Autophagy Markers in Endometrial Polyps

NCT ID: NCT04706000

Last Updated: 2023-12-06

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

30 participants

Study Classification

OBSERVATIONAL

Study Start Date

2021-01-08

Study Completion Date

2022-02-01

Brief Summary

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The aim of this research is to evaluate autophagy markers in patients with endometrial polyps

Detailed Description

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Endometrial polyp are benign formations that are frequently encountered in gynecology practice and have malignant potential. It may be asymptomatic in the clinic or may present with abnormal uterine bleeding. Although its pathophysiology has not been clearly revealed, there are many studies in the literature about it. However, the autophagy pathway emerges as a subject that has never been evaluated. In this study, the investigators would like to prospectively evaluate Beclin 1, LC3A / B and p62 markers in the autophagy pathway in endometrial polyp tissue samples by immunohistochemistry and reveal their importance.

Conditions

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Endometrial Polyp Autophagy

Keywords

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polyp endometrium beclin 1 LC3 p62

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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Study group

endometrial polyp

Beclin 1 gene

Intervention Type GENETIC

The determination of Beclin 1, LC3-II and p62 genes, which are evaluated by immunohistochemistry, at the protein level will be done by ELISA method in MSKU Medical Faculty Medical Biology Laboratory.

Control group

normal endometrium

Beclin 1 gene

Intervention Type GENETIC

The determination of Beclin 1, LC3-II and p62 genes, which are evaluated by immunohistochemistry, at the protein level will be done by ELISA method in MSKU Medical Faculty Medical Biology Laboratory.

Interventions

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Beclin 1 gene

The determination of Beclin 1, LC3-II and p62 genes, which are evaluated by immunohistochemistry, at the protein level will be done by ELISA method in MSKU Medical Faculty Medical Biology Laboratory.

Intervention Type GENETIC

Other Intervention Names

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LC3-II gene p62 gene

Eligibility Criteria

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Inclusion Criteria

* Patients over 35 years of age with a histopathologically confirmed diagnosis of endometrial polyp
* patients over the age of 35 whose histopathologically reported normal endometrial biopsy results.

Exclusion Criteria

* Are under the age of 35,
* those with cognitive dysfunction,
* diagnosed with any type of cancer
* who are under treatment for cancer,
* diabetes mellitus,
* hypertension,
* endometriosis,
* adenomyosis,
* chronic endometritis,
* have an acute or chronic illness,
* patients who have smoked and used alcohol in the last 10 years
Minimum Eligible Age

35 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

Yes

Sponsors

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Muğla Sıtkı Koçman University

OTHER

Sponsor Role lead

Responsible Party

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Burak Sezgin

Principal investigator M.D. Burak Sezgin

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Burak SEZGİN, MD

Role: PRINCIPAL_INVESTIGATOR

Muğla Sıtkı Koçman University

Locations

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Mugla Sıtkı Kocman University Faculty of Medicine

Muğla, , Turkey (Türkiye)

Site Status

Countries

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Turkey (Türkiye)

References

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Zhang J. Teaching the basics of autophagy and mitophagy to redox biologists--mechanisms and experimental approaches. Redox Biol. 2015;4:242-59. doi: 10.1016/j.redox.2015.01.003. Epub 2015 Jan 13.

Reference Type BACKGROUND
PMID: 25618581 (View on PubMed)

Zhan L, Yao S, Sun S, Su Q, Li J, Wei B. NLRC5 and autophagy combined as possible predictors in patients with endometriosis. Fertil Steril. 2018 Oct;110(5):949-956. doi: 10.1016/j.fertnstert.2018.06.028.

Reference Type BACKGROUND
PMID: 30316442 (View on PubMed)

Xie Z, Klionsky DJ. Autophagosome formation: core machinery and adaptations. Nat Cell Biol. 2007 Oct;9(10):1102-9. doi: 10.1038/ncb1007-1102.

Reference Type BACKGROUND
PMID: 17909521 (View on PubMed)

Wong M, Crnobrnja B, Liberale V, Dharmarajah K, Widschwendter M, Jurkovic D. The natural history of endometrial polyps. Hum Reprod. 2017 Feb;32(2):340-345. doi: 10.1093/humrep/dew307. Epub 2016 Dec 18.

Reference Type BACKGROUND
PMID: 27994000 (View on PubMed)

Zhang L, Liu Y, Xu Y, Wu H, Wei Z, Cao Y. The expression of the autophagy gene beclin-1 mRNA and protein in ectopic and eutopic endometrium of patients with endometriosis. Int J Fertil Steril. 2015 Jan-Mar;8(4):429-36. doi: 10.22074/ijfs.2015.4183. Epub 2015 Feb 7.

Reference Type RESULT
PMID: 25780525 (View on PubMed)

Other Identifiers

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03.09.20-08/01

Identifier Type: -

Identifier Source: org_study_id