Trial Outcomes & Findings for Ph 3 Efficacy and Safety of B-VEC for the Treatment of DEB (NCT NCT04491604)

NCT ID: NCT04491604

Last Updated: 2023-02-17

Results Overview

The primary wound was defined as a responder wound that met either of the following conditions: * Complete wound healing on Week 22 and Week 24, or * Complete wound healing on Week 24 and Week 26. For subjects with missing primary wound healing data, a multiple imputation approach (10 repliates) was used. The total numbers of primary wounds with complete healing for B-VEC and Placebo presented below were the average of those from the multiple imputation replicates, and therefore, they would not be whole numbers (integers).

Recruitment status

COMPLETED

Study phase

PHASE3

Target enrollment

31 participants

Primary outcome timeframe

26 weeks post-baseline

Results posted on

2023-02-17

Participant Flow

Unit of analysis: Wounds

Participant milestones

Participant milestones
Measure
All Participants ("Topical Beremagene Geperpavec (B-VEC)", and "Placebo")
Each subject provided one pair of primary target wounds, with one wound from each pair randomized to be treated with B-VEC and the other wound with placebo.
Overall Study
STARTED
31 62
Overall Study
B-VEC
31 31
Overall Study
Placebo
31 31
Overall Study
COMPLETED
28 56
Overall Study
NOT COMPLETED
3 6

Reasons for withdrawal

Reasons for withdrawal
Measure
All Participants ("Topical Beremagene Geperpavec (B-VEC)", and "Placebo")
Each subject provided one pair of primary target wounds, with one wound from each pair randomized to be treated with B-VEC and the other wound with placebo.
Overall Study
Withdrawal by Subject
3

Baseline Characteristics

Ph 3 Efficacy and Safety of B-VEC for the Treatment of DEB

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
All Participants ("Topical Beremagene Geperpavec (B-VEC)", and "Placebo")
n=31 Participants
Each subject provided one pair of primary target wounds, with one wound from each pair randomized to be treated with B-VEC and the other wound with placebo.
Age, Continuous
17.2 years
STANDARD_DEVIATION 10.70 • n=5 Participants
Age, Customized
<= 12 years
10 Participants
n=5 Participants
Age, Customized
> 12 and <= 18 years
9 Participants
n=5 Participants
Age, Customized
> 18 years
12 Participants
n=5 Participants
Sex: Female, Male
Female
11 Participants
n=5 Participants
Sex: Female, Male
Male
20 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
16 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
15 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
Race (NIH/OMB)
American Indian or Alaska Native
5 Participants
n=5 Participants
Race (NIH/OMB)
Asian
6 Participants
n=5 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=5 Participants
Race (NIH/OMB)
Black or African American
0 Participants
n=5 Participants
Race (NIH/OMB)
White
20 Participants
n=5 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=5 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
Region of Enrollment
United States
31 participants
n=5 Participants
Primary Wound Area(cm^2) B-VEC
14.354 cm^2
STANDARD_DEVIATION 12.6930 • n=5 Participants
Primary Wound Area(cm^2) Placebo
15.565 cm^2
STANDARD_DEVIATION 12.1315 • n=5 Participants

PRIMARY outcome

Timeframe: 26 weeks post-baseline

Population: The intent-to-treat (ITT) population included subjects whose primary wounds were randomized regardless of whether they received randomized treatment or not.

The primary wound was defined as a responder wound that met either of the following conditions: * Complete wound healing on Week 22 and Week 24, or * Complete wound healing on Week 24 and Week 26. For subjects with missing primary wound healing data, a multiple imputation approach (10 repliates) was used. The total numbers of primary wounds with complete healing for B-VEC and Placebo presented below were the average of those from the multiple imputation replicates, and therefore, they would not be whole numbers (integers).

Outcome measures

Outcome measures
Measure
B-VEC
n=31 Wounds
Topical gel of non-integrating, replication-incompetent HSV-1 expressing the human collagen VII protein
Placebo
n=31 Wounds
Matching masked inactive topical gel
Primary Wound With Complete Wound Healing (100% Wound Closure) on Weeks 22 and 24 or Weeks 24 and 26
20.9 number of wounds with complete healing
6.7 number of wounds with complete healing

SECONDARY outcome

Timeframe: 12 weeks post-baseline

Population: The intent-to-treat (ITT) population included subjects whose primary wounds were randomized regardless of whether they received randomized treatment or not.

The primary wound was defined as a responder wound that met either of the following conditions: * Complete wound healing on Week 8 and Week 10, or * Complete wound healing on Week 10 and Week 12. For subjects with missing primary wound healing data, a multiple imputation approach (10 repliates) was used. The total numbers of primary wounds with complete healing for B-VEC and Placebo presented below were the average of those from the multiple imputation replicates, and therefore, they would not be whole numbers (integers).

