Long-term COVID-19 Immune Response in a Vulnerable Neighbourhood in Argentina

NCT ID: NCT04673279

Last Updated: 2021-08-25

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

189 participants

Study Classification

OBSERVATIONAL

Study Start Date

2020-12-02

Study Completion Date

2021-08-20

Brief Summary

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Between June 10t h and July 1st, a cross-sectional design study in an Argentina slum, showed a prevalence based on immunoglobuling G-class (IgG) antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) of 53.4%. It remains unanswered whether natural infection produces sustained antibodies. The aim of this study is to evaluate the presence of IgG antibodies for Coronavirus disease 2019 (COVID-19) after 5 months in inhabitants of Barrio 31 who consented the Seroprevalence Study for COVID-19.

Detailed Description

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Background Between June 10t h and July 1st, a cross-sectional design study was carried out in an Argentina slum over people, selected from a probabilistic sample of households showed a prevalence based on IgG-class antibodies against SARS-CoV-2 of 53.4%. In regarding to the persistence of these antibodies, the early data brought hope that acquired immunity was possible. But some subsequent studies have suggested that immune protection might be short-lived. The new findings show that people who survive a COVID-19 infection continue to produce protective antibodies against key parts of the virus for at least three to four months after developing their first symptoms. In contrast, some other antibody types decline more quickly.

It remains unanswered whether natural infection produces a sustained immunity that is capable of establishing herd immunity. The other health problem that Latin America faces is dengue, whose transmission, like SARS-CoV-2, is greater in areas with high population density such as vulnerable neighborhoods. In this context, the occurrence of the two diseases implies a risk, particularly in regions with several dengue serotypes where secondary and tertiary infections have been demonstrated and dengue epidemiological surveillance has been affected by the saturation of health system.

Objectives The aim of this study is to evaluate the presence of IgG antibodies for COVID-19 after 5 months in inhabitants of this slum who consented the Seroprevalence Study for COVID-19, and to evaluate those factors associated with the persistence of positive antibodies. As a secondary objective, the presence of positive IgG for dengue will be evaluated.

Methods Cross sectional study. Population of the study were inhabitants of the slum: men and women form 14 years of age or older were included. People will be invited to participated and detection of antibodies will be performed with ta serological tests, an enzyme linked immunosorbent assay (ELISA) developed and validated in Argentina which detects antibodies against two viral antigens, trimeric spike and the receptor binding domain (RBD) of the spike protein. Blood sample will be collected in a capillary tube from a finger prick taken at the doorstep of each person. Samples will be processed and analyzed at the "Hospital de Niños Doctor Ricardo Gutierrez" Virology laboratory.

Blood sample collection and epidemiological data were collected, and entered in a secure database.

Sample Size The first seroprevalence study included 426 inhabitants of 14 years or more. Considering a persistence of antibodies of 30% with a precision of 5% for a confidence interval of 95% 184 inhabitants should be included. This sample will be selected by a proportionate stratified random sampling, considering the ten sectors in which the slum is divided.

Statistical analysis Descriptive statistics of the data will be carried out according to the variables obtained. Continuous variables will be expressed as mean and standard deviation, and categorical variables as proportions. A multiple logistic regression model will be performed to evaluate the factors associated with the persistence of positive antibodies for SARS-CoV-2.

Dengue seroprevalence is determined globally and by geographic sector. R software version 4.0.2 will be used.

Conditions

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Coronavirus Disease 2019

Study Design

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Observational Model Type

COHORT

Study Time Perspective

CROSS_SECTIONAL

Interventions

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serology

An enzyme linked immunosorbent assay (ELISA) developed and validated in Argentina which detects antibodies against two viral antigens, trimeric spike and the receptor binding domain (RBD) of the spike protein.

