Trial Outcomes & Findings for Study to Evaluate the Effects of RO7496998 (AT-527) in Non-Hospitalized Adult and Adolescent Participants With Mild or Moderate COVID-19 (NCT NCT04889040)

NCT ID: NCT04889040

Last Updated: 2024-01-05

Results Overview

COVID-19 symptoms will be evaluated using the COVID-19 Symptom Diary (questions 1-12). The severity of each COVID-19 symptom will be recorded on a Likert scale (i.e none/mild/moderate/severe). The time to alleviation or improvement of COVID-19 symptoms is defined as follows: for new symptoms, it is defined as the length of time taken from randomization to the point at which a Score of 0 or 1 has been maintained for a duration of at least 21.5 hours. For preexisting symptoms, it is defined as the time from randomization to when a patient's symptoms have been maintained or improved (requires at least a single category improvement from baseline on the COVID-19 Symptom Diary Likert scale) for a duration of 21.5 hours.

Recruitment status

TERMINATED

Study phase

PHASE3

Target enrollment

216 participants

Primary outcome timeframe

Up to 29 days

Results posted on

2024-01-05

Participant Flow

Participants were non-hospitalized, with mild to moderate COVID-19, with or without risk factors for severe disease.

Participant milestones

Participant milestones
Measure
Placebo
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
Orally administered, 550 mg twice daily (BID) for 5 days
Overall Study
STARTED
73
143
Overall Study
Received Treatment
71
141
Overall Study
COMPLETED
68
138
Overall Study
NOT COMPLETED
5
5

Reasons for withdrawal

Reasons for withdrawal
Measure
Placebo
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
Orally administered, 550 mg twice daily (BID) for 5 days
Overall Study
Withdrawal by Subject
3
3
Overall Study
Lost to Follow-up
0
1
Overall Study
Adverse Event
2
1

Baseline Characteristics

All participants who were randomized to treatment, received a dose of study treatment, were reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) positive for SARS-CoV-2 at any point during the study and did not have missing data.

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Placebo
n=73 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
n=143 Participants
Orally administered, 550 mg twice daily (BID) for 5 days
Total
n=216 Participants
Total of all reporting groups
Age, Continuous
41.7 years
STANDARD_DEVIATION 14.9 • n=73 Participants
41.1 years
STANDARD_DEVIATION 13.2 • n=143 Participants
41.3 years
STANDARD_DEVIATION 13.7 • n=216 Participants
Sex: Female, Male
Female
32 Participants
n=73 Participants
62 Participants
n=143 Participants
94 Participants
n=216 Participants
Sex: Female, Male
Male
41 Participants
n=73 Participants
81 Participants
n=143 Participants
122 Participants
n=216 Participants
Race (NIH/OMB)
American Indian or Alaska Native
6 Participants
n=73 Participants
9 Participants
n=143 Participants
15 Participants
n=216 Participants
Race (NIH/OMB)
Asian
14 Participants
n=73 Participants
26 Participants
n=143 Participants
40 Participants
n=216 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
1 Participants
n=73 Participants
0 Participants
n=143 Participants
1 Participants
n=216 Participants
Race (NIH/OMB)
Black or African American
1 Participants
n=73 Participants
3 Participants
n=143 Participants
4 Participants
n=216 Participants
Race (NIH/OMB)
White
50 Participants
n=73 Participants
105 Participants
n=143 Participants
155 Participants
n=216 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=73 Participants
0 Participants
n=143 Participants
0 Participants
n=216 Participants
Race (NIH/OMB)
Unknown or Not Reported
1 Participants
n=73 Participants
0 Participants
n=143 Participants
1 Participants
n=216 Participants
Number of COVID-19 Symptoms Present at Baseline
7.85 Number of Covid-19 Symptoms
STANDARD_DEVIATION 2.58 • n=68 Participants • All participants who were randomized to treatment, received a dose of study treatment, were reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) positive for SARS-CoV-2 at any point during the study and did not have missing data.
7.92 Number of Covid-19 Symptoms
STANDARD_DEVIATION 2.74 • n=130 Participants • All participants who were randomized to treatment, received a dose of study treatment, were reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) positive for SARS-CoV-2 at any point during the study and did not have missing data.
7.90 Number of Covid-19 Symptoms
STANDARD_DEVIATION 2.68 • n=198 Participants • All participants who were randomized to treatment, received a dose of study treatment, were reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) positive for SARS-CoV-2 at any point during the study and did not have missing data.

