CTC Quantification During TURBT and PKVBT of Transitional Cell Carcinoma in Purging Fluid and Blood

NCT ID: NCT04811846

Last Updated: 2024-12-09

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

ACTIVE_NOT_RECRUITING

Clinical Phase

NA

Total Enrollment

40 participants

Study Classification

INTERVENTIONAL

Study Start Date

2021-03-14

Study Completion Date

2025-10-31

Brief Summary

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Transurethral resection of bladder tumor (TURBT) is usually performed in a piecemeal technique. Tumor fragmentation and cell spilling could be responsible for high recurrence rates. Circulating tumor cells (CTCs) have been shown to be a prognostic predictor in disease progression in transitional cell carcinoma. In the current study the investigators aim to quantify CTCs in purging fluid and blood for recurrent intermediate risk bladder cancer during surgery for two different methods: TURBT and Plasma-kinetic vaporization of bladder tumor (PKVBT). Also correlations for recurrence will be investigated for the two different surgical methods.

Detailed Description

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Bladder cancer is the 9th most commonly diagnosed cancer in men worldwide, with a standardized incidence rate of 9.0 per 100,000 person-years for men and 2.2 per 100,000 person-years for women. Amongst any caner entity, bladder cancer is the most expensive cancer regarding follow-up and life-time treatment costs due to the high probability of recurrence. Up to 85% of patients initially present with non muscle-invasive bladder cancer (NMIBC). Progression to muscle-invasive bladder cancer (MIBC) is up to 10-20%. NMIBC is characterized by a high risk of recurrence after transurethral resection of bladder tumor (TURBT): the 1-yr recurrence rate is 15-61% and the 5-yr recurrence rate is 31-78%. These numbers represent the heterogeneity of NMIBC.

Against any existing oncological principle, during TURBT bladder tumors are resected in a piecemeal manner. This results in tumor fragmentation and floating cancer cells inside the bladder during surgery. These cells may have the ability to re-attach on and re-implant into the bladder wall and may be responsible for early disease recurrence which is commonly seen after initial surgery. It has been shown that tumor cells may access the circulatory system through cut vessels. Circulating tumor cells (CTCs) can be detected in up to 20% in T1 high grade disease and are commonly seen in metastasized disease. They have shown to be an independent predictor of disease progression and relapse in several studies and reflect biological aggressiveness.

In the current study the investigators want to quantify CTCs for recurrent intermediate risk transitional cell carcinoma in purging fluid and blood for two different surgical methods: TURBT and Plasma-kinetic vaporisation of bladder tumors (PKVBT). Also correlations for recurrence will be investigated for the two different surgical methods.

In 2 urological centers (LKH Hall, LKH Salzburg) participants with diagnosed intermediate risk recurrent transitional cell carcinoma of the bladder will be randomly enrolled for either TURBT or PKVBT. Before surgery CTCs will be analyzed in peripheral blood and purging fluid. (preoperative CTCs blood and purging fluid, morphological aspect of CTCs in purging fluid) After resection for TURBT and vaporization for PKVBT, a tumor ground biopsy will be taken for both groups. After coagluation, CTCs will again be drawn in peripheral blood (intraoperative CTCs blood). After completion of surgery an indwelling catheter is inserted and purging fluid is again analyzed (postoperative CTCs purging fluid, morphological aspect of CTCs in purging fluid). Blood is again taken on day 2 after surgery during the morning routine to assess CTCs after surgery (postoperative CTCs blood). Patients will be dismissed on earliest day 2 after surgery after indwelling catheter removal.

Recurrence will be assessed during follow-up by cystoscopic controls (From 3 to 36 months after surgery). If recurrence is detected the study is terminated. If no recurrence is detected up to 36 months after surgery, the study is likewise terminated.

Conditions

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Urinary Bladder Neoplasm Transitional Cell Carcinoma Urogenital Neoplasms Circulating Tumor Cell Neoplasms

Keywords

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Bladder cancer NMIBC CTC Circulating tumor cell Transurethral resection Plasma kinetic vaporization TURBT PKVB

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Pilot trial with parallel group design and comparison of 2 established techniques (TURBT, PKVB)
Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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TURBT (Transurethral Resection of Bladder Tumor)

For patients undergoing bipolar transurethral resection, bladder tumor is resected in a piecemeal manner.

