Spartan Cube CYP2C19 Method Comparison Study

NCT ID: NCT04473586

Last Updated: 2020-07-16

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

425 participants

Study Classification

INTERVENTIONAL

Study Start Date

2020-02-05

Study Completion Date

2020-03-06

Brief Summary

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The aim of the study is to demonstrate concordance (percent agreement) between results produced by the Spartan CYP2C19 system and bi-directional sequencing. In addition, this study will evaluate the concordance of buccal samples stored prior to running on the Spartan Cube CYP2C19 system and bi-directional sequencing.

Detailed Description

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Sample holding time - two parts

1. Sample tested in less than one-hour

a.In the first part, buccal samples will be collected from subjects and tested using the Spartan Cube CYP2C19 system. These samples will be run immediately (within 1 h) after collection from the subject (\<1 h sample holding time). All results will be compared to bi-directional sequencing to determine percent agreement.
2. Sample tested greater than 21 hours a.In the second part (sample holding portion), samples will be tested using the Spartan Cube CYP2C19 product. These samples will be run after storage at ambient temperature. All results will be compared to bi-directional sequencing to determine the percent agreement.

Conditions

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Genotyping Techniques

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

SINGLE_GROUP

All subjects will experienced the same number of buccal and saliva samples collected. This means all subjects will participate in Arm 1 and have materials collected at the same time in the event they are enrolled in arm 2.

Based on enrollment numbers and their immediate genotype, it will be determined at that time if the subject will participate in the second arm of the study.
Primary Study Purpose

SCREENING

Blinding Strategy

DOUBLE

Participants Investigators
Bi-directional sequencing results and Spartan results will not be shared with participants. The investigator will not see the bi-directional sequencing results.

Study Groups

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Sample tested in less than one-hour

Buccal samples will be collected and analyzed on the Spartan Cube CYP2C19 System immediately (\<1hr). The Spartan Cube CYP2C19 results will be compared with bi-directional sequencing results generated by a third part from a saliva sample collected from the same subject.

Group Type OTHER

Spartan Cube CYP2C19 System

Intervention Type DEVICE

Using buccal material, determine the genotype of the \*2, \*3 and \*17 SNPs of the CYP2C19 gene on the Spartan Cube CYP2C19 System.

Samples tested greater than 24 hours

Buccal samples will be collected, stored and then analyzed on the Spartan Cube CYP2C19 System greater than 24 hours after collection. The Spartan Cube CYP2C19 results will be compared with bi-directional sequencing results generated by a third part from a saliva sample collected from the same subject.

Group Type OTHER

Spartan Cube CYP2C19 System

Intervention Type DEVICE

Using buccal material, determine the genotype of the \*2, \*3 and \*17 SNPs of the CYP2C19 gene on the Spartan Cube CYP2C19 System.

Interventions

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Spartan Cube CYP2C19 System

Using buccal material, determine the genotype of the \*2, \*3 and \*17 SNPs of the CYP2C19 gene on the Spartan Cube CYP2C19 System.

Intervention Type DEVICE

Eligibility Criteria

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Inclusion Criteria

* Participants who will provide buccal samples and a saliva sample who have not eaten drank or smoked in the past 30 minutes.

Exclusion Criteria

* Participants who have eaten drank or smoked in the past 30 minutes prior to collection.
Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Mount Sinai Services

UNKNOWN

Sponsor Role collaborator

Spartan Bioscience Inc.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Shaimaa Ahmed, PhD

Role: PRINCIPAL_INVESTIGATOR

Spartan Bioscience Inc.

Locations

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Mount Sinai Services

Toronto, Ontario, Canada

Site Status

Countries

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Canada

Other Identifiers

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VNV-00229

Identifier Type: -

Identifier Source: org_study_id

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