PRIMARY outcome

Timeframe: Up to 29 days

Population: All participants who were randomized to treatment, received a dose of study treatment, and were RT-qPCR positive for SARS-CoV-2 at any point during the study.

COVID-19 symptoms will be evaluated using the COVID-19 Symptom Diary (questions 1-12). The severity of each COVID-19 symptom will be recorded on a Likert scale (i.e none/mild/moderate/severe). The time to alleviation or improvement of COVID-19 symptoms is defined as follows: for new symptoms, it is defined as the length of time taken from randomization to the point at which a Score of 0 or 1 has been maintained for a duration of at least 21.5 hours. For preexisting symptoms, it is defined as the time from randomization to when a patient's symptoms have been maintained or improved (requires at least a single category improvement from baseline on the COVID-19 Symptom Diary Likert scale) for a duration of 21.5 hours.

Outcome measures

Outcome measures
Measure
Placebo
n=70 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
n=137 Participants
Orally administered, 550 mg twice daily (BID) for 5 days
Time to Alleviation or Improvement of COVID-19 Symptoms (21.5 Hours)
73.7 hours
Interval 47.1 to 105.8
94.5 hours
Interval 68.1 to 131.7

SECONDARY outcome

Timeframe: Up to 29 days

Population: All participants who were randomized to treatment, received a dose of study treatment, and were RT-qPCR positive for SARS-CoV-2 at any point during the study.

COVID-19 symptoms will be evaluated using the COVID-19 Symptom Diary (questions 1-12). The severity of each COVID-19 symptom will be recorded on a Likert scale (i.e none/mild/moderate/severe). The time to alleviation or improvement of COVID-19 symptoms is defined as follows: for new symptoms, it is defined as the length of time taken from randomization to the point at which a Score of 0 or 1 has been maintained for a duration of at least 43 hours. For preexisting symptoms, it is defined as the time from randomization to when a patient's symptoms have been maintained or improved (requires at least a single category improvement from baseline on the COVID-19 Symptom Diary Likert scale) for a duration of 43 hours.

Outcome measures

Outcome measures
Measure
Placebo
n=70 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
n=137 Participants
Orally administered, 550 mg twice daily (BID) for 5 days
Time to Alleviation or Improvement of COVID-19 Symptoms (43 Hours)
79.6 hours
Interval 55.7 to 108.2
109.5 hours
Interval 83.8 to 147.5

SECONDARY outcome

Timeframe: Up to 29 days

Population: All participants who were randomized to treatment, received a dose of study treatment, and were RT-qPCR positive for SARS-CoV-2 at any point during the study.

Time from randomization to the point at which the following criterion is met and maintained for at least 21.5 hours. \- Score of 0 or 1 on Items 1-12 of the COVID-19 Symptom Diary.

Outcome measures

Outcome measures
Measure
Placebo
n=70 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
n=137 Participants
Orally administered, 550 mg twice daily (BID) for 5 days
Time to Alleviation of COVID-19 Symptoms (21.5 Hours)
73.7 hours
Interval 47.1 to 105.8
94.5 hours
Interval 68.1 to 131.7

SECONDARY outcome

Timeframe: Up to 29 days

Population: All participants who were randomized to treatment, received a dose of study treatment, and were RT-qPCR positive for SARS-CoV-2 at any point during the study.

Time from randomization to the point at which the following criterion is met and maintained for at least 43 hours. \- Score of 0 or 1 on Items 1-12 of the COVID-19 Symptom Diary.

Outcome measures

Outcome measures
Measure
Placebo
n=70 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
n=137 Participants
Orally administered, 550 mg twice daily (BID) for 5 days
Time to Alleviation of COVID-19 Symptoms (43 Hours)
79.6 hours
Interval 55.7 to 108.2
109.5 hours
Interval 83.8 to 147.5

SECONDARY outcome

Timeframe: Up to 29 days

Population: All participants who were randomized to treatment, received a dose of study treatment, were RT-qPCR positive for SARS-CoV-2 at any point during the study and with baseline symptoms.