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

* Men and women form 14 years of age or older who were included in the seroprevalence study and who have positive antibodies against severe acute respiratory syndrome coronavirus 2

Exclusion Criteria

* deny consent
Minimum Eligible Age

14 Years

Maximum Eligible Age

90 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Hospital de Niños R. Gutierrez de Buenos Aires

OTHER

Sponsor Role collaborator

Ministerio de Salud GCBA

UNKNOWN

Sponsor Role collaborator

Hospital Italiano de Buenos Aires

OTHER

Sponsor Role lead

Responsible Party

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ALICIA MISTCHENKO

principal investigator

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Vanina Pagotto, MD MG

Role: STUDY_CHAIR

Hospital Italiano de Buenos Aires

Alicia Mistchenko, Phd

Role: PRINCIPAL_INVESTIGATOR

Hospital de Niños Ricardo Gutierrez

Silvana Figar, MD MG

Role: STUDY_CHAIR

Hospital Italiano de Buenos Aires

Andrea Gamarnick, Phd

Role: STUDY_CHAIR

National Council of Scientific and Technical Research, Argentina

Ana Maria Gomez Saldaño, MD MG

Role: STUDY_CHAIR

Salud Comunitaria Ministerio de Salud del Gobierno de la Ciudad de Buenos Aires

Lorena Luna

Role: STUDY_CHAIR

Salud Comunitaria Ministerio de Salud del Gobierno de la Ciudad de Buenos Aires

Julieta Salto

Role: STUDY_CHAIR

Salud Comunitaria Ministerio de Salud del Gobierno de la Ciudad de Buenos Aires

Magdalena Wagner Manslau

Role: STUDY_CHAIR

Salud Comunitaria Ministerio de Salud del Gobierno de la Ciudad de Buenos Aires

Fernan Quiroz, MD MG

Role: STUDY_DIRECTOR

Ministerio de Salud del Gobierno de la Ciudad de Buenos Aires

Locations

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Hospital de Niños Ricardo Gutierrez

Buenos Aires, Buenos Aires F.D., Argentina

Site Status

Countries

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Argentina

References

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Jordan RE, Adab P, Cheng KK. Covid-19: risk factors for severe disease and death. BMJ. 2020 Mar 26;368:m1198. doi: 10.1136/bmj.m1198. No abstract available.

Reference Type BACKGROUND
PMID: 32217618 (View on PubMed)

Long QX, Tang XJ, Shi QL, Li Q, Deng HJ, Yuan J, Hu JL, Xu W, Zhang Y, Lv FJ, Su K, Zhang F, Gong J, Wu B, Liu XM, Li JJ, Qiu JF, Chen J, Huang AL. Clinical and immunological assessment of asymptomatic SARS-CoV-2 infections. Nat Med. 2020 Aug;26(8):1200-1204. doi: 10.1038/s41591-020-0965-6. Epub 2020 Jun 18.

Reference Type BACKGROUND
PMID: 32555424 (View on PubMed)

Kellam P, Barclay W. The dynamics of humoral immune responses following SARS-CoV-2 infection and the potential for reinfection. J Gen Virol. 2020 Aug;101(8):791-797. doi: 10.1099/jgv.0.001439.

Reference Type BACKGROUND
PMID: 32430094 (View on PubMed)

Wilder-Smith A, Tissera H, Ooi EE, Coloma J, Scott TW, Gubler DJ. Preventing Dengue Epidemics during the COVID-19 Pandemic. Am J Trop Med Hyg. 2020 Aug;103(2):570-571. doi: 10.4269/ajtmh.20-0480. Epub 2020 Jun 15. No abstract available.

Reference Type BACKGROUND
PMID: 32539912 (View on PubMed)

Cardoso MR, Cousens SN, de Goes Siqueira LF, Alves FM, D'Angelo LA. Crowding: risk factor or protective factor for lower respiratory disease in young children? BMC Public Health. 2004 Jun 3;4:19. doi: 10.1186/1471-2458-4-19.

Reference Type BACKGROUND
PMID: 15176983 (View on PubMed)

Related Links

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http://dx.doi.org/10.3348/kjr.2020.0181

2019 Novel Coronavirus (COVID-19) Pneumonia with Hemoptysis as the Initial Symptom: CT and Clinical Features

http://dx.doi.org/10.1101/2020.07.21.20159178

SARS-CoV-2 infection induces sustained humoral immune responses in convalescent patients following symptomatic COVID-19

https://www.medrxiv.org/content/10.1101/2020.07.14.20153858v2.full.pdf

Community-level SARS-CoV-2 Seroprevalence Survey in urban slum dwellers of Buenos Aires City, Argentina: a participatory research.

https://doi.org/10.1038/s41467-020-18271-5

Reductions in commuting mobility correlate with geographic differences in SARS-CoV-2 prevalence in New York City

https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0008716

The COVID-19 pandemic should not jeopardize dengue control

Other Identifiers

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3545

Identifier Type: -

Identifier Source: org_study_id

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