Trial Outcomes & Findings for Effect of Biologicals on Alternative Functions of Eosinophils in Severe Asthma (NCT NCT04520165)

NCT ID: NCT04520165

Last Updated: 2024-10-04

Results Overview

Remission was defined as patients combining after the instauration of the biotherapy: no oral corticosteroids, no exacerbation, ACQ lower than 1.5 and/or asthma control test (ACT) greater than 19 and, a FEV1 greater or equal to 80% predicted and/or an improvement of FEV1 greater or equal to 10% and a blood eosinophil count lower than 300 cells/microliter. Markers of remission in the sputum cell profile were investigated.

Recruitment status

ACTIVE_NOT_RECRUITING

Target enrollment

52 participants

Primary outcome timeframe

1 year follow-up

Results posted on

2024-10-04

Participant Flow

This observational study was performed at the CHU of Liege, Belgium, between 2014 and 2021

Inclusion criteria included a diagnosis of asthma defined by the Global Initiative for Asthma (http://ginasthma.org/), severe asthma was defined according to European Respiratory Society (ERS)/ American Thoracic Society (ATS) criteria 1. The treatment was stable for all patients at the time of the sampling and the time between baseline and the next evaluation was in average one year

Participant milestones

Participant milestones
Measure
Remission /Not
Fifty-two severe asthmatics initiated with an anti-IL-5 (51 with Mepolizumab and one with Reslizumab) were recruited from our asthma clinic. Remission was defined as patients combining after the instauration of the biotherapy: no oral corticosteroids, no exacerbation, ACQ lower than 1.5 and/or asthma control test (ACT) greater than 19 and, a FEV1 greater than 80% predicted and/or an improvement of FEV1 at least 10% from baseline and a blood eosinophil count lower than 300 cells/microliter.
Overall Study
STARTED
52
Overall Study
COMPLETED
52
Overall Study
NOT COMPLETED
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Effect of Biologicals on Alternative Functions of Eosinophils in Severe Asthma

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Remission /Not
n=52 Participants
Fifty-two severe asthmatics initiated with an anti-IL-5 (51 with Mepolizumab and one with Reslizumab) were recruited from our asthma clinic. Remission was defined as patients combining after the instauration of the biotherapy: no oral corticosteroids, no exacerbation, ACQ lower than 1.5 and/or asthma control test (ACT) greater than 19 and, a FEV1 greater than 80% predicted and/or an improvement of FEV1 at least 10% from baseline and a blood eosinophil count lower than 300 cells/microliter.
Age, Categorical
<=18 years
0 Participants
n=5 Participants
Age, Categorical
Between 18 and 65 years
52 Participants
n=5 Participants
Age, Categorical
>=65 years
0 Participants
n=5 Participants
Age, Continuous
48 years
STANDARD_DEVIATION 18 • n=5 Participants
Sex: Female, Male
Female
29 Participants
n=5 Participants
Sex: Female, Male
Male
23 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
0 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
0 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
52 Participants
n=5 Participants
Region of Enrollment
Belgium
52 participants
n=5 Participants

PRIMARY outcome

Timeframe: 1 year follow-up

Population: To compare groups, baseline characteristics were compared with Mann-Whitney U test

Remission was defined as patients combining after the instauration of the biotherapy: no oral corticosteroids, no exacerbation, ACQ lower than 1.5 and/or asthma control test (ACT) greater than 19 and, a FEV1 greater or equal to 80% predicted and/or an improvement of FEV1 greater or equal to 10% and a blood eosinophil count lower than 300 cells/microliter. Markers of remission in the sputum cell profile were investigated.

Outcome measures

Outcome measures
Measure
Remission
n=11 Participants
Remission was defined as patients combining after the instauration of the biotherapy: no oral corticosteroids, no exacerbation, ACQ lower than 1.5 and/or asthma control test (ACT) greater than 19 and, a FEV1 greater than 80% predicted and/or an improvement of FEV1 at least 10% from baseline and a blood eosinophil count lower than 300 cells/microliter.
No Remission
n=41 Participants
patients that did not fulfilled the remission criteria
Presence of Prognostic Markers of Remission in the Sputum Cell Compartment or Not
Sputum eosinophil count
494000 cells/g
Interval 235000.0 to 1663000.0
156000 cells/g
Interval 16000.0 to 362000.0
Presence of Prognostic Markers of Remission in the Sputum Cell Compartment or Not
Sputum neutrophil count
798000 cells/g
Interval 437000.0 to 3689000.0
943000 cells/g
Interval 544000.0 to 2604000.0
Presence of Prognostic Markers of Remission in the Sputum Cell Compartment or Not
Sputum macrophage count
526000 cells/g
Interval 296000.0 to 988000.0
193000 cells/g
Interval 92000.0 to 504000.0
Presence of Prognostic Markers of Remission in the Sputum Cell Compartment or Not
Sputum lymphocyte count
32000 cells/g
Interval 7000.0 to 144000.0
4000 cells/g
Interval 0.0 to 31000.0

