Association of Adjuvant Radiotherapy of Non-metastatic Breast Carcinoma With Immunomodulation and Circulating Tumor Cell Phenotype in Relation to Patient Age.

NCT ID: NCT04902937

Last Updated: 2023-11-28

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

200 participants

Study Classification

OBSERVATIONAL

Study Start Date

2021-07-01

Study Completion Date

2026-12-31

Brief Summary

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In a prospective study, the influence of adjuvant radiotherapy in patients with non-metastatic breast carcinoma on the epithelial tumor cells (CETCs) circulating in the blood and their immunohistochemical characteristics depending on age will be investigated. In addition to a histopathological assignment of the CETCs as cells of the primary tumor, major trigger points of the immune system will be exploratively analyzed.

For this purpose, blood samples are taken from the patients at different time points after tumor resection and during adjuvant radiotherapy. In addition to the detection, isolation and genetic characterization of the CETCs, the determination of immunological biomarkers by immunophenotyping, among other methods, is planned. Furthermore, analyses of tissue from the primary tumor with respect to immunohistochemical features as well as tumor-infiltrating lymphocytes (TILs) are planned. The results will be classified and correlated especially with regard to patient age.

As there are insufficient data available for breast carcinoma regarding radiotherapeutic effects on the immune system depending on patient age, it is of great interest to better understand these molecular biological basics in order to identify potential prognostic biomarkers.

Detailed Description

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The primary aim of this prospective-explorative phase 0 study is to investigate the influence of adjuvant radiotherapy in patients with non-metastatic breast cancer on the circulating epithelial tumor cells (CETCs) in the peripheral blood and their immunohistochemical characteristics in younger high risk (premenopausal) versus older low risk (postmenopausal) patients. In addition, the immunophenotype will be determined using peripheral venous blood mononuclear cells (PBMCs) at different time points during the course of therapy and correlated with the results of CETC analysis. The aim of the study is to identify prognostic and predictive biomarkers for early stage breast cancer patients. No therapeutic consequences will be derived.

For this purpose, the phenotype and molecular biological profile of CETC will be investigated and compared with the corresponding characteristics of the primary tumor in order to assign the tumor cells detected in the peripheral blood to the primary tumor. In this context, the number of tumor-infiltrating lymphocytes (TILs) will be correlated with circulating tumor cells and immunophenotype.

Another goal is the characterization of individually isolated CETC by RT-PCR. The expression of selected marker genes involved in metastatic processes will be investigated during the course of therapy and compared with that of the primary tumor to identify therapy-associated changes. The in vitro cultivation of so-called tumor spheres and their characterization as a sign of a therapy-resistant phenotype is a secondary objective of the study.

Conditions

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Tumor Cell, Circulating

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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premenopausal patients

Adjuvant radiotherapy

Intervention Type RADIATION

Guideline-based therapy of primary non-metastatic early breast carcinoma

neoadjuvant and adjuvant chemotherapy

Intervention Type DRUG

Guideline-based therapy of primary non-metastatic early breast carcinoma

postmenopausal patients

Adjuvant radiotherapy

Intervention Type RADIATION

Guideline-based therapy of primary non-metastatic early breast carcinoma

neoadjuvant and adjuvant chemotherapy

Intervention Type DRUG

Guideline-based therapy of primary non-metastatic early breast carcinoma

Interventions

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Adjuvant radiotherapy

Guideline-based therapy of primary non-metastatic early breast carcinoma

Intervention Type RADIATION

neoadjuvant and adjuvant chemotherapy

Guideline-based therapy of primary non-metastatic early breast carcinoma

Intervention Type DRUG

Other Intervention Names

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breast-conserving therapy

Eligibility Criteria

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Inclusion Criteria

* Primary diagnosis histologically confirmed invasive breast carcinoma pT1-4 pN0 or pN+ cM0
* curative-intended breast-conserving therapy (BET) ± (neo-) adj. system therapy with subsequent adjuvant radiotherapy of the breast ± lymphatic drainage system planned
* informed consent of the patient

Exclusion Criteria

* DCIS, Inflammatory breast carcinoma
* Recurrence situation
* Presence of distant metastases
* Second malignancies \< 10 years prior to diagnosis of breast carcinoma
* Prior exposure to radiotherapy
Minimum Eligible Age

18 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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University Hospital Erlangen

OTHER

Sponsor Role collaborator

Labor Prof. Pachmann

UNKNOWN

Sponsor Role collaborator

University of Jena

OTHER

Sponsor Role lead

Responsible Party

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Matthias Mäurer

Dr. med.

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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Department of Radiooncology, Jena

Jena, , Germany

Site Status RECRUITING

Countries

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Germany

Facility Contacts

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Matthias A Mäurer, MD

Role: primary

+4936419328401

Other Identifiers

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CETC 002

Identifier Type: -

Identifier Source: org_study_id