Multi-4SCAR-T Therapy Targeting Breast Cancer

NCT ID: NCT04430595

Last Updated: 2020-06-12

Basic Information

• Recruitment Status: UNKNOWN
• Clinical Phase: PHASE1/PHASE2
• Total Enrollment: 100 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2020-06-01
• Study Completion Date: 2023-12-31

Brief Summary

The purpose of this study is to assess the feasibility, safety and efficacy of multiple 4th generation CAR-T cells targeting Her2, GD2, and CD44v6 surface antigen in breast cancer. Another goal of the study is to learn more about the activities of the multi-CAR T cells and their persistency in the patients.

Detailed Description

Breast cancer is one of the most frequent cancer types in women. The average risk of a woman in the United States developing breast cancer in her life is about 13%. That means that there is a 1 in 8 chance she will develop breast cancer. Human epidermal growth factor receptor-2 (HER2) is one of the more well-researched genes in breast cancer so far. It has been reported that HER2 gene is overexpressed in 20% to 30% of breast cancer patients. HER2 is an important target for tumor gene therapy. In 2003, scientists confirmed the existence of breast cancer stem cells. In 2012, ganglioside GD2 was confirmed as an emerging marker of breast cancer stem cells. Targeting therapies for GD2 may help improve the survival rate and cure rate of breast cancer patients. Invasion and metastasis of tumor cells is the main cause of cancer death. CD44v6 is an adhesion molecule on the cell surface, which not only promotes epithelial-mesenchymal transition, degradation and remodeling of extracellular matrix, but also participates in organ-specific metastasis of tumor cells. Studies have shown that overexpression of CD44v6 is an important factor for the invasion and metastasis of breast cancer, and is closely related to the tumor size of the breast cancer, tumor staging, and lymph node metastasis. Therefore, CD44v6 may be an important target for the treatment of breast cancer. Using Her2-, GD2- and CD44v6-specific CAR-T cells may effectively improve the immunotherapy treatment, prevent tumor cells from escaping treatment, and achieve the effect of long-term disease relief.

Conditions

Breast Cancer

Study Design

• Allocation Method: NA
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: TREATMENT
• Blinding Strategy: NONE

Study Groups

Multiple 4SCAR T cells to treat breast cancer

Multiple 4SCAR T cells to treat breast cancer

Group Type: EXPERIMENTAL

4SCAR T cells

Intervention Type: BIOLOGICAL

Infusion of 4SCAR-GD2, -Her2 and -CD44v6 CAR-T cells

Interventions

4SCAR T cells

Infusion of 4SCAR-GD2, -Her2 and -CD44v6 CAR-T cells

Intervention Type: BIOLOGICAL

Eligibility Criteria

Inclusion Criteria

1. Patients with stage III, IV or relapsed breast cancer confirmed by histology and biopsy.

2. Age: ≥ 18 years and ≤ 75 years.

3. 2 weeks at least since last chemotherapy or radiotherapy and 2 weeks at least since last systemic steroid hormone and other immunosuppressive therapy.

4. Side effects of chemotherapy have subsided.

5. The target antigens GD2, CD44v6, or Her2 is expressed in malignancy tissues by immuno-histochemical or flow cytometry.

6. Eastern Cooperative Oncology Group (ECOG) PS of 0 or 1.

7. Expected survival ≥ 12 weeks.

8. Initial hematopoietic reconstitution with neutrophils (ANC) ≥ 1×10^6/L; platelet (PLT) ≥ 1×10^8/L.

9. Proper renal and hepatic functions (ULN denotes "upper limit of normal range") with serum creatinine ≤ 2×ULN; serum bilirubin ≤ 3×ULN; AST/ALT ≤ 2.5×ULN.

10. Oxygen saturation ≥ 90%.

11. Written, informed consent obtained prior to any study-specific procedures.

Exclusion Criteria

1. Airway obstruction caused by tumor.

2. History of epilepsy or other central nervous system diseases.

3. Patients who require systemic corticosteroid or other immunosuppressive therapy.

4. History of prolonged or serious heart disease during QT.

5. Current or recent treatment (within the 28-day period prior to Day 0) with another investigational drug or previous participation in this study.

6. Inadequate liver and renal function with serum creatinine > 1.5 mg/dl; serum (total) bilirubin > 2.0 mg/dl; AST & ALT > 3 x ULN.

7. Pregnant or lactating females.

8. Serious active infection during screening.

9. Active HIV, hepatitis B virus (HBV), hepatitis C virus (HCV) infection or uncontrolled infection.

10. Patients, in the opinion of investigators, may not be eligible or not able to comply with the study.

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 75 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

The Seventh Affiliated Hospital of Sun Yat-sen University

OTHER

Sponsor Role: collaborator

Shenzhen Geno-Immune Medical Institute

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Locations

The Seventh Affilliated Hospital, Sun Yat-Sen University

Shenzhen, Guangdong, China

Site Status: RECRUITING

Countries

China

Central Contacts

Lung-Ji Chang, PhD

Role: CONTACT

c@szgimi.org

+86(755)8672 5195

Facility Contacts

Bo Wang, MD

Role: primary

wangb68377@sina.com

86-0755-23242570

Other Identifiers

GIMI-IRB-20005

Identifier Type: -

Identifier Source: org_study_id