An Amniotic Membrane Injection Comparing Two Doses (1 mL and 2 mL Injection) and a Placebo (Sterile Saline) in the Treatment of Osteoarthritis of the Knee

NCT ID: NCT04612023

Last Updated: 2021-02-15

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

PHASE2

Total Enrollment

90 participants

Study Classification

INTERVENTIONAL

Study Start Date

2020-11-18

Study Completion Date

2022-07-02

Brief Summary

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The purpose of this study is to determine the dose effect of a single injectable acellular amniotic membrane derived allograft for the treatment of knee osteoarthritis and to confirm whether the use of 2 mL of the same amniotic injection offers a statistically significant advantage over the 1 mL injection when compared to a placebo.

Detailed Description

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It is a prospective, double blinded, Randomized Controlled Trial study. Data will be prospectively collected of all injectable acellular amniotic membrane derived allograft knee performed at our institute (1 vs 2 mL).

90 subjects will be enrolled in the study. Subjects will be randomized 1:1:1 in treatment arms.

It compares the dose affect of an amniotic membrane allograft between a 1 mL dose and a 2 mL dose when compared to a placebo of sterile saline in the treatment of osteoarthritis of the knee. This will be offered as a free pain management alternative to patients who meet the inclusion criteria. The results will be determined by validated patient-reported outcome tools (KOOS, VAS, and WOMAC questionnaires) and physical examinations taken before the injection, one month after the injection, three months after the injection, six months after the injection, and one year after the injection. There will be a phone call 24 hours after the injection for every patient participating in the study regarding any potential adverse events.

Conditions

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Osteoarthritis, Knee

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Data will be prospectively collected on all injectable acellular amniotic membrane derived allograft knee performed at our institute. (FDA regulated - HCT/P regulation)

Inclusion criteria will be all patients age between 21 and 80 years with a diagnosis of osteoarthritis (OA) defined as Grade 1 to 3 on the Kellgren Lawrence grading scale.

Methods for collecting data will be through validated patient-reported outcome tools that the patient will complete pre-injection and at specified time intervals after injection: 30 days, 90 days, 180 days and 365 days.

Patient will receive a phone call 24 hours after the injection to monitor any immediate potential adverse events.
Primary Study Purpose

TREATMENT

Blinding Strategy

TRIPLE

Participants Caregivers Investigators
Assistants not part of the study used a randomizer generating software to randomly assign a patient a dose. The randomly assigned dose was put in a sealed envelope labeled with the corresponding number and was recorded. Prior to the injection, a medical assistant not involved in the study retrieves the envelope to determine the dose, prepares the injection, then seals the syringe in black tape before giving it to the research staff who then performs the injection.

Study Groups

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1 mL NyDYN Injection

30 patients (out of 90) will be blind to and randomly assigned to a 1 mL NyDYN injection.

Group Type ACTIVE_COMPARATOR

Acellular Amniotic Membrane Derived Allograft Injection (NuDYN)

Intervention Type OTHER

Injectable acellular amniotic membrane derived allograft tissue (NuDYN) through routine follow-up with physical examination, pain, function and quality of life measurements and compare dose effects (1 mL versus 2 mL of NuDYN injection) to a placebo (sterile saline) in the treatment of knee osteoarthritis. Injection is minimally invasive and is FDA regulated (HCT/P regulation).

2 mL NuDYN Injection

30 patients (out of 90) will be blind to and randomly assigned to a 2 mL NyDYN injection.

Group Type ACTIVE_COMPARATOR

Acellular Amniotic Membrane Derived Allograft Injection (NuDYN)

Intervention Type OTHER

Injectable acellular amniotic membrane derived allograft tissue (NuDYN) through routine follow-up with physical examination, pain, function and quality of life measurements and compare dose effects (1 mL versus 2 mL of NuDYN injection) to a placebo (sterile saline) in the treatment of knee osteoarthritis. Injection is minimally invasive and is FDA regulated (HCT/P regulation).

Placebo of Sterile Saline

30 patients (out of 90) will be blind to and randomly assigned to a 2 mL dose of sterile saline.