Time from randomization to the point at which symptoms (Items 1-12 of the COVID-19 Symptom Diary) have improved by at least one category from baseline on the COVID-19 Symptom Diary Likert scale, maintained for at least 21.5 hours.

Outcome measures

Outcome measures
Measure
Placebo
n=68 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
n=130 Participants
Orally administered, 550 mg twice daily (BID) for 5 days
Time to One-Category Improvement of Baseline Presenting COVID-19 Symptoms
70.9 hours
Interval 41.8 to 91.8
81.5 hours
Interval 60.5 to 119.9

SECONDARY outcome

Timeframe: Up to 29 days

Population: All participants who were randomized to treatment, received a dose of study treatment, were RT-qPCR positive for SARS-CoV-2 at any point during the study and had a symptom score of \>= 2 at baseline.

Time from randomization to the point at which the following criterion is met and maintained (for each individual symptom) for at least 21.5 hours. \- Score of 0 or 1 for Items 1-14 of the COVID-19 Symptom Diary

Outcome measures

Outcome measures
Measure
Placebo
n=70 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
n=137 Participants
Orally administered, 550 mg twice daily (BID) for 5 days
Time to Alleviation of Individual Symptoms
Nasal Congestion or Runny Nose
36.4 hours
Interval 23.4 to 60.6
37.3 hours
Interval 23.4 to 57.6
Time to Alleviation of Individual Symptoms
Sore Throat
45.6 hours
Interval 23.4 to 75.1
33.2 hours
Interval 20.9 to 54.3
Time to Alleviation of Individual Symptoms
Cough
37.6 hours
Interval 26.8 to 118.7
49.5 hours
Interval 28.4 to 106.4
Time to Alleviation of Individual Symptoms
Shortness of Breath
24.0 hours
Interval 11.8 to 106.8
47.5 hours
Interval 32.4 to 96.8
Time to Alleviation of Individual Symptoms
Muscle or Body Aches
35.3 hours
Interval 14.3 to 59.4
44.9 hours
Interval 33.1 to 53.0
Time to Alleviation of Individual Symptoms
Fatigue
66.6 hours
Interval 36.5 to 118.9
61.3 hours
Interval 47.1 to 108.1
Time to Alleviation of Individual Symptoms
Headache
23.2 hours
Interval 14.5 to 41.8
41.4 hours
Interval 24.4 to 59.5
Time to Alleviation of Individual Symptoms
Chills/Sweats
36.0 hours
Interval 22.5 to 57.6
39.0 hours
Interval 28.4 to 50.5
Time to Alleviation of Individual Symptoms
Feeling Hot or Feverish
24.6 hours
Interval 16.4 to 66.7
36.2 hours
Interval 19.7 to 61.4
Time to Alleviation of Individual Symptoms
Nausea
31.4 hours
Interval 11.1 to
Not evaluable due to an insufficient number of events
43.5 hours
Interval 22.6 to 73.4
Time to Alleviation of Individual Symptoms
Vomiting
20.1 hours
Interval 16.4 to
Not evaluable due to an insufficient number of events
33.0 hours
Not evaluable due to an insufficient number of events
Time to Alleviation of Individual Symptoms
Diarrhea
17.6 hours
Interval 14.9 to 73.6
66.7 hours
Interval 19.8 to 107.2
Time to Alleviation of Individual Symptoms
Sense of Smell
132.6 hours
Interval 84.3 to 229.0
104.9 hours
Interval 72.6 to 206.0
Time to Alleviation of Individual Symptoms
Sense of Taste
128.9 hours
Interval 84.3 to 202.2
155.7 hours
Interval 52.5 to 371.7

SECONDARY outcome

Timeframe: Up to Day 33 visit

Population: All participants who were randomized to treatment, received a dose of study treatment, and were RT-qPCR positive for SARS-CoV-2 at any point during the study.

Hospitalizations for COVID-19 are defined as SAEs for which the investigator has cited that the suspected cause was the disease under study and where there is a non-missing hospital admission date.

