Trial Outcomes & Findings for ADEQUATE Advanced Diagnostics for Enhanced Quality of Antibiotic Prescription (NCT NCT04547556)

NCT ID: NCT04547556

Last Updated: 2024-10-15

Results Overview

Days alive out of hospital (superiority endpoint), within 14 days after study enrolment

Recruitment status

TERMINATED

Study phase

NA

Target enrollment

185 participants

Primary outcome timeframe

Day 1 - Day 14

Results posted on

2024-10-15

Participant Flow

Participant milestones

Participant milestones
Measure
Intervention
BioFire: A molecular rapid syndromic testing platform, using the following panels: * BioFire FilmArray Respiratory Panel 2.1 plus (RP2.1plus) * BioFire FilmArray Pneumonia Panel plus (PP)
Standard of Care
Standard of care diagnostics
Overall Study
STARTED
92
93
Overall Study
COMPLETED
92
93
Overall Study
NOT COMPLETED
0
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Race and Ethnicity were not collected from any participant.

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Intervention
n=92 Participants
BioFire: A molecular rapid syndromic testing platform, using the following panels: * BioFire FilmArray Respiratory Panel 2.1 plus (RP2.1plus) * BioFire FilmArray Pneumonia Panel plus (PP)
Standard of Care
n=93 Participants
Standard of care diagnostic testing
Total
n=185 Participants
Total of all reporting groups
Age, Continuous
66.5 years
n=92 Participants
67 years
n=93 Participants
67 years
n=185 Participants
Sex: Female, Male
Female
46 Participants
n=92 Participants
45 Participants
n=93 Participants
91 Participants
n=185 Participants
Sex: Female, Male
Male
46 Participants
n=92 Participants
48 Participants
n=93 Participants
94 Participants
n=185 Participants
Race and Ethnicity Not Collected
0 Participants
Race and Ethnicity were not collected from any participant.
Region of Enrollment
Serbia
88 participants
n=92 Participants
91 participants
n=93 Participants
179 participants
n=185 Participants
Region of Enrollment
Belgium
3 participants
n=92 Participants
2 participants
n=93 Participants
5 participants
n=185 Participants
Region of Enrollment
Hungary
1 participants
n=92 Participants
0 participants
n=93 Participants
1 participants
n=185 Participants

PRIMARY outcome

Timeframe: Day 1 - Day 14

Population: Days alive out of hospital

Days alive out of hospital (superiority endpoint), within 14 days after study enrolment

Outcome measures

Outcome measures
Measure
Intervention
n=92 Participants
BioFire: A molecular rapid syndromic testing platform, using the following panels: * BioFire FilmArray Respiratory Panel 2.1 plus (RP2.1plus) * BioFire FilmArray Pneumonia Panel plus (PP)
Standard of Care
n=93 Participants
Standard of Care diagnostic testing
Days Alive Out of Hospital (Superiority Endpoint)
10.7 days
Standard Deviation 5.3
9.7 days
Standard Deviation 5.9

PRIMARY outcome

Timeframe: Day 1 - Day 14

Population: Number of antibiotic treatment days

Days on Therapy (DOT) with antibiotics (superiority endpoint), within 14 days after study enrolment

Outcome measures

Outcome measures
Measure
Intervention
n=92 Participants
BioFire: A molecular rapid syndromic testing platform, using the following panels: * BioFire FilmArray Respiratory Panel 2.1 plus (RP2.1plus) * BioFire FilmArray Pneumonia Panel plus (PP)
Standard of Care
n=93 Participants
Standard of Care diagnostic testing
Days on Therapy (DOT) With Antibiotics (Superiority Endpoint)
6.7 days
Standard Deviation 4.6
7 days
Standard Deviation 4.7

PRIMARY outcome

Timeframe: Day 1 - Day 30

* For initially non-admitted patients: any admission or death * For initially hospitalized patients: any readmission, ICU admission \>= 24 hours after hospitalization, or death

Outcome measures

Outcome measures
Measure
Intervention
n=92 Participants
BioFire: A molecular rapid syndromic testing platform, using the following panels: * BioFire FilmArray Respiratory Panel 2.1 plus (RP2.1plus) * BioFire FilmArray Pneumonia Panel plus (PP)
Standard of Care
n=93 Participants
Standard of Care diagnostic testing
Adverse Outcome (Non-inferiority Safety Endpoint)
9 Participants
18 Participants

SECONDARY outcome

Timeframe: Day 1 - Day 30

Population: This outcome will not be analysed since direct costs and indirect costs could not be properly collected. Cost effective algorithms might change with factors as the disease incidence changes, performance of other diagnostic tests or its combination. As these other factors are also dependent on country and vary over time, the data as collected in the trial is insufficient to reliably report on cost-effectiveness in general

* Cost of healthcare within 30 days after enrolment, including hospital and ICU days, utilisation of non-hospital services and cost of anti-infective and concomitant medication * Cost of workdays lost within 30 days, including days for childcare

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: Day 1

Population: Data collected includes: sample type (upper and lower respiratory tract samples for diagnostic intervention but also blood cultures and urinary antigen tests for standard of care if applicable), type of diagnostic test, specific results. Data is also collected regarding the time elapsed until the results were received. Results are final for this trial and later on will be compared with pediatric trial results NCT04781530.

