Anti-protozoal Effect of Steroid Hormone 20-hydroxyecdysone

NCT ID: NCT04827537

Last Updated: 2025-04-04

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

ACTIVE_NOT_RECRUITING

Clinical Phase

PHASE2/PHASE3

Total Enrollment

200 participants

Study Classification

INTERVENTIONAL

Study Start Date

2021-01-01

Study Completion Date

2027-12-31

Brief Summary

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20-hydroxyecdysone is an ecdysteroid hormone found in invertebrates and plants. 20-hydroxyecdysone exhibits a broad range of biological properties in various in vitro and in vivo models, including anabolic, antioxidant, anti-inflammatory, immunomodulatory, anti-obesity and antidiabetic activities, in addition to acting as neuroprotective and hepatoprotective agent. Ecdysteroids are widely used by athletes as dietary supplements to increase strength and muscle mass during resistance training, to reduce fatigue and to ease recovery. Mhashilkar AS et al. (2016) were revealed anti-parasitic activity of 20-hydroxyecdysone against the human filarial parasites. According to recent searches in academic databases and medical search engines (Google, www.google.com; PubMed, www.ncbi.nlm.nih.gov/pubmed; and Medline, www.medline.com), no studies have been conducted to assess the anti-protozoal activity of 20-hydroxyecdysone.

The aim of the study is to assess anti-protozoal activity of 20-hydroxyecdysone in water sports athletes with giardiasis.

Detailed Description

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The randomized, double-blinded, placebo-controlled clinical study will be conducted by the Uzbek State University of Physical Education and Sport, Institute of the Chemistry of Plant Substances named acad. S. Yu. Yunusov and Republican Specialized Scientific and Practical Medical Center of Epidemiology, Microbiology, Infectious and Parasitic Diseases, Tashkent, Uzbekistan

Both informed and written consents will be obtained from the participants.

Study participants will be included about 200 athletes aged 18 to 27 years with giardiasis. All the participants will be residents of Uzbekistan.

Diagnosis of giardiasis will be based on the results of parasitological examination. Inclusion criteria:presence of giardia intestinalis in the stool samples of participant.

The exclusion criteria: chronic and acute infectious diseases, endocrine diseases, congenital diseases, allergic dermatitis, bronchial asthma, allergic rhinitis and/or conjunctivitis, other skin disorders, history of using vitamin D supplements within three months ago, or use of any medication influencing endocrine parameters.

Measurement of anthropometric indices Demographic data will be obtained in the survey included age, gender, and body mass index (BMI) calculated as weight (kg)/height2 (m2).

Blood Sample Collection and Storage Five milliliters of peripheral venous blood sample will be taken (after 8-12 hours of fasting) from each participant and will be collected into HumaTube Serum Gel - C/A for ELISA. Serum will be appropriately obtained and stored at -20°C maximum of 30 days. All blood samples will be collected before and after therapy.

200 water sports athletes with Giardia infection will be randomized from 1 to 3 groups to receive 20-hydroxyecdysone, metronidazole and placebo preparations, respectively

Conditions

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Giardia Lamblia Infection

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

SINGLE

Participants

Study Groups

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20-hydroxyecdysone group

water sports athletes with Giardia infection will receive 20-hydroxyecdysone (100 mg (one pill) x two times a day orally), 10 consecutive days

Group Type EXPERIMENTAL

20-hydroxyecdysone Oral

Intervention Type DRUG

100 mg (one pill) x two times a day orally, 10 consecutive days

metronidazole group

water sports athletes with Giardia infection will receive metronidazole, 500 mg (one pill) x two times a day orally, 10 consecutive days

, and placebo preparations, respectively

Group Type ACTIVE_COMPARATOR

Metronidazole Oral

Intervention Type DRUG

500 mg (one pill) x two times a day orally, 10 consecutive days

placebo group

water sports athletes with Giardia infection will receive placebo preparation, 100 mg (one pill) x two times a day orally, 10 consecutive days

Group Type PLACEBO_COMPARATOR

Placebo Oral

Intervention Type DRUG

100 mg (one pill) x two times a day orally, 10 consecutive days

Interventions

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20-hydroxyecdysone Oral

100 mg (one pill) x two times a day orally, 10 consecutive days

Intervention Type DRUG

Metronidazole Oral

500 mg (one pill) x two times a day orally, 10 consecutive days

Intervention Type DRUG

Placebo Oral

100 mg (one pill) x two times a day orally, 10 consecutive days

Intervention Type DRUG

Other Intervention Names

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ecdysterone Metronidazole shugar pills

Eligibility Criteria

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Inclusion Criteria

\- a presence of giardia lamblia in stool samples of partisipants

Exclusion Criteria

* chronic and acute infectious diseases,
* endocrine diseases, congenital diseases,
* allergic dermatitis,
* bronchial asthma,
* allergic rhinitis and/or conjunctivitis,
* other skin disorders,
* history of using vitamin D supplements within three months ago, or use of any medication influencing endocrine parameters.
Minimum Eligible Age

18 Years

Maximum Eligible Age

27 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

No

Sponsors

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Research Institute of Epidemiology, Microbiology and Infectious Diseases, Uzbekistan

OTHER_GOV

Sponsor Role lead

Responsible Party

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Svetlana Osipova, MD, PhD, DS

Dr.

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Abdurakhim Toychiev, PhD

Role: STUDY_CHAIR

research institute of epidemiology, microbiology and infectious diseases

Locations

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Research institute of epidemiology, microbiology and infectious diseases

Tashkent, Uchtepa, Uzbekistan

Site Status

Countries

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Uzbekistan

Other Identifiers

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2019121819

Identifier Type: -

Identifier Source: org_study_id

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