Outcome measures

Outcome measures
Measure
Placebo
n=70 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
n=137 Participants
Orally administered, 550 mg twice daily (BID) for 5 days
Percentage of Participants Requiring Hospitalization for COVID-19
10.0 percentage of participants
Interval 1.25 to 18.75
2.9 percentage of participants
Interval 0.0 to 6.51

SECONDARY outcome

Timeframe: Up to Day 33 visit

Population: All participants who were randomized to treatment, received a dose of study treatment, and were RT-qPCR positive for SARS-CoV-2 at any point during the study.

Medically attended visit is defined as hospitalization, emergency room (ER) visit, urgent care visit, physician's office visit, or telemedicine visit, with the primary reason for the visit being COVID-19.

Outcome measures

Outcome measures
Measure
Placebo
n=70 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
n=137 Participants
Orally administered, 550 mg twice daily (BID) for 5 days
Percentage of Participants With Greater Than or Equal to 1 COVID-19 Related Medically Attended Visit
14.3 percentage of participants
Interval 4.2 to 24.37
10.2 percentage of participants
Interval 4.05 to 16.38

SECONDARY outcome

Timeframe: Up to 29 days

Population: All participants who were randomized to treatment, received a dose of study treatment, were RT-qPCR positive for SARS-CoV-2 at any point during the study, and had a fever at baseline.

Time to return to an afebrile state (temperature ≤ 37.5°C) maintained for at least 21.5 hours.

Outcome measures

Outcome measures
Measure
Placebo
n=12 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
n=20 Participants
Orally administered, 550 mg twice daily (BID) for 5 days
Duration of Fever
47.3 hours
Interval 12.6 to 82.2
55.7 hours
Interval 25.9 to 96.4

SECONDARY outcome

Timeframe: Up to Day 33 visit

Population: All participants who were randomized to treatment, received a dose of study treatment, and were RT-qPCR positive for SARS-CoV-2 at any point during the study.

COVID-related complications are defined as death, hospitalization, pneumonia, sepsis, coagulopathy, pericarditis/myocarditis and cardiac failure. Pneumonia, acute respiratory failure, sepsis, coagulopathy, pericarditis/myocarditis, and cardiac failure were adjudicated per blinded manual medical review of events by an internal adjudication team before study readout.

Outcome measures

Outcome measures
Measure
Placebo
n=70 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
n=137 Participants
Orally administered, 550 mg twice daily (BID) for 5 days
Percentage of Participants With COVID-19 Related Complications
10.0 percentage of participants
Interval 1.25 to 18.75
4.4 percentage of participants
Interval 0.1 to 8.66

SECONDARY outcome

Timeframe: Up to Day 33 visit

Population: All participants who were randomized to treatment, received a dose of study treatment, and were RT-qPCR positive for SARS-CoV-2 at any point during the study.

Post-treatment infections were defined as any treatment-emergent adverse event with a primary system organ class of infections and infestations.

Outcome measures

Outcome measures
Measure
Placebo
n=70 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
n=137 Participants
Orally administered, 550 mg twice daily (BID) for 5 days
Percentage of Participants With Any Post-Treatment Infection
14.3 percentage of participants
Interval 4.2 to 24.37
9.5 percentage of participants
Interval 3.51 to 15.47

SECONDARY outcome

Timeframe: Baseline and on Days 3, 5, 7 and 14

Population: All participants who were randomized to treatment, received a dose of study treatment, and were RT-qPCR positive for SARS-CoV-2 at any point during the study.

SARS-CoV-2 virus RNA will be measured by reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR)

Outcome measures

Outcome measures
Measure
Placebo
n=70 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
n=137 Participants
Orally administered, 550 mg twice daily (BID) for 5 days
Change From Baseline in the Amount of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) Virus RNA
Baseline
6.49 log10 copies/mL
Standard Deviation 1.61
6.59 log10 copies/mL
Standard Deviation 1.54
Change From Baseline in the Amount of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) Virus RNA
Day 3
-1.05 log10 copies/mL
Standard Deviation 1.06
-1.07 log10 copies/mL
Standard Deviation 1.16
Change From Baseline in the Amount of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) Virus RNA
Day 5
-2.24 log10 copies/mL
Standard Deviation 1.40
-2.01 log10 copies/mL
Standard Deviation 1.50
Change From Baseline in the Amount of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) Virus RNA
Day 7
-3.10 log10 copies/mL
Standard Deviation 1.38
-2.98 log10 copies/mL
Standard Deviation 1.66
Change From Baseline in the Amount of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) Virus RNA
Day 14
-4.21 log10 copies/mL
Standard Deviation 1.64
-4.25 log10 copies/mL
Standard Deviation 1.64

SECONDARY outcome

Timeframe: Up to 14 days

Population: All participants who were randomized to treatment, received a dose of study treatment, and were RT-qPCR positive for SARS-CoV-2 at any point during the study.