Group Type ACTIVE_COMPARATOR

Bipolar transurethral resection of bladder tumor (TURBT)

Intervention Type DEVICE

Standard resection in piecemeal technique with standard bipolar cutting loop. (Storz medical, 27040 GP1)

PKVB (Plasma Kinetic Vaporization of Bladder Tumor)

For patients undergoing bipolar plasma kinetic vaporization of bladder tumor, bladder tumor is vaporized.

Group Type ACTIVE_COMPARATOR

Bipolar transurethral plasma kinetic vaporization of bladder tumor (PKVBT)

Intervention Type DEVICE

Vaporization of bladder tumor with standard bipolar vaporization electrode. (Storz medical, 27040 NB)

Interventions

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Bipolar transurethral resection of bladder tumor (TURBT)

Standard resection in piecemeal technique with standard bipolar cutting loop. (Storz medical, 27040 GP1)

Intervention Type DEVICE

Bipolar transurethral plasma kinetic vaporization of bladder tumor (PKVBT)

Vaporization of bladder tumor with standard bipolar vaporization electrode. (Storz medical, 27040 NB)

Intervention Type DEVICE

Eligibility Criteria

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Inclusion Criteria

* female and male patients
* recurrent bladder tumor
* preoperative cystoscopy
* CT or MRI scan of abdomen not older than 30 days prior to surgery without suspicion of advanced disease (MIBC, metastasis)
* max. non-invasive papillary tumor (pTa) staging in prior histology
* max. low grade grading in prior histology
* max. 5 lesions in actual cystoscopy (all \< 3cm)
* exophytic tumors
* transitional cell cancer of urinary bladder
* patient able to give consent
* signed consent form

Exclusion Criteria

* initial tumor
* flat lesion
* \> 3cm
* carcinoma in situ (CIS) in prior histology or suspicious CIS-finding in actual cystoscopy
* high grade grading in prior histology
* ≥ pT1 (tumor infiltration into subepithelial connective tissue) staging in prior histology
* \> 5 lesions
* different entity from transitional cell carcinoma of urinary bladder
* prior radiation
* emergency surgery
* prior indwelling catheter (extraction \< 1 week prior to surgery)
* pregnancy
* orthotopic neobladder
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Salzburger Landeskliniken

OTHER

Sponsor Role collaborator

University Teaching Hospital Hall in Tirol

OTHER

Sponsor Role lead

Responsible Party

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Gernot Ortner

Sub-Investigator

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Udo Nagele, MD, Prof.

Role: STUDY_CHAIR

Head of Department LKH Hall in Tirol

Lukas Lusuardi, MD, Prof.

Role: STUDY_CHAIR

Heas of Department LKH Salzburg

Locations

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LKH Hall in Tirol, Department of Urology and Andrology

Hall in Tirol, , Austria

Site Status

LKH Salzburg, Department of Urology and Andrology

Salzburg, , Austria

Site Status

Countries

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Austria

References

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Burger M, Catto JW, Dalbagni G, Grossman HB, Herr H, Karakiewicz P, Kassouf W, Kiemeney LA, La Vecchia C, Shariat S, Lotan Y. Epidemiology and risk factors of urothelial bladder cancer. Eur Urol. 2013 Feb;63(2):234-41. doi: 10.1016/j.eururo.2012.07.033. Epub 2012 Jul 25.

Reference Type BACKGROUND
PMID: 22877502 (View on PubMed)

Comperat E, Larre S, Roupret M, Neuzillet Y, Pignot G, Quintens H, Houede N, Roy C, Durand X, Varinot J, Vordos D, Rouanne M, Bakhri MA, Bertrand P, Jeglinschi SC, Cussenot O, Soulie M, Pfister C. Clinicopathological characteristics of urothelial bladder cancer in patients less than 40 years old. Virchows Arch. 2015 May;466(5):589-94. doi: 10.1007/s00428-015-1739-2. Epub 2015 Feb 20.

