Streptococcus Mutans Cariogenicity by Real-time PCR in High Caries Risk Patients

NCT ID: NCT04566120

Last Updated: 2023-12-13

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

37 participants

Study Classification

INTERVENTIONAL

Study Start Date

2021-01-01

Study Completion Date

2023-02-01

Brief Summary

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In patients with high caries risk, will the use of cinnamon extract or chlorohexidine based mouthwashes have an effect on the percentage of recovery and cariogenicity of Streptococcus mutans (SM) detected by real-time Polymerase Chain Reaction (PCR) over one month follow up

Detailed Description

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This clinical study will be held in the clinic of conservative dentistry department, Faculty of Dentistry, Cairo University. The operator in charge will be Mostafa Mahmoud El-Ghazali. Patients will be examined and selected according to inclusion and exclusion criteria, the purpose and method of the study will be explaned to the selected patients, written informed consent will be obtained from the patients for participation in the study. In the first visit, ADA caries risk assessment model will be done. Participants will be divided into two groups according to the tested mouthwashes.

All patients will be instructed not to have breakfast and to refrain any oral hygiene measures on the day of sample collection to avoid the influence of food consumption and contamination on the composition of saliva. The patient will sit in an erect position on the dental chair and will be given paraffin block to chew. The stimulated saliva will be collected after 2 minutes of paraffin chewing in sterile containers held near the mouth. The containers will be properly labeled. The study will be carried over a period of one month, the saliva samples for each patient will be collected on the first dental visit. These samples will be then sent to the laboratory for microbiological assessment. Patients will be provided with mouthwashes according to the tested groups and they will be instructed to use it 2 times per day for 1 minute each time and no eating or drinking permitted for a minimum of 30 minutes post rinsing. Each patient will be given the same instructions with respect to oral hygiene measures. They will be advised to continue on same diet and will be instructed not to change their dietary habits. Patients will come after 2 weeks for salivary samples collection and samples will be sent to the laboratory for microbiological assessment. After treatment continuity for another 2 weeks, patients will come for the last visit to collect the salivary samples which will be sent to the laboratory for microbiological assessment.

Conditions

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Streptococcus Mutans High Caries Risk Patients

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

TRIPLE

Participants Investigators Outcome Assessors

Study Groups

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Cinnamon extract mouthwash

Participants will be exposed to cinnamon extract mouthwash and will be instructed to use 10 ml of cinnamon extract mouthwash 2 times per day for 1 minute; such a regimen will be continued for one month.

Group Type EXPERIMENTAL

cinnamon extract mouthwash

Intervention Type DRUG

cinnamon extract mouthwash

Chlorohexidine based mouthwash

Participants will be exposed to chlorohexidine based mouthwash. and will be instructed to use 10 ml of 0.12% chlorhexidine based mouthwash 2 times per day for 1 minute; such a regimen will be continued for one month.

Group Type ACTIVE_COMPARATOR

chlorohexidine based mouthwash

Intervention Type DRUG

chlorohexidine based mouthwash

Interventions

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cinnamon extract mouthwash

cinnamon extract mouthwash

Intervention Type DRUG

chlorohexidine based mouthwash

chlorohexidine based mouthwash

Intervention Type DRUG

Other Intervention Names

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Natural Product Mouthwash Synthetic Mouthwash

Eligibility Criteria

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Inclusion Criteria

* Adults will be recruited in this study, all the volunteers participated in this experiment will be healthy looking with free medical history.
* Age range 20-50 years
* High caries risk patients according to ADA caries risk assessment model.
* High plaque index (\>score 2)
* Non-smoking patients.
* Patients with normal salivary rate (0.3-0.4 ml/min).

Exclusion Criteria

* Patients with a compromised medical history.
* Participants with low caries risk.
* Patients with severe or active periodontal disease.
* Participants with a history of allergy to any of the drugs or chemicals used in the study.
* Patients on any antibiotics during the past month
* Smoking patients.
* Patients with abnormal salivary rate.
* Pregnant female patients
Minimum Eligible Age

20 Years

Maximum Eligible Age

50 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Cairo University

OTHER

Sponsor Role lead

Responsible Party

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Mostafa Mahmoud Mohamed Kamel El-Ghazali

Principle Investigator

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Mostafa El-Ghazali

Role: PRINCIPAL_INVESTIGATOR

Assistant Lecturer

Locations

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Cairo Uniuversity

Giza, , Egypt

Site Status

Countries

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Egypt

Other Identifiers

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oper131

Identifier Type: -

Identifier Source: org_study_id