Defined as time from randomization to the first time when a negative qualitative virus RNA by RT-PCR test result is obtained.

Outcome measures

Outcome measures
Measure
Placebo
n=70 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
n=137 Participants
Orally administered, 550 mg twice daily (BID) for 5 days
Time to Cessation of SARS-CoV-2 Viral Shedding
13.0 days
Interval 12.8 to
Not evaluable due to an insufficient number of events
13.0 days
Interval 13.0 to 13.1

SECONDARY outcome

Timeframe: Baseline and on Days 3, 5, 7 and 14

Population: All participants who were randomized to treatment, received a dose of study treatment, and were RT-qPCR positive for SARS-CoV-2 at any point during the study.

Defined as percentage of participants with a positive qualitative virus RNA by RT-PCR.

Outcome measures

Outcome measures
Measure
Placebo
n=70 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
n=137 Participants
Orally administered, 550 mg twice daily (BID) for 5 days
Percentage of Participants Positive for SARS-CoV-2 Virus RNA at Specified Timepoints
Baseline
97.1 percentage of participants
100 percentage of participants
Percentage of Participants Positive for SARS-CoV-2 Virus RNA at Specified Timepoints
Day 3
98.5 percentage of participants
96.4 percentage of participants
Percentage of Participants Positive for SARS-CoV-2 Virus RNA at Specified Timepoints
Day 5
89.7 percentage of participants
91.9 percentage of participants
Percentage of Participants Positive for SARS-CoV-2 Virus RNA at Specified Timepoints
Day 7
74.6 percentage of participants
76.3 percentage of participants
Percentage of Participants Positive for SARS-CoV-2 Virus RNA at Specified Timepoints
Day 14
43.9 percentage of participants
34.4 percentage of participants

SECONDARY outcome

Timeframe: Day 1 prior to dosing, Day 3, Day 5, Day 7 and Day 14

Population: All participants who were randomized to treatment, received a dose of study treatment, were RT-qPCR positive for SARS-CoV-2 at any point during the study, and had at least 2 or more virology assessments.

Outcome measures

Outcome measures
Measure
Placebo
n=69 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
n=137 Participants
Orally administered, 550 mg twice daily (BID) for 5 days
Area Under the Curve (AUC) in the Amount of SARS-CoV-2 Virus RNA
1122.39 log10 copies/mL*hour
Standard Deviation 339.70
1174.30 log10 copies/mL*hour
Standard Deviation 377.49

SECONDARY outcome

Timeframe: Up to Day 33 visit

Population: All participants who received at least one dose of study treatment

Outcome measures

Outcome measures
Measure
Placebo
n=71 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
n=141 Participants
Orally administered, 550 mg twice daily (BID) for 5 days
Percentage of Participants With Adverse Events (AEs)
36.6 percentage of participants
39.0 percentage of participants

SECONDARY outcome

Timeframe: Up to 7 days

Population: All participants who received AT-527 and had at least one post-dose drug concentration measurement at a scheduled visit time point

AT-511 is the free base form of RO7496998 (AT-527).

