Improving P. Aeruginosa Detection With Breath-based Diagnostics (IMPACT-Breath)

NCT ID: NCT04735952

Last Updated: 2023-12-05

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

300 participants

Study Classification

OBSERVATIONAL

Study Start Date

2019-05-03

Study Completion Date

2024-12-31

Brief Summary

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The study is a breath biomarker validation study. It is anticipated that 300 patients with cystic fibrosis (CF) from 5 clinical sites in the USA will be enrolled. The study is funded by the US NIH and the US Cystic Fibrosis Foundation. Enrollment commenced in May 2019. Sputum, induced sputum, and oropharyngeal swabs will be collected and evaluated at each clinic as part of standard clinical practice. Excess sputum will be sent to Children's Colorado Hospital for molecular analysis. No swabs will be sent anywhere (other than the clinic from which they originate). Breath samples will be taken from all study participants.

Detailed Description

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AIM 1: Refine and validate volatile biomarkers in the breath of adult and pediatric CF patients for detecting established P. aeruginosa lung infections. For each expectorating subject (n ≥ 288; 5 centers), the diagnostic accuracy of the volatile biomarker panel will be tested, with sputum culture as the standard.

AIM 2: Quantify intra-subject breath variability of the target pediatric population. We will collect longitudinal breath samples for two years from P. aeruginosa-negative subjects (n ≥ 58; \~60% non-expectorating) at 4 pediatric CF clinical centers. We will measure intra-subject variance in the breath signatures of expectorating and non-expectorating subjects, with the latter being the target population for the clinical trial. Two to 8 breath samples will be collected per patient.

Conditions

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Cystic Fibrosis

Keywords

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Breath IMPACT

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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AIM 1

No-Intervention. Participants in this group will have 1 study visit only. During that visit, breath and sputum samples will be collected.

No interventions assigned to this group

AIM 2

No-Intervention. Participants in this group will have up to 8 study visits over a 2 year period. During the study visits, breath and sputum samples will be collected.

No interventions assigned to this group

Eligibility Criteria

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Inclusion Criteria

* Aim 1, Cross-Sectional


1. Male or female, ages 8 years and older
2. Diagnosis of CF based on sweat chloride ≥ 60 mmol/L and/or 2 known CF mutations
3. @ BC Children's Hospital only- Non-expectorators are eligible if having a clinically indicated OP swab.
4. FEV1 ≥ 30% predicted for spontaneous expectorators or FEV1 ≥ 40% predicted for subjects undergoing sputum induction
5. Either P. aeruginosa (Pa) negative or chronically infected with P. aeruginosa (Pa positive) as defined below:

a. P. aeruginosa negative: must meet one of the following criteria: i: No growth of Pa in previous 12 months and ≥ 4 consecutive Pa-negative cultures, OR ii: No history of Pa positive airway cultures (sputum, OP, Bronchoalveolar lavage) b. P. aeruginosa positive i: Over 50% of cultures positive and at least 2 cultures positive for Pa in previous 18 months
6. Willing to participate in and comply with the study procedures and willingness of the subject, parent or legally authorized representative to provide written informed consent.


1. Male or female, ages 8-16 years
2. Diagnosis of CF based on sweat chloride ≥ 60 mmol/L and/or 2 known CF mutations
3. @ BC Children's Hospital only- Non-expectorators are eligible if having a clinically indicated OP swab.
4. FEV1 ≥ 30% predicted for spontaneous expectorators or FEV1 ≥ 40% predicted for subjects undergoing sputum induction
5. P. aeruginosa negative, based on one of the following criteria:

1. No growth of Pa in previous 12 months and ≥ 4 consecutive Pa-negative cultures
2. No history of Pa positive airway cultures (sputum, OP, bronchoalveolar lavage)
6. Willing to participate in and comply with the study procedures and willingness of the subject, parent or legally authorized representative to provide written informed consent.


1. Male or female, ages 3-8 years
2. Diagnosis of CF based on sweat chloride ≥ 60 mmol/L and/or 2 known CF mutations 3. FEV1 ≥ 30%

4\. Unable to expectorate sputum 5. P. aeruginosa negative, based on one of the following criteria:

1. No growth of Pa in previous 12 months and ≥ 4 consecutive Pa-negative cultures
2. No history of Pa positive airway cultures (sputum, OP, bronchoalveolar lavage) 6. Willing to participate in and comply with the study procedures and willingness of the subject, parent or legally authorized representative to provide written informed consent.

Exclusion Criteria

1. Age \< 8 years
2. Intermittently infected with Pa
3. FEV1 \< 30%
4. History of lung transplant
5. Presence of a condition or abnormality that in the opinion of the Principal Investigator would compromise the safety of the subject or the quality of the data.

Aim 2, Longitudinal


1. Age \< 8 years
2. Intermittently or chronically infected with Pa
3. Unable to expectorate sputum or undergo sputum induction
4. FEV1 \< 30%
5. History of lung transplant
6. Presence of a condition or abnormality that in the opinion of the Principal Investigator would compromise the safety of the subject or the quality of the data.


1. Age \< 3 years
2. Intermittently or chronically infected with Pa
3. FEV1 \< 30%
4. History of lung transplant
5. Presence of a condition or abnormality that in the opinion of the Principal Investigator would compromise the safety of the subject or the quality of the data.
Minimum Eligible Age

3 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Arizona State University

OTHER

Sponsor Role collaborator

Children's Hospital Colorado

OTHER

Sponsor Role collaborator

National Heart, Lung, and Blood Institute (NHLBI)

NIH

Sponsor Role collaborator

Cystic Fibrosis Foundation

OTHER

Sponsor Role collaborator

University of British Columbia

OTHER

Sponsor Role lead

Responsible Party

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Jane Hill, PhD

Associate Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Jane E Hill, PhD

Role: PRINCIPAL_INVESTIGATOR

University of British Columbia

Locations

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Phoenix Children's Hospital

Phoenix, Arizona, United States

Site Status COMPLETED

Children's Hospital Colorado

Aurora, Colorado, United States

Site Status COMPLETED

National Jewish Health

Denver, Colorado, United States

Site Status COMPLETED

Dartmouth-Hitchcock Medical Center

Lebanon, New Hampshire, United States

Site Status NOT_YET_RECRUITING

Cincinnati Children's Hospital

Cincinnati, Ohio, United States

Site Status COMPLETED

BC Children's Hospital

Vancouver, British Columbia, Canada

Site Status RECRUITING

St. Paul's Hospital

Vancouver, British Columbia, Canada

Site Status RECRUITING

Countries

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United States Canada

Central Contacts

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Jane E Hill, PhD

Role: CONTACT

Phone: 778 879 5105

Email: [email protected]

Nina Nouribakikomarolya, PhD

Role: CONTACT

Phone: 778 325 5038

Email: [email protected]

Facility Contacts

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Brian O'Sullivan, MD

Role: primary

Kathy Phipps, BA

Role: backup

Jonathan Rayment, MD

Role: primary

Alam Lakhani

Role: backup

Bradley Quon, MD

Role: primary

Related Links

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http://impactbreath.org

Public access to study information and clinical site portal.

Other Identifiers

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Pro00043176

Identifier Type: OTHER

Identifier Source: secondary_id

HILL18A0

Identifier Type: OTHER_GRANT

Identifier Source: secondary_id

R56HL139846

Identifier Type: NIH

Identifier Source: secondary_id

View Link

331178/ H20-00071

Identifier Type: -

Identifier Source: org_study_id