Reference Type BACKGROUND
PMID: 25697540 (View on PubMed)

Sylvester RJ, van der Meijden AP, Oosterlinck W, Witjes JA, Bouffioux C, Denis L, Newling DW, Kurth K. Predicting recurrence and progression in individual patients with stage Ta T1 bladder cancer using EORTC risk tables: a combined analysis of 2596 patients from seven EORTC trials. Eur Urol. 2006 Mar;49(3):466-5; discussion 475-7. doi: 10.1016/j.eururo.2005.12.031. Epub 2006 Jan 17.

Reference Type BACKGROUND
PMID: 16442208 (View on PubMed)

Babjuk M, Bohle A, Burger M, Capoun O, Cohen D, Comperat EM, Hernandez V, Kaasinen E, Palou J, Roupret M, van Rhijn BWG, Shariat SF, Soukup V, Sylvester RJ, Zigeuner R. EAU Guidelines on Non-Muscle-invasive Urothelial Carcinoma of the Bladder: Update 2016. Eur Urol. 2017 Mar;71(3):447-461. doi: 10.1016/j.eururo.2016.05.041. Epub 2016 Jun 17.

Reference Type BACKGROUND
PMID: 27324428 (View on PubMed)

Wilby D, Thomas K, Ray E, Chappell B, O'Brien T. Bladder cancer: new TUR techniques. World J Urol. 2009 Jun;27(3):309-12. doi: 10.1007/s00345-009-0398-9. Epub 2009 Mar 4.

Reference Type BACKGROUND
PMID: 19259684 (View on PubMed)

Rink M, Schwarzenbach H, Vetterlein MW, Riethdorf S, Soave A. The current role of circulating biomarkers in non-muscle invasive bladder cancer. Transl Androl Urol. 2019 Feb;8(1):61-75. doi: 10.21037/tau.2018.11.05.

Reference Type BACKGROUND
PMID: 30976570 (View on PubMed)

Engilbertsson H, Aaltonen KE, Bjornsson S, Kristmundsson T, Patschan O, Ryden L, Gudjonsson S. Transurethral bladder tumor resection can cause seeding of cancer cells into the bloodstream. J Urol. 2015 Jan;193(1):53-7. doi: 10.1016/j.juro.2014.06.083. Epub 2014 Jul 1.

Reference Type BACKGROUND
PMID: 24996129 (View on PubMed)

Zare R, Grabe M, Hermann GG, Malmstrom PU. Can routine outpatient follow-up of patients with bladder cancer be improved? A multicenter prospective observational assessment of blue light flexible cystoscopy and fulguration. Res Rep Urol. 2018 Oct 9;10:151-157. doi: 10.2147/RRU.S141314. eCollection 2018.

Reference Type BACKGROUND
PMID: 30349812 (View on PubMed)

Donat SM, North A, Dalbagni G, Herr HW. Efficacy of office fulguration for recurrent low grade papillary bladder tumors less than 0.5 cm. J Urol. 2004 Feb;171(2 Pt 1):636-9. doi: 10.1097/01.ju.0000103100.22951.5e.

Reference Type BACKGROUND
PMID: 14713776 (View on PubMed)

Gazzaniga P, de Berardinis E, Raimondi C, Gradilone A, Busetto GM, De Falco E, Nicolazzo C, Giovannone R, Gentile V, Cortesi E, Pantel K. Circulating tumor cells detection has independent prognostic impact in high-risk non-muscle invasive bladder cancer. Int J Cancer. 2014 Oct 15;135(8):1978-82. doi: 10.1002/ijc.28830. Epub 2014 Mar 13.

Reference Type BACKGROUND
PMID: 24599551 (View on PubMed)

Sievert KD, Amend B, Nagele U, Schilling D, Bedke J, Horstmann M, Hennenlotter J, Kruck S, Stenzl A. Economic aspects of bladder cancer: what are the benefits and costs? World J Urol. 2009 Jun;27(3):295-300. doi: 10.1007/s00345-009-0395-z. Epub 2009 Mar 7.

Reference Type BACKGROUND
PMID: 19271220 (View on PubMed)

Other Identifiers

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1

Identifier Type: -

Identifier Source: org_study_id