Outcome measures

Outcome measures
Measure
Placebo
n=137 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
Orally administered, 550 mg twice daily (BID) for 5 days
Plasma Concentration of AT-511 at Specified Timepoints
Day 5, 48 hours
4.6 nanograms per milliliter (ng/mL)
Standard Deviation 40.9
Plasma Concentration of AT-511 at Specified Timepoints
Day 1, 0 hour
17.4 nanograms per milliliter (ng/mL)
Standard Deviation 158.6
Plasma Concentration of AT-511 at Specified Timepoints
Day 1, 1 hour
1456.8 nanograms per milliliter (ng/mL)
Standard Deviation 1757.7
Plasma Concentration of AT-511 at Specified Timepoints
Day 1, 2 hours
1029.3 nanograms per milliliter (ng/mL)
Standard Deviation 1085.5
Plasma Concentration of AT-511 at Specified Timepoints
Day 1, 4 hours
206.3 nanograms per milliliter (ng/mL)
Standard Deviation 289.5
Plasma Concentration of AT-511 at Specified Timepoints
Day 5, 0 hour
52.8 nanograms per milliliter (ng/mL)
Standard Deviation 271.0
Plasma Concentration of AT-511 at Specified Timepoints
Day 5, 1 hour
1659.0 nanograms per milliliter (ng/mL)
Standard Deviation 2213.9
Plasma Concentration of AT-511 at Specified Timepoints
Day 5, 2 hours
1934.5 nanograms per milliliter (ng/mL)
Standard Deviation 2210.6
Plasma Concentration of AT-511 at Specified Timepoints
Day 5, 4 hours
202.3 nanograms per milliliter (ng/mL)
Standard Deviation 310.7
Plasma Concentration of AT-511 at Specified Timepoints
Day 5, 8 hours
8.8 nanograms per milliliter (ng/mL)
Standard Deviation 9.5

SECONDARY outcome

Timeframe: Up to 7 days

Population: All participants who received AT-527 and had at least one post-dose drug concentration measurement at a scheduled visit time point

AT-511 is the free base form of RO7496998 (AT-527). Major metabolites are AT-551, AT-229, and AT-273 (a surrogate for the intracellular concentration of the active triphosphate metabolite AT-9010)

Outcome measures

Outcome measures
Measure
Placebo
n=137 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
Orally administered, 550 mg twice daily (BID) for 5 days
Plasma Concentration of AT-551 at Specified Timepoints
Day 1, 0 hour
1.9 nanograms per milliliter (ng/mL)
Standard Deviation 12.2
Plasma Concentration of AT-551 at Specified Timepoints
Day 1, 1 hour
166.9 nanograms per milliliter (ng/mL)
Standard Deviation 209.8
Plasma Concentration of AT-551 at Specified Timepoints
Day 1, 2 hours
335.7 nanograms per milliliter (ng/mL)
Standard Deviation 257.6
Plasma Concentration of AT-551 at Specified Timepoints
Day 1, 4 hours
195.0 nanograms per milliliter (ng/mL)
Standard Deviation 146.4
Plasma Concentration of AT-551 at Specified Timepoints
Day 5, 0 hour
49.1 nanograms per milliliter (ng/mL)
Standard Deviation 65.0
Plasma Concentration of AT-551 at Specified Timepoints
Day 5, 1 hour
150.2 nanograms per milliliter (ng/mL)
Standard Deviation 144.5
Plasma Concentration of AT-551 at Specified Timepoints
Day 5, 2 hours
213.6 nanograms per milliliter (ng/mL)
Standard Deviation 201.7
Plasma Concentration of AT-551 at Specified Timepoints
Day 5, 4 hours
138.8 nanograms per milliliter (ng/mL)
Standard Deviation 136.2
Plasma Concentration of AT-551 at Specified Timepoints
Day 5, 8 hours
27.5 nanograms per milliliter (ng/mL)
Standard Deviation 17.5
Plasma Concentration of AT-551 at Specified Timepoints
Day 5, 48 hours
2.6 nanograms per milliliter (ng/mL)
Standard Deviation 5.2

SECONDARY outcome

Timeframe: Up to 7 days

Population: All participants who received AT-527 and had at least one post-dose drug concentration measurement at a scheduled visit time point

AT-511 is the free base form of RO7496998 (AT-527). Major metabolites are AT-551, AT-229, and AT-273 (a surrogate for the intracellular concentration of the active triphosphate metabolite AT-9010)