PRIMARY outcome

Timeframe: 1 year follow-up

Population: To compare groups, baseline characteristics were compared with Mann-Whitney U test

Remission was defined as patients combining after the instauration of the biotherapy: no oral corticosteroids, no exacerbation, ACQ lower than 1.5 and/or asthma control test (ACT) greater than 19 and, a FEV1 greater or equal to 80% predicted and/or an improvement of FEV1 greater or equal to 10% and a blood eosinophil count lower than 300 cells/microliter. Markers ( inflammatory mediators) of remission in the sputum were investigated.

Outcome measures

Outcome measures
Measure
Remission
n=11 Participants
Remission was defined as patients combining after the instauration of the biotherapy: no oral corticosteroids, no exacerbation, ACQ lower than 1.5 and/or asthma control test (ACT) greater than 19 and, a FEV1 greater than 80% predicted and/or an improvement of FEV1 at least 10% from baseline and a blood eosinophil count lower than 300 cells/microliter.
No Remission
n=41 Participants
patients that did not fulfilled the remission criteria
Presence of Prognostic Markers (Mediators) of Remission in the Sputum or Not
Eotaxin-1
71 pg/ml
Interval 56.0 to 204.0
54 pg/ml
Interval 0.0 to 87.0
Presence of Prognostic Markers (Mediators) of Remission in the Sputum or Not
TSLP
3.2 pg/ml
Interval 2.4 to 6.5
2.2 pg/ml
Interval 1.0 to 3.6
Presence of Prognostic Markers (Mediators) of Remission in the Sputum or Not
IL-5
11.5 pg/ml
Interval 3.5 to 22.2
2.7 pg/ml
Interval 1.3 to 4.8
Presence of Prognostic Markers (Mediators) of Remission in the Sputum or Not
EPX
183000 pg/ml
Interval 129000.0 to 342000.0
48000 pg/ml
Interval 30000.0 to 99000.0
Presence of Prognostic Markers (Mediators) of Remission in the Sputum or Not
IgE
1200 pg/ml
Interval 400.0 to 2400.0
400 pg/ml
Interval 300.0 to 600.0

SECONDARY outcome

Timeframe: 1 year follow-up

Population: To compare groups, baseline characteristics were compared with Mann-Whitey U test

ACT improvement = greater than 19 post-treatment, EPX = Eosinophil peroxidase in the sputum supernatant

Outcome measures

Outcome measures
Measure
Remission
n=11 Participants
Remission was defined as patients combining after the instauration of the biotherapy: no oral corticosteroids, no exacerbation, ACQ lower than 1.5 and/or asthma control test (ACT) greater than 19 and, a FEV1 greater than 80% predicted and/or an improvement of FEV1 at least 10% from baseline and a blood eosinophil count lower than 300 cells/microliter.
No Remission
n=41 Participants
patients that did not fulfilled the remission criteria
is EPX in the Sputum of Prognostic Marker of ACT Improvement in the Sputum or Not ?
129 ng/ml
Interval 47.0 to 241.0
48 ng/ml
Interval 30.0 to 148.0

SECONDARY outcome

Timeframe: 1 year follow-up

Population: To compare groups, baseline characteristics were compared with Mann-Whitney U test

improvement of FEV1 = at least 10% post-treatment IL-5 = interleukin 5 measured in the sputum supernatant

Outcome measures

Outcome measures
Measure
Remission
n=11 Participants
Remission was defined as patients combining after the instauration of the biotherapy: no oral corticosteroids, no exacerbation, ACQ lower than 1.5 and/or asthma control test (ACT) greater than 19 and, a FEV1 greater than 80% predicted and/or an improvement of FEV1 at least 10% from baseline and a blood eosinophil count lower than 300 cells/microliter.
No Remission
n=41 Participants
patients that did not fulfilled the remission criteria
Is IL-5 in the Sputum a Prognosis Marker for Improvement of FEV1 Post-treatment or Not ?
9 pg/ml
Interval 2.0 to 22.0
3 pg/ml
Interval 1.0 to 5.0

Adverse Events

Remission /Not

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Dr Florence Schleich

CHU-Uliege

Phone: 003243667881

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place