Proportion of participants with an identified respiratory pathogen in both study groups on randomisation day samples. Data refers to the number of participants so in case more than one microorganism is detected in the same sample (co-detection) or same result in more than one sample, it is still counted as one participant.

Outcome measures

Outcome measures
Measure
Intervention
n=92 Participants
BioFire: A molecular rapid syndromic testing platform, using the following panels: * BioFire FilmArray Respiratory Panel 2.1 plus (RP2.1plus) * BioFire FilmArray Pneumonia Panel plus (PP)
Standard of Care
n=93 Participants
Standard of Care diagnostic testing
Microbiological Results Obtained as Standard of Care and With the Diagnostic Intervention
58 Participants
31 Participants

SECONDARY outcome

Timeframe: Day 1 - Day 14

Population: Final analysis needs to integrate data from both the adult and the pediatric protocol and to adjust to local guidelines per country as well as risk factors and comorbidities. Data collected includes: antibiotics, antivirals and antifungals recording if initial, switch or addition to ongoing therapy as well as route of administration, dosing interval and start and stop date.

Proportion of participants on non-first-line anti-infective regimens (as defined by local guidelines). For this report, results are defined as non first-line if the choice includes a third or fourth generation cephalosporin or a carbapenem but analysis needs to be adjusted to baseline risk factors, comorbidities, local guidelines and time to de escalation.

Outcome measures

Outcome measures
Measure
Intervention
n=92 Participants
BioFire: A molecular rapid syndromic testing platform, using the following panels: * BioFire FilmArray Respiratory Panel 2.1 plus (RP2.1plus) * BioFire FilmArray Pneumonia Panel plus (PP)
Standard of Care
n=93 Participants
Standard of Care diagnostic testing
Empirical Antibiotics Based on Antimicrobial Agent Categories
30 Participants
33 Participants

SECONDARY outcome

Timeframe: Day 1 - Day 14

Population: Final analysis needs to integrate data from both the adult and the pediatric protocol and to adjust to local guidelines per country as well as risk factors and comorbidities. Data collected includes: antibiotics, antivirals and antifungals recording if initial, switch or addition to ongoing therapy as well as route of administration, dosing interval and start and stop date.

For this report is presented the number of patients where the antimicrobial was switched to narrower spectrum.

Outcome measures

Outcome measures
Measure
Intervention
n=92 Participants
BioFire: A molecular rapid syndromic testing platform, using the following panels: * BioFire FilmArray Respiratory Panel 2.1 plus (RP2.1plus) * BioFire FilmArray Pneumonia Panel plus (PP)
Standard of Care
n=93 Participants
Standard of Care diagnostic testing
Antibiotic Type Switches and De-escalation Based on Antimicrobial Agent Categories
11 Participants
15 Participants

SECONDARY outcome

Timeframe: >7 days after randomisation

Population: Hospitalised participants. Samples obtained as standard of care during hospital stay. Detection of multidrug resistant microorganism was recorded.

Proportion of hospitalised participants with detection of cephalosporin-, carbapenem- or chinolone-resistant Enterobacteriaceae on any standard of care samples \>7 days after randomisation comparing diagnostic intervention arm and standard of care arm.

Outcome measures

Outcome measures
Measure
Intervention
n=27 Participants
BioFire: A molecular rapid syndromic testing platform, using the following panels: * BioFire FilmArray Respiratory Panel 2.1 plus (RP2.1plus) * BioFire FilmArray Pneumonia Panel plus (PP)
Standard of Care
n=32 Participants
Standard of Care diagnostic testing
Detection of Antimicrobial Resistance (Carriage or Infection) Related to the Diagnostic Intervention Results Compared to Standard of Care and Impact on Antimicrobial Stewardship Guidelines and Prevention of Hospital Acquired Infections.
0 Participants
0 Participants

SECONDARY outcome

Timeframe: Day 1 - Day 30

Population: Hospitalised participants. Data collected includes start and stop date of individual or cohort isolation. Isolation measures in the current cohort were related to SARS-CoV-2 infection and not to the detection of multidrug resistant bacteria.