Outcome measures

Outcome measures
Measure
Placebo
n=137 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
Orally administered, 550 mg twice daily (BID) for 5 days
Plasma Concentration of AT-229 at Specified Timepoints
Day 1, 0 hour
2.2 nanograms per milliliter (ng/mL)
Standard Deviation 18.4
Plasma Concentration of AT-229 at Specified Timepoints
Day 1, 1 hour
123.6 nanograms per milliliter (ng/mL)
Standard Deviation 172.5
Plasma Concentration of AT-229 at Specified Timepoints
Day 1, 2 hours
507.8 nanograms per milliliter (ng/mL)
Standard Deviation 497.5
Plasma Concentration of AT-229 at Specified Timepoints
Day 1, 4 hours
354.4 nanograms per milliliter (ng/mL)
Standard Deviation 259.2
Plasma Concentration of AT-229 at Specified Timepoints
Day 5, 0 hour
367.8 nanograms per milliliter (ng/mL)
Standard Deviation 259.1
Plasma Concentration of AT-229 at Specified Timepoints
Day 5, 1 hour
418.0 nanograms per milliliter (ng/mL)
Standard Deviation 257.6
Plasma Concentration of AT-229 at Specified Timepoints
Day 5, 2 hours
454.3 nanograms per milliliter (ng/mL)
Standard Deviation 331.7
Plasma Concentration of AT-229 at Specified Timepoints
Day 5, 4 hours
490.1 nanograms per milliliter (ng/mL)
Standard Deviation 296.4
Plasma Concentration of AT-229 at Specified Timepoints
Day 5, 8 hours
279.6 nanograms per milliliter (ng/mL)
Standard Deviation 176.0
Plasma Concentration of AT-229 at Specified Timepoints
Day 5, 48 hours
118.7 nanograms per milliliter (ng/mL)
Standard Deviation 127.2

SECONDARY outcome

Timeframe: Up to 7 days

Population: All participants who received AT-527 and had at least one post-dose drug concentration measurement at a scheduled visit time point

AT-511 is the free base form of RO7496998 (AT-527). Major metabolites are AT-551, AT-229, and AT-273 (a surrogate for the intracellular concentration of the active triphosphate metabolite AT-9010)

Outcome measures

Outcome measures
Measure
Placebo
n=137 Participants
The dose and regimen of the placebo will match that of AT-527.
RO7496998 (AT-527)
Orally administered, 550 mg twice daily (BID) for 5 days
Plasma Concentration of AT-273 at Specified Timepoints
Day 1, 0 hour
0.7 nanograms per milliliter (ng/mL)
Standard Deviation 2.5
Plasma Concentration of AT-273 at Specified Timepoints
Day 1, 1 hour
15.5 nanograms per milliliter (ng/mL)
Standard Deviation 26.9
Plasma Concentration of AT-273 at Specified Timepoints
Day 1, 2 hours
135.1 nanograms per milliliter (ng/mL)
Standard Deviation 136.2
Plasma Concentration of AT-273 at Specified Timepoints
Day 1, 4 hours
173.5 nanograms per milliliter (ng/mL)
Standard Deviation 120.6
Plasma Concentration of AT-273 at Specified Timepoints
Day 5, 0 hour
149.8 nanograms per milliliter (ng/mL)
Standard Deviation 78.1
Plasma Concentration of AT-273 at Specified Timepoints
Day 5, 1 hour
141.7 nanograms per milliliter (ng/mL)
Standard Deviation 72.7
Plasma Concentration of AT-273 at Specified Timepoints
Day 5, 2 hours
108.8 nanograms per milliliter (ng/mL)
Standard Deviation 61.0
Plasma Concentration of AT-273 at Specified Timepoints
Day 5, 4 hours
172.7 nanograms per milliliter (ng/mL)
Standard Deviation 129.2
Plasma Concentration of AT-273 at Specified Timepoints
Day 5, 8 hours
118.6 nanograms per milliliter (ng/mL)
Standard Deviation 80.2
Plasma Concentration of AT-273 at Specified Timepoints
Day 5, 48 hours
48.4 nanograms per milliliter (ng/mL)
Standard Deviation 32.7