Group Type PLACEBO_COMPARATOR

Acellular Amniotic Membrane Derived Allograft Injection (NuDYN)

Intervention Type OTHER

Injectable acellular amniotic membrane derived allograft tissue (NuDYN) through routine follow-up with physical examination, pain, function and quality of life measurements and compare dose effects (1 mL versus 2 mL of NuDYN injection) to a placebo (sterile saline) in the treatment of knee osteoarthritis. Injection is minimally invasive and is FDA regulated (HCT/P regulation).

Interventions

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Acellular Amniotic Membrane Derived Allograft Injection (NuDYN)

Injectable acellular amniotic membrane derived allograft tissue (NuDYN) through routine follow-up with physical examination, pain, function and quality of life measurements and compare dose effects (1 mL versus 2 mL of NuDYN injection) to a placebo (sterile saline) in the treatment of knee osteoarthritis. Injection is minimally invasive and is FDA regulated (HCT/P regulation).

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Patient is a candidate for non-surgical intervention of the knee
* Patient must be between the ages of 21 and 80 years old
* Patient must have a diagnosis of osteoarthritis (OA) of the knee defined as grade 1 to 3 on the Kellgren-Lawrence grading scale

Exclusion Criteria

* Patient has a diagnosis of osteoarthritis with a Kellgren-Lawrence grade of 4
* Patient has a BMI greater than 40 kg/m2, active infection at the injection site, symptomatic OA of the contralateral knee or of either hip that is not responsive to acetaminophen (Tylenol) and requires other therapy
* Patient has rheumatoid arthritis, psoriatic arthritis or diagnosis with any other disorder that is the primary source of their knee pain
* Patient has an autoimmune disease or known history of having Acquired Immunodeficiency Syndrome (AIDS) or HIV
* Patient has any of the following treatments to the target knee within 12 weeks prior to screening
* Intra-articular hyaluronic acid (HA) injection
* Steroid or platelet rich plasma (PRP) injection
* Use of any investigational drug, device, or biologic
* Patient had or is planning to have major surgery or arthroscopy in the target knee within 26 weeks of treatment
* Patient has a history of partial or total knee arthroplasty
* Patient has undergone immunotherapy or chemotherapy in the last 5 years, prior radiation at the site, or is currently taking a narcotic medication for any reason
* Patient is pregnant or plans to become pregnant within 365 days of treatment
* Patient has any significant medical condition that would interfere with protocol evaluation and participation
* Patient is a recipient of worker's compensation
* Patient is a current prisoner
Minimum Eligible Age

21 Years

Maximum Eligible Age

80 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Illinois Center for Orthopaedic Research and Education

OTHER

Sponsor Role lead

Responsible Party

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Eyal Ginesin

Orthopedic Surgeon / Fellow Researcher

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Nikil Chari

Role: STUDY_CHAIR

Research Assistant

Jacob Barnhart

Role: STUDY_CHAIR

Research Assistant

Locations

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ICORE

Westmont, Illinois, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Eyal Ginesin, MD

Role: CONTACT

6309292249

Ronak M Patel, MD

Role: CONTACT

6309292249

Facility Contacts

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Ronak M Patel, MD

Role: primary

630-929-2249

Eyal Ginesin, MD

Role: backup

6309292249

References

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Riboh JC, Saltzman BM, Yanke AB, Cole BJ. Human Amniotic Membrane-Derived Products in Sports Medicine: Basic Science, Early Results, and Potential Clinical Applications. Am J Sports Med. 2016 Sep;44(9):2425-34. doi: 10.1177/0363546515612750. Epub 2015 Nov 19.

Reference Type BACKGROUND
PMID: 26585668 (View on PubMed)

Vines JB, Aliprantis AO, Gomoll AH, Farr J. Cryopreserved Amniotic Suspension for the Treatment of Knee Osteoarthritis. J Knee Surg. 2016 Aug;29(6):443-50. doi: 10.1055/s-0035-1569481. Epub 2015 Dec 18.

Reference Type BACKGROUND
PMID: 26683979 (View on PubMed)

Other Identifiers

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8212

Identifier Type: -

Identifier Source: org_study_id

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