Hours in individual or cohort isolation in hospitalised participants comparing the 2 groups

Outcome measures

Outcome measures
Measure
Intervention
n=27 Participants
BioFire: A molecular rapid syndromic testing platform, using the following panels: * BioFire FilmArray Respiratory Panel 2.1 plus (RP2.1plus) * BioFire FilmArray Pneumonia Panel plus (PP)
Standard of Care
n=32 Participants
Standard of Care diagnostic testing
Impact on Decisions Regarding Isolation Measures Related to Test Result.
0 hours in isolation
Standard Deviation 0
0 hours in isolation
Standard Deviation 0

Adverse Events

Intervention

Serious events: 5 serious events
Other events: 0 other events
Deaths: 5 deaths

Standard of Care

Serious events: 14 serious events
Other events: 3 other events
Deaths: 11 deaths

Serious adverse events

Serious adverse events
Measure
Intervention
n=92 participants at risk
BioFire: A molecular rapid syndromic testing platform, using the following panels: * BioFire FilmArray Respiratory Panel 2.1 plus (RP2.1plus) * BioFire FilmArray Pneumonia Panel plus (PP)
Standard of Care
n=93 participants at risk
Standard of Care testing
Respiratory, thoracic and mediastinal disorders
Acute respiratory failure
0.00%
0/92 • From the time of signing the informed consent through study completion (day-30 for the main study or 6 months for the extension study).
Standard definitions of adverse events and device related events.
1.1%
1/93 • Number of events 1 • From the time of signing the informed consent through study completion (day-30 for the main study or 6 months for the extension study).
Standard definitions of adverse events and device related events.
Cardiac disorders
Cardiac arrest
4.3%
4/92 • Number of events 4 • From the time of signing the informed consent through study completion (day-30 for the main study or 6 months for the extension study).
Standard definitions of adverse events and device related events.
10.8%
10/93 • Number of events 10 • From the time of signing the informed consent through study completion (day-30 for the main study or 6 months for the extension study).
Standard definitions of adverse events and device related events.
Injury, poisoning and procedural complications
Femur fracture
0.00%
0/92 • From the time of signing the informed consent through study completion (day-30 for the main study or 6 months for the extension study).
Standard definitions of adverse events and device related events.
1.1%
1/93 • Number of events 1 • From the time of signing the informed consent through study completion (day-30 for the main study or 6 months for the extension study).
Standard definitions of adverse events and device related events.
Respiratory, thoracic and mediastinal disorders
Pulmonary artery thrombosis
0.00%
0/92 • From the time of signing the informed consent through study completion (day-30 for the main study or 6 months for the extension study).
Standard definitions of adverse events and device related events.
1.1%
1/93 • Number of events 1 • From the time of signing the informed consent through study completion (day-30 for the main study or 6 months for the extension study).
Standard definitions of adverse events and device related events.
Respiratory, thoracic and mediastinal disorders
Respiratory failure
0.00%
0/92 • From the time of signing the informed consent through study completion (day-30 for the main study or 6 months for the extension study).
Standard definitions of adverse events and device related events.
1.1%
1/93 • Number of events 1 • From the time of signing the informed consent through study completion (day-30 for the main study or 6 months for the extension study).
Standard definitions of adverse events and device related events.
Renal and urinary disorders
Chronic kidney disease
1.1%
1/92 • Number of events 1 • From the time of signing the informed consent through study completion (day-30 for the main study or 6 months for the extension study).
Standard definitions of adverse events and device related events.
0.00%
0/93 • From the time of signing the informed consent through study completion (day-30 for the main study or 6 months for the extension study).
Standard definitions of adverse events and device related events.

Other adverse events

Other adverse events
Measure
Intervention
n=92 participants at risk
BioFire: A molecular rapid syndromic testing platform, using the following panels: * BioFire FilmArray Respiratory Panel 2.1 plus (RP2.1plus) * BioFire FilmArray Pneumonia Panel plus (PP)
Standard of Care
n=93 participants at risk
Standard of Care testing
Cardiac disorders
Cardiac failure
0.00%
0/92 • From the time of signing the informed consent through study completion (day-30 for the main study or 6 months for the extension study).
Standard definitions of adverse events and device related events.
1.1%
1/93 • Number of events 1 • From the time of signing the informed consent through study completion (day-30 for the main study or 6 months for the extension study).
Standard definitions of adverse events and device related events.
Respiratory, thoracic and mediastinal disorders
Pulmonary embolism
0.00%
0/92 • From the time of signing the informed consent through study completion (day-30 for the main study or 6 months for the extension study).
Standard definitions of adverse events and device related events.
1.1%
1/93 • Number of events 1 • From the time of signing the informed consent through study completion (day-30 for the main study or 6 months for the extension study).
Standard definitions of adverse events and device related events.
Respiratory, thoracic and mediastinal disorders
COVID 19
0.00%
0/92 • From the time of signing the informed consent through study completion (day-30 for the main study or 6 months for the extension study).
Standard definitions of adverse events and device related events.
1.1%
1/93 • Number of events 1 • From the time of signing the informed consent through study completion (day-30 for the main study or 6 months for the extension study).
Standard definitions of adverse events and device related events.

Additional Information

Dr Cristina Prat Aymerich

UMC Utrecht

Phone: +31631118128

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place