NAFLD and Liver Fibrosis in Obese Adolescents

NCT ID: NCT04561804

Last Updated: 2020-09-24

Basic Information

• Recruitment Status: COMPLETED
• Total Enrollment: 100 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2018-12-01
• Study Completion Date: 2019-12-01

Brief Summary

Nonalcoholic fatty liver disease (NAFLD) is a chronic liver disease resulting from excessive fat accumulation in the liver. Due to its close association with obesity, it has become the most common liver disease in children in the United States. NAFLD can result in progressive fibrosis and lead to end-stage liver disease. Best practices in management of pediatric NAFLD are not clearly defined.

Our aim is to clarify the natural history of NAFLD in obese children after weight loss surgery compare to lifestyle intervention. Our secondary aim is to investigate the added value of elastography for the screening and diagnosis of NASH with fibrosis.

Detailed Description

The study population will consist of obese adolescents, male and female, age 7-18, that will go through weight loss reduction surgery (WLS) in the Obesity Clinic at Tel Aviv Sourasky Medical Center. A control group matched for age and sex will consist of patients that will go through conventional treatment (see below) at the Obesity Clinic in Dana Dwek Children's Hospital.

Written informed consent form will be signed by the participant before enrollment Patient Population Patients with a diagnosed primary liver disease (autoimmune liver disease, metabolic liver disease, Wilson's disease, alpha 1 antitrypsin deficiency), patients treated with medications known to induce steatosis (such as valproate, amiodarone or prednisone), and patients with hepatic virus infections or history of parenteral nutrition were excluded from the study.

Study Design and measurements This clinical trial aims to asses the effect of bariatric surgery and dietary intervention on hepatic fibrosis in obese pediatric population with NAFLD (Clinical Trial Registration- MOH_2017-06-07_000470, https://my.health.gov.il/CliniTrials/Pages/MOH_2017-06-07_000470.aspx). At the initial visit, data were collected on socioeconomic parameters, lifestyle, birth details, and medical, family, and social histories. All patients underwent anthropometric measures (height, weight and BMI) and a physical examination focused on obesity-related conditions. Laboratory evaluation included liver enzyme profile, lipid profile, glucose, insulin, and HbA1C. Liver fibrosis was estimated by Shearwave elastography (Supersonic) and categorized into 4 levels, F0-F4, according to liver stiffness (measured by kPa), as recently demonstrated elsewhere [9]. Liver steatosis was calculated by a hepatorenal index (HRI) and divided into 3 levels of severity. All measurements were taken by a single experienced radiologist (MW), who was blinded to the results of other parameters of the patients. A multidisciplinary team included a gastroenterologist, hepatologists, a registered dietitian (RD) and a psychologist. All participants received nutritional recommendations (see below) and general recommendation for a healthy lifestyle (regular engagement in daily physical activity and reduction of screen time). Compliance with the dietary guideline was reviewed by a RD on a biweekly basis with a 3-day food questionnaire (2 weekdays and 1 day of the weekend). Blood tests and elastography were repeated after 3 months of intervention.

Determination of the BMI percentiles for age and gender was based on WHO growth charts. "Obesity" was defined as a BMI >95th percentile . Abnormal glucose metabolism included taking a hypoglycemic medication or having an elevated homeostatic model assessment index of insulin resistance, glucose, or HBA1c. Hypertriglyceridemia and hypercholesterolemia were defined as a serum level >95 percentile for age and sex. Hypertension was diagnosed as systolic and/or diastolic blood pressure ≥95 percentile for age and sex. Patients with clinical suspicion of obstructive sleep apnea (OSA) were diagnosed by polysomnography that was conducted by the hospital's sleep specialists. Socioeconomic status was defined according to parents' years of education.

The dietary intervention The participants received nutritional recommendations for a low carbohydrate, low glycemic load, and isocaloric diet. The diet was composed of carbohydrates (CHO;30-40%), fats (35-50%), and proteins (20-25%), and was tailored to individual preferences and calorie requirements. The number of CHO, protein and fat servings was determined based on the recommended total energy requirements for age, calculated on the basis of dietary reference intake (DRI). Participants were not instructed to restrict calories, but to reduce carbohydrate based on their glycemic load. High glycemic index (GI) carbohydrate intake (refined grains, potatoes, sweet and salted snacks and sugar sweetened beverages) was completely restricted, low GI carbohydrates (non-starchy vegetables, legumes, nuts,) were allowed and some low /moderate GI carbohydrate were allowed but limited such as fruits and whole grain bread.

