A Study to Identify Breast Cancer (IDBC)

NCT ID: NCT04495244

Last Updated: 2020-07-31

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

600 participants

Study Classification

OBSERVATIONAL

Study Start Date

2019-01-24

Study Completion Date

2022-12-31

Brief Summary

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Detecting cancer as early as possible is key to achieving positive outcomes in response to diagnosis and treatment. The current project is aimed at validating a novel blood-based breast cancer identification test (Syantra DX Breast Cancer) that has been developed by Syantra Inc. Syantra DX Breast Cancer measures gene expression signatures in whole blood, and has been retrospectively demonstrated in 780 samples. The test uses proprietary algorithms to analyse gene expression characteristics from a novel multi-biomarker panel, and then classify a sample as positive or negative for breast cancer. Based upon test performance in a retrospective environment, a prospective validation study is being proposed.

The primary objective of this study is to validate Syantra DX Breast Cancer methodology and biomarker panel using prospective samples, well categorized by diagnostic imaging scores, pathology outcomes, and subject characteristics.

Detailed Description

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The current project is aimed at validating Syantra DX Breast Cancer in a UK population. Results of the UK study will be combined with those from additional sites, including those in Canada, with additional samples expected from the United States and South Korea. Syantra DX Breast Cancer measures gene expression signatures in whole blood, and has been retrospectively demonstrated in 780 samples. The test uses proprietary algorithms to analyse gene expression characteristics from a novel multi-biomarker panel, and then classify a sample as positive or negative for breast cancer. Based upon test performance in a retrospective environment, a prospective validation study is being proposed, and is described below.

The study seeks to evaluate performance of Syantra DX Breast Cancer in prospective samples and to optimize test methodology and biomarkers. Key to performance of the study is recruitment of women who have received an abnormal mammography report, and have been classified under the UK Royal College of Radiologists Breast Group (RCRBG) scoring system in categories 3 - 5. This will support the ability to employ the test for breast cancer detection in a screening/call-back environment. To complete the study cohort, additional women will be recruited from the population who have normal or benign mammography results (RCRBG category 1 and 2), and/or a recent normal physical breast exam, with no history of breast cancer. Blood samples will be acquired from all women following completion of an informed consent procedure.

For those with an abnormal mammogram, blood will be drawn before a biopsy is performed. All sample information will be de-identified, and the samples will be processed and analysed according to Syantra DX Breast Cancer standard operating procedures. These procedures include extracting sample RNA and aliquoting for storage in a manner that maintains sample anonymity. All processing and analysis will be conducted in a blind environment without knowing the associated RCRBG or pathology outcomes for any samples. Only after testing is complete will sample classifications be revealed. Syantra DX Breast Cancer results will then be compared to RCRBG, biopsy and pathology data to determine concordance, and statistics will be calculated. If or when biomarker panel or test methodology is modified during the performance of the study, samples will be reanalysed with updated test parameters. This reanalysis will make use of a de-identified aliquot of sample RNA to ensure that testing is maintained in a blind environment.

There is an optional part of the study procedure that is included as 'additional research'. A specific question seeking approval for the additional research is included on the study consent form. For individuals who agree to participate in the additional research, material extracted from their blood sample will be stored for additional investigations.

Conditions

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Breast Cancer Female

Keywords

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Detection, blood test, clinical assay

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Investigational Population

Suspected Invasive Breast Cancer (any size) or DCIS (pre-invasive) are of particular interest. Women with abnormal screening mammograms (R3-5) recalled for further investigation.

Inclusion criteria

* Women with suspected Invasive Breast Cancer (tumours of any size and/or DCIS).
* Individuals with an abnormal or suspicious screening mammogram recalled for further evaluation.
* Suspected Invasive Breast Cancer (tumours of any size and/or DCIS).
* Willing to give written Informed Consent and provide whole blood samples
* Age 30-75 years

Exclusion criteria

* Breast surgery within the previous 12 months (for any reason) or recent breast biopsy (including needle core biopsy)
* Inoperable (T4 category) or inflammatory breast cancer
* Previous history of cancer previously at any site
* Previous history of breast cancer
* Concomitant or other concurrent anti-cancer therapy
* Male
* No histopathological diagnosis

Syantra DX Breast Cancer

Intervention Type DIAGNOSTIC_TEST

Blood test for detecting the presence of breast cancer.

Borderline (Atypica and LCIS) Population

Women with borderline pathological B3 lesions (suspected atypia Ductal or Lobular and benign proliferative disease without DCIS or invasion) Inclusion criteria

* Abnormal screening mammogram with suspected benign breast disease or proliferative changes
* Willing to give Written Informed Consent and provide whole blood samples
* Aged 30-75 years

Exclusion criteria

* Existing cancer diagnosis
* Male
* Breast surgery within the previous 12 months (for any reason) or recent breast biopsy (including needle core biopsy)
* Previous history of any cancer
* Previous history of breast cancer
* Concomitant or other concurrent anti-cancer therapy

Syantra DX Breast Cancer

Intervention Type DIAGNOSTIC_TEST

Blood test for detecting the presence of breast cancer.

Control Population

Healthy Controls Inclusion criteria

* Normal breast examination - No cancer detected/suspected by physical exam, diagnostic radiology or screening mammography
* Willing to give Written Informed Consent and provide whole blood samples
* Aged 30-75 years

Exclusion criteria

* Cancer diagnosis
* Male
* Breast surgery within the previous 12 months (for any reason) or recent breast biopsy (including needle core biopsy)
* Previous history of any cancer
* Concomitant or other concurrent anti-cancer therapy

Syantra DX Breast Cancer

Intervention Type DIAGNOSTIC_TEST

Blood test for detecting the presence of breast cancer.

Interventions

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Syantra DX Breast Cancer

Blood test for detecting the presence of breast cancer.

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

* Aged 30-75 years
* Normal breast examination - No cancer detected/suspected by physical exam, diagnostic radiology or screening mammography
* Women with suspected Invasive Breast Cancer
* Individuals with an abnormal or suspicious mammogram
* Abnormal screening mammogram with suspected benign breast disease or proliferative changes

Exclusion Criteria

* Cancer diagnosis
* Male
* Breast surgery within the previous 12 months (for any reason) or recent breast biopsy (including needle core biopsy)
* Previous history of any cancer
* Concomitant or other concurrent anti-cancer therapy
* Inoperable or inflammatory breast cancer
Minimum Eligible Age

30 Years

Maximum Eligible Age

75 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

Yes

Sponsors

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Syantra Inc.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Nigel Bundred, MD

Role: PRINCIPAL_INVESTIGATOR

Manchester University NHS Foundation Trust

Cliona Kirwin, MD

Role: STUDY_DIRECTOR

Manchester University NHS Foundation Trust

Locations

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Wythenshawe Hospital, Southmoor Road

Manchester, , United Kingdom

Site Status RECRUITING

Countries

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United Kingdom

Central Contacts

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Hayley Brooks

Role: CONTACT

Phone: 0161 291 4045

Email: [email protected]

Linda Bailey

Role: CONTACT

Phone: 0161 291 4492

Email: [email protected]

Facility Contacts

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Hayley Brooks

Role: primary

Other Identifiers

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241391

Identifier Type: -

Identifier Source: org_study_id