Tissue Collection for Drug Screening and Bioanalysis

NCT ID: NCT04852354

Last Updated: 2025-12-17

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

500 participants

Study Classification

OBSERVATIONAL

Study Start Date

2020-07-22

Study Completion Date

2029-07-22

Brief Summary

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The purpose of this study is to improve upon the knowledge currently available about central nervous system (CNS) tumors. We will study the different characteristics of these tumors using tissue samples collected during surgery and post-autopsy. The aim is to create tumor cell lines and models to test how they respond to different drugs. This research will help improve treatment options and identify new targets for therapy.

Detailed Description

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The goal of this project is to enable the acquisition of tissue available for research with the prospect of enabling the development of new therapeutic avenues for patients diagnosed with cancer in the central nervous system.

During patients' (with a CNS tumor) routine operative procedure, if extra tissue deemed unnecessary for diagnostic or clinical purposes is available, selected samples will undergo DNA and/or RNA extraction and integrity analysis, whole genome and RNA sequencing, DNA and RNA methylome analyses, proteomic analysis, immunoprofiling, and primary culturing of the tumor cells. These cultures may then be used for drug screenings and to create patient-derived xenografts, or for comparisons between tumor and non-tumor patients. Other biological samples may be collected. Tissue collection for drug screening will be extended to post-mortem biospecimens such as whole brain, spinal cord, biofluids (e.g., cerebrospinal fluid and blood) and skin biopsy. These samples will be collected through autopsy donation from pediatric and adult brain tumor patients.

Additionally, saliva samples may be obtained from the parents of pediatric patients with CNS tumors, and biological samples may be collected from pediatric patients without a CNS tumor who are undergoing a neurological procedure. These samples may be used for comparison with samples from patients with CNS tumors.

Conditions

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Central Nervous System Tumor

Study Design

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Observational Model Type

CASE_ONLY

Study Time Perspective

PROSPECTIVE

Study Groups

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Cohort 1: Tumor patients undergoing neurosurgery

Samples of tumor tissue, blood, CSF, saliva, skull, and dura will be taken during neurosurgery from tumor patients meeting the inclusion criteria.

No interventions assigned to this group

Parents of tumor patients

Saliva samples will be taken from parents of tumor patients meeting the inclusion criteria.

No interventions assigned to this group

Cohort 2: Non-Tumor patients undergoing neurosurgery

Blood and CSF samples will be taken from non-tumor patients meeting the inclusion criteria.

No interventions assigned to this group

Cohort 3: Autopsy tumor tissue donation

Post-mortem biospecimens such as whole brain, spinal cord, biofluids (e.g., cerebrospinal fluid and blood) and skin biopsy will be collected through autopsy donation from brain tumor patients meeting the inclusion criteria.

No interventions assigned to this group

Eligibility Criteria

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Inclusion Criteria

1. Subject has presented with a suspected/confirmed neoplastic CNS lesion and is undergoing a neurosurgical procedure in which CNS tissue and/or tumors associated with this tissue will be removed.
2. There must be tissue available in excess of that required by neuropathology for diagnostic purposes.
3. Subject of all ages included.
4. Subjects who are pregnant can be included.
5. Subjects who are neonates (0-4 weeks) can be included.


1. Subject is between 4 weeks and 21 years of age and is undergoing a neurosurgical procedure at WCMC.
2. Subject does not have a CNS tumor diagnosis.
3. Subject should not be pregnant at the time of enrollment.


1. Subject of all ages included
2. Subject has been diagnosed with a neoplastic CNS lesion at the time of passing.
3. Subjects who are pregnant can be included.
4. Subjects who are neonates (0-4 weeks) can be included.


1. Subject is the biological parent of a patient undergoing/ who underwent a neurosurgical procedure in which CNS tissue and/or tumors associated with this tissue will be/ was removed.
2. Subject is the biological parent of patient enrolled in cohort 1.
3. Subject is not pregnant at the time of enrollment.
Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Weill Medical College of Cornell University

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Jeffrey Greenfield, M.D.

Role: PRINCIPAL_INVESTIGATOR

Weill Medical College of Cornell University

Locations

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Weill Cornell Medicine

New York, New York, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Jeffrey Greenfield, M.D.

Role: CONTACT

212-746-2363

Pediatric Neurosurgery

Role: CONTACT

212-746-2363

Facility Contacts

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Jeffrey Greenfield, M.D.

Role: primary

212-746-2363

Pediatric Neurosurgery

Role: backup

212-746-2363

References

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Rajappa P, Cobb WS, Vartanian E, Huang Y, Daly L, Hoffman C, Zhang J, Shen B, Yanowitch R, Garg K, Cisse B, Haddock S, Huse J, Pisapia DJ, Chan TA, Lyden DC, Bromberg JF, Greenfield JP. Malignant Astrocytic Tumor Progression Potentiated by JAK-mediated Recruitment of Myeloid Cells. Clin Cancer Res. 2017 Jun 15;23(12):3109-3119. doi: 10.1158/1078-0432.CCR-16-1508. Epub 2016 Dec 30.

Reference Type BACKGROUND
PMID: 28039266 (View on PubMed)

Other Identifiers

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20-01021289

Identifier Type: -

Identifier Source: org_study_id