The subjects were instructed about appropriate food choices, and each participant was provided a diet information booklet containing food list, sample menus and recipes

Statistical Analyses Descriptive statistics were examined for all variables. Continuous variables were expressed as median with interquartile range (IQR) when they were not normally distributed and as mean ± standard deviation (SD) for normally distributed variables. Categorical variables were presented as number and percentage. Categorical variables were compared by the McNamer test and continuous and ordinal variables by the Wilcoxon test. The Fischer test was used when the McNamer test was not applicable for some variables. The Pearson correlation and simple linear regression analysis were performed to examine bivariate associations between fibrosis and metabolic and nutritional parameters. The Wilcoxon signed rank test was applied to compare the difference between steatosis, fibrosis, and metabolic parameters between the 2 time points (baseline and 3-month follow-up). A P level <0.05 was considered statistically significant. All statistical tests were 2-sided. The statistical analysis was performed with SPSS (IBM SPSS statistics, version 22, IBM Corp. Armonk, NY, USA, 2013.).

Conditions

Liver Fibrosis; Liver Steatosis; Weight Loss

Study Design

• Observational Model Type: CASE_ONLY
• Study Time Perspective: PROSPECTIVE

Study Groups

LISESTYLE INTERVENTION

LOW CARB LOW GLYCEMIC LOAS DIET

No interventions assigned to this group

WEGHT LOSS SURGERY

SLEEVE OR MINBYPASS SURGERY

laparoscopic sleeve gastrectomy (LSG) and laparoscopic single anastomosis gastric bypass (LSAGB)

Intervention Type: PROCEDURE

The participants will receive nutritional recommendations for a low carbohydrate, low glycemic load, and isocaloric diet. The diet will be composed of carbohydrates (CHO;35%), fats (40-50%), and proteins (20-25%), and will be tailored to individual preferences and calorie requirements. The number of CHO, protein and fat servings will be determined based on the recommended total energy requirements for age, calculated on the basis of dietary reference intake (DRI). Participants will not be instructed to restrict calories, but to reduce carbohydrate based on their glycemic load.

The subjects will be instructed about appropriate food choices, and each subject was provided a diet information booklet containing food list, sample menus and recipes

Interventions

laparoscopic sleeve gastrectomy (LSG) and laparoscopic single anastomosis gastric bypass (LSAGB)

The participants will receive nutritional recommendations for a low carbohydrate, low glycemic load, and isocaloric diet. The diet will be composed of carbohydrates (CHO;35%), fats (40-50%), and proteins (20-25%), and will be tailored to individual preferences and calorie requirements. The number of CHO, protein and fat servings will be determined based on the recommended total energy requirements for age, calculated on the basis of dietary reference intake (DRI). Participants will not be instructed to restrict calories, but to reduce carbohydrate based on their glycemic load.

The subjects will be instructed about appropriate food choices, and each subject was provided a diet information booklet containing food list, sample menus and recipes

Intervention Type: PROCEDURE

Other Intervention Names

Dietary intervention

Eligibility Criteria

Inclusion Criteria

• Age 13-18 years.
• No other chronic disease except the metabolic syndrome.
• Not taking any medication

Exclusion Criteria

• Children that are known to suffer from genetic/metabolic disorders that can cause steatosis- Wilson disease, hypothyroidism, autoimmune hepatitis, Alpha 1 antitrypsin.
• Children infected with HBV or HCV, HAV.
• Children that use medications that can cause liver steatosis- amiodarone, MTX, Corticosteroids
• Children with significant ethanol consumption ->10 gr per day for women and >20gr per day for men.
• Pregnant women.
• Obesity due to syndromes or monogenic disease

General withdraw criteria:

• If the participant withdraws his consent.

• Accepts Healthy Volunteers: No

Sponsors

Tel-Aviv Sourasky Medical Center

OTHER_GOV

Sponsor Role: lead

Responsible Party

michal roll

Prof Shlomi Cohen

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

sHLOMI Cohen, prof

Role: PRINCIPAL_INVESTIGATOR

Dana Dwek Children Hospital

Locations

Pediatric Gastroenterology

Tel Aviv, , Israel

Countries

Israel

References

Moran-Lev H, Cohen S, Webb M, Yerushalmy-Feler A, Amir A, Gal DL, Lubetzky R. Higher BMI predicts liver fibrosis among obese children and adolescents with NAFLD - an interventional pilot study. BMC Pediatr. 2021 Sep 3;21(1):385. doi: 10.1186/s12887-021-02839-1.

Reference Type: DERIVED

PMID: 34479517 (View on PubMed)

Study Documents

Document Type: Study Protocol

Israel Ministry of Health registration number

View Document

Other Identifiers

0097-17-TLV

Identifier Type: -

Identifier Source: org_study_id