Outcome measures

Outcome measures
Measure
B-VEC
n=31 Wounds
Topical gel of non-integrating, replication-incompetent HSV-1 expressing the human collagen VII protein
Placebo
n=31 Wounds
Matching masked inactive topical gel
Primary Wound With Complete Wound Healing (100% Wound Closure) on Weeks 8 and 10 or Weeks 10 and 12
21.9 number of wounds with complete healing
6.1 number of wounds with complete healing

SECONDARY outcome

Timeframe: 26 weeks post-baseline

Population: The intent-to-treat (ITT) population included subjects whose primary wounds were randomized regardless of whether they received randomized treatment or not. For pain severity, only subjects ages 6 and above were evaluated (n=27). The analyses were based on observed data where missing data were not imputed, therefore the numbers of available data for analyses could be less than the total.

Changes from baseline at Weeks 22, 24, and 26 in primary wound pain severity (visual analog scale (VAS)) for ages 6 and above subjects. The Visual Analog Scale scores from 0 (no pain) to 10 (the worst possible pain). Negative values in changes from baseline mean improvement in pain severity.

Outcome measures

Outcome measures
Measure
B-VEC
n=27 Participants
Topical gel of non-integrating, replication-incompetent HSV-1 expressing the human collagen VII protein
Placebo
n=27 Participants
Matching masked inactive topical gel
Primary Wound Pain Severity (Visual Analog Scale (VAS)) Change for Ages 6 and Above Subjects at Weeks 22, 24, and 26.
Week 22
-0.88 score on a scale of 0 to 10
Standard Deviation 2.346
-0.71 score on a scale of 0 to 10
Standard Deviation 2.476
Primary Wound Pain Severity (Visual Analog Scale (VAS)) Change for Ages 6 and Above Subjects at Weeks 22, 24, and 26.
Week 24
-0.64 score on a scale of 0 to 10
Standard Deviation 2.325
-0.08 score on a scale of 0 to 10
Standard Deviation 2.548
Primary Wound Pain Severity (Visual Analog Scale (VAS)) Change for Ages 6 and Above Subjects at Weeks 22, 24, and 26.
Week 26
-0.63 score on a scale of 0 to 10
Standard Deviation 2.123
-0.38 score on a scale of 0 to 10
Standard Deviation 2.871

Adverse Events

All Participants ("Topical Beremagene Geperpavec (B-VEC)", and "Placebo")

Serious events: 3 serious events
Other events: 17 other events
Deaths: 0 deaths

Serious adverse events

Serious adverse events
Measure
All Participants ("Topical Beremagene Geperpavec (B-VEC)", and "Placebo")
n=31 participants at risk
Each subject provided one pair of primary target wounds, with one wound from each pair randomized to be treated with B-VEC and the other wound with placebo. Due to the 'split-person'/intrasubject design, each subject received both B-VEC and Placebo. Therefore, the safety assessments were reported at subject level, but not per intervention.
Infections and infestations
Cellulitis
3.2%
1/31 • Number of events 1 • 6 months
Gastrointestinal disorders
Diarrhoea
3.2%
1/31 • Number of events 1 • 6 months
Blood and lymphatic system disorders
Anaemia
3.2%
1/31 • Number of events 2 • 6 months
Investigations
Blood culture positive
3.2%
1/31 • Number of events 1 • 6 months

Other adverse events

Other adverse events
Measure
All Participants ("Topical Beremagene Geperpavec (B-VEC)", and "Placebo")
n=31 participants at risk
Each subject provided one pair of primary target wounds, with one wound from each pair randomized to be treated with B-VEC and the other wound with placebo. Due to the 'split-person'/intrasubject design, each subject received both B-VEC and Placebo. Therefore, the safety assessments were reported at subject level, but not per intervention.
Skin and subcutaneous tissue disorders
Pruritus
9.7%
3/31 • Number of events 4 • 6 months
Skin and subcutaneous tissue disorders
Erythema
6.5%
2/31 • Number of events 2 • 6 months
Skin and subcutaneous tissue disorders
Rash
6.5%
2/31 • Number of events 2 • 6 months
General disorders
Chills
9.7%
3/31 • Number of events 3 • 6 months
Neoplasms benign, malignant and unspecified (incl cysts and polyps)
Squamous cell carcinoma
9.7%
3/31 • Number of events 4 • 6 months
Respiratory, thoracic and mediastinal disorders
Cough
6.5%
2/31 • Number of events 2 • 6 months
Respiratory, thoracic and mediastinal disorders
Rhinorrhoea
6.5%
2/31 • Number of events 2 • 6 months

Additional Information

Dr. Hubert Chen, MD, Senior Vice President of Clinical Development

Krystal Biotech

Phone: (412) 586-5830

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place