Adverse Events

Placebo

Serious events: 7 serious events
Other events: 5 other events
Deaths: 0 deaths

AT-527

Serious events: 5 serious events
Other events: 11 other events
Deaths: 0 deaths

Serious adverse events

Serious adverse events
Measure
Placebo
n=71 participants at risk
The dose and regimen of the placebo will match that of AT-527.
AT-527
n=141 participants at risk
Orally administered, 550 mg twice daily (BID) for 5 days
Infections and infestations
COVID-19
4.2%
3/71 • Number of events 3 • up to the Day 33 visit
Safety analyses were performed on the safety evaluable set, which consisted of all randomized participants who received at least one dose of study treatment.
1.4%
2/141 • Number of events 2 • up to the Day 33 visit
Safety analyses were performed on the safety evaluable set, which consisted of all randomized participants who received at least one dose of study treatment.
Infections and infestations
COVID-19 pneumonia
5.6%
4/71 • Number of events 4 • up to the Day 33 visit
Safety analyses were performed on the safety evaluable set, which consisted of all randomized participants who received at least one dose of study treatment.
0.71%
1/141 • Number of events 1 • up to the Day 33 visit
Safety analyses were performed on the safety evaluable set, which consisted of all randomized participants who received at least one dose of study treatment.
Infections and infestations
Pneumonia staphylococcal
0.00%
0/71 • up to the Day 33 visit
Safety analyses were performed on the safety evaluable set, which consisted of all randomized participants who received at least one dose of study treatment.
0.71%
1/141 • Number of events 1 • up to the Day 33 visit
Safety analyses were performed on the safety evaluable set, which consisted of all randomized participants who received at least one dose of study treatment.
Renal and urinary disorders
Acute kidney injury
0.00%
0/71 • up to the Day 33 visit
Safety analyses were performed on the safety evaluable set, which consisted of all randomized participants who received at least one dose of study treatment.
0.71%
1/141 • Number of events 1 • up to the Day 33 visit
Safety analyses were performed on the safety evaluable set, which consisted of all randomized participants who received at least one dose of study treatment.
Respiratory, thoracic and mediastinal disorders
Hypoxia
0.00%
0/71 • up to the Day 33 visit
Safety analyses were performed on the safety evaluable set, which consisted of all randomized participants who received at least one dose of study treatment.
0.71%
1/141 • Number of events 1 • up to the Day 33 visit
Safety analyses were performed on the safety evaluable set, which consisted of all randomized participants who received at least one dose of study treatment.
Respiratory, thoracic and mediastinal disorders
Pneumothorax
0.00%
0/71 • up to the Day 33 visit
Safety analyses were performed on the safety evaluable set, which consisted of all randomized participants who received at least one dose of study treatment.
0.71%
1/141 • Number of events 1 • up to the Day 33 visit
Safety analyses were performed on the safety evaluable set, which consisted of all randomized participants who received at least one dose of study treatment.
Respiratory, thoracic and mediastinal disorders
Respiratory failure
0.00%
0/71 • up to the Day 33 visit
Safety analyses were performed on the safety evaluable set, which consisted of all randomized participants who received at least one dose of study treatment.
0.71%
1/141 • Number of events 1 • up to the Day 33 visit
Safety analyses were performed on the safety evaluable set, which consisted of all randomized participants who received at least one dose of study treatment.

Other adverse events

Other adverse events
Measure
Placebo
n=71 participants at risk
The dose and regimen of the placebo will match that of AT-527.
AT-527
n=141 participants at risk
Orally administered, 550 mg twice daily (BID) for 5 days
Musculoskeletal and connective tissue disorders
Back pain
0.00%
0/71 • up to the Day 33 visit
Safety analyses were performed on the safety evaluable set, which consisted of all randomized participants who received at least one dose of study treatment.
5.7%
8/141 • Number of events 8 • up to the Day 33 visit
Safety analyses were performed on the safety evaluable set, which consisted of all randomized participants who received at least one dose of study treatment.
Nervous system disorders
Headache
7.0%
5/71 • Number of events 5 • up to the Day 33 visit
Safety analyses were performed on the safety evaluable set, which consisted of all randomized participants who received at least one dose of study treatment.
2.8%
4/141 • Number of events 4 • up to the Day 33 visit
Safety analyses were performed on the safety evaluable set, which consisted of all randomized participants who received at least one dose of study treatment.

Additional Information

Medical Communications

Hoffmann-La Roche

Phone: 800 821-8590

Results disclosure agreements

  • Principal investigator is a sponsor employee The Study being conducted under this Agreement is part of the Overall Study. Investigator is free to publish in reputable journals or to present at professional conferences the results of the Study, but only after the first publication or presentation that involves the Overall Study. The Sponsor may request that Confidential Information be deleted and/or the publication be postponed in order to protect the Sponsor's intellectual property rights.
  • Publication restrictions are in place

Restriction type: OTHER