence indicating the significant contribution of the gut microbiome to host health and disease. Furthermore, it has been shown that exercise may modify the microbiome composition. However, important mechanistic questions related to the possible associations between exercise and the human gut microbiome remain unanswered.

In this study, the investigators are using advanced state-of-the-art measurements of physical activity level and related metabolic parameters whether there is a connection between the microbiome and physical abilities in healthy participants and whether antibiotics consumption can influence host physical abilities and glycemic responses through changes induced in microbiome composition and function.

Detailed Description

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During all days of the study, participants will be connected to a continuous glucose monitor (CGM).

In the second segment of the study, participants will be randomized into two groups: antibiotics or placebo, which will be consumed for 7 days.

The third segment of the trial, participants will undergo an FMT/placebo administration for three consecutive days according to their randomization: participants who received antibiotics will receive FMT and participants who received placebo pills will receive placebo capsules in this segment as well.

During the study, participants will collect stool and buccal samples which will be used for microbiota profiling.

Furthermore, participants will undergo physical abilities test, anthropometric measurements, medical history questioners, blood and urine samples and Dual X-ray absorptiometry (DXA) test.

Conditions

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Physical Abilities FMT

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

DOUBLE

Participants Investigators

Study Groups

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Antibiotics + FMT

7 days of antibiotics- Ciprofloxacin, 500 mg 2/day \& Metronidazole (Flagyl), 500 mg 3/day. After antibiotics administration, participants will receive 10 aFMT capsules for three consecutive days (a total of 30 capsules).

Group Type EXPERIMENTAL

Antibiotics

Intervention Type DRUG

Ciprofloxacin, 500 mg 2/day \& Metronidazole (Flagyl), 500 mg 3/day.

Fecal Microbiota Transplantation

Intervention Type OTHER

Fecal Microbiota Transplantation is the process of transferring stool from a healthy donor to another.

Placebo

7 days of cellules pills. After cellules pills administration, participants will receive 10 agarose capsules for three consecutive days (a total of 30 capsules).

Group Type PLACEBO_COMPARATOR

Cellules Pills

Intervention Type OTHER

7 days of cellules placebo pills.

Agarose Capsules

Intervention Type OTHER

Placebo capsules consist a combination of agarose in normal saline/glycerol (the same vehicle as in a FMT capsules)

Interventions

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Antibiotics

Ciprofloxacin, 500 mg 2/day \& Metronidazole (Flagyl), 500 mg 3/day.

Intervention Type DRUG

Fecal Microbiota Transplantation

Fecal Microbiota Transplantation is the process of transferring stool from a healthy donor to another.

Intervention Type OTHER

Cellules Pills

7 days of cellules placebo pills.

Intervention Type OTHER

Agarose Capsules

Placebo capsules consist a combination of agarose in normal saline/glycerol (the same vehicle as in a FMT capsules)

Intervention Type OTHER

Other Intervention Names

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Ciprofloxacin & Metronidazole FMT

Eligibility Criteria

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Inclusion Criteria

* BMI\<28
* Age - 20-35
* Are used to exercise in aerobic sports (running, swimming, Zumba, ball games, functional training, surfing, tennis, wrestling ext.).
* Exercise at least twice a week, for a minimum duration of the last 6 months.

Exclusion Criteria

* Consumption of antibiotics (PO) or probiotics 3 months prior to the first day of the experiment.
* Diagnosis of type 1 or type 2 diabetes.
* Pregnancy, fertility treatments, breastfeeding women six months prior to enrollment and during the study.
* Chronic disease (e.g. AIDS, Cushing syndrome, CKD, acromegaly, hyperthyroidism etc.)
* Cancer and recent anticancer treatment
* Psychiatric disorders
* Coagulation disorders
* Gastrointestinal disorders
* Bariatric surgery
* Alcohol or substance abuse
* BMI\>28
* Any physical condition precluding the completion of a routine effort test
Minimum Eligible Age

20 Years

Maximum Eligible Age

35 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Weizmann Institute of Science

OTHER

Sponsor Role collaborator

Assaf Harofeh MC

OTHER_GOV

Sponsor Role lead

Responsible Party

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Assaf Harofeh MC

Principal Investigator

Responsibility Role SPONSOR_INVESTIGATOR

Principal Investigators

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Ilan Youngster, Dr.

Role: PRINCIPAL_INVESTIGATOR

Assaf-Harofeh MC, Bee'r Yaakov Israel

Central Contacts

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Ilan Youngster, Dr.

Role: CONTACT

Phone: +972-50630119

Email: [email protected]

References

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Clarke SF, Murphy EF, O'Sullivan O, Lucey AJ, Humphreys M, Hogan A, Hayes P, O'Reilly M, Jeffery IB, Wood-Martin R, Kerins DM, Quigley E, Ross RP, O'Toole PW, Molloy MG, Falvey E, Shanahan F, Cotter PD. Exercise and associated dietary extremes impact on gut microbial diversity. Gut. 2014 Dec;63(12):1913-20. doi: 10.1136/gutjnl-2013-306541. Epub 2014 Jun 9.

Reference Type BACKGROUND
PMID: 25021423 (View on PubMed)

Barton W, Penney NC, Cronin O, Garcia-Perez I, Molloy MG, Holmes E, Shanahan F, Cotter PD, O'Sullivan O. The microbiome of professional athletes differs from that of more sedentary subjects in composition and particularly at the functional metabolic level. Gut. 2018 Apr;67(4):625-633. doi: 10.1136/gutjnl-2016-313627. Epub 2017 Mar 30.

Reference Type BACKGROUND
PMID: 28360096 (View on PubMed)

Other Identifiers

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0277-19-ASF

Identifier Type: -

Identifier Source: org_study_id

NCT04707222

Identifier Type: -

Identifier Source: nct_alias