OVSTAR TIL Trial (OVarian Cancer Co-STimulatory Antigen Receptor TIL Trial)

NCT ID: NCT04389229

Last Updated: 2020-12-16

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

WITHDRAWN

Clinical Phase

PHASE1/PHASE2

Study Classification

INTERVENTIONAL

Study Start Date

2020-07-01

Study Completion Date

2025-07-31

Brief Summary

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An open label, multi-centre Phase 1/2a study of modified and unmodified autologous Tumour Infiltrating Lymphocytes (TIL) in patients with platinum-resistant ovarian cancer. The purpose of this phase I/II study is to evaluate the feasibility and safety of both standard unmodified TIL (UTIL-01) and TIL engineered to express the co-stimulatory receptor CoStAR (CoTIL-01) in platinum resistant ovarian cancer.

Detailed Description

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This is a single-arm Phase 1/2a study of unmodified (UTIL-01) and gene modified (CoTIL-01) adoptive TIL therapy which will enrol sequentially. A total of 8 patients will be recruited to the UTIL-01 cohort to receive autologous standard unmodified TIL (phase 2). Up to 14 patients will receive autologous gene engineered TIL(CoTIL-1) in a dose escalation design (Phase 1/2a). Once patients have met all the pre-screening inclusion criteria, and that sponsor has confirmed a successful TIL harvest, a request to manufacture will be sent to the Sponsor to initiate TIL production. Manufacturing and quality control assessment is anticipated to take approximately 6 weeks. During this time, patients may receive standard of care chemotherapy (bridging chemotherapy) as deemed appropriate by the treating oncology team. Patients will proceed to the main trial after completion of bridging chemotherapy. Once the TIL product is certified for release, and that patient has consented to the main trial and has completed main trial screening assessments, study treatment can be scheduled.

Patients will receive non-myeloablative lymphodepleting pre-conditioning chemotherapy with cyclophosphamide 600mg/m2/day and fludarabine 30mg/m2/day on Day -5, -4 and -3. Chemotherapy will aim to be delivered as an outpatient, but patients can be admitted if clinically needed. Patients will be required to maintain oral hydration of \>2 litres per day. If this is felt to be difficult to achieve then the patient will be admitted for IV fluids. Patients will be admitted for TIL infusion on Day 0. The TIL infusion will be administered at least 36 hours after last dose of chemotherapy. The cells will only be thawed once an Investigator has made a positive decision to go ahead with infusion and confirmed this in writing. TIL infusion may be delayed for up to 7 days for clinical reasons or for issues regarding the cell specification. This decision must be made before final preparation for infusion. Following TIL infusion, patients will commence subcutaneous interleukin-2 at a fixed dose of 18 million units once a day. Patients must remain an inpatient for the duration of IL-2 treatment for a minimum of 7 days post TIL infusion. Recruitment to the study will occur over approximately 24-month period. Recruitment to CoTIL-01 will commence after UTIL-01. Patients will be followed up in the study for 24 months post TIL infusion.

Conditions

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Ovarian Cancer Metastatic

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

SEQUENTIAL

This study has two cohorts which will open sequentially. This is not a randomized controlled trial.
Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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UTIL-01

Single dose autologous unmodified tumour infiltrating lymphocytes

Group Type EXPERIMENTAL

cyclophosphamide

Intervention Type DRUG

600mg/m2/day for 3 days ( on day -5, -4, -3)

fludarabine

Intervention Type DRUG

30mg/m2/day for 3 days ( on day -5, -4, -3)

IL-2 (Proleukin)

Intervention Type DRUG

Subcutaneous injections at a fixed dose of 18 million units once a day following TIL infusion

Unmodified Tumour Infiltrating Lymphocytes (UTIL-01)

Intervention Type GENETIC

Single dose at 5 x 10\^9 - 5 x 10\^10

CoTIL-01

Single dose autologous gene modified tumour infiltrating lymphocytes

Group Type EXPERIMENTAL

cyclophosphamide

Intervention Type DRUG

600mg/m2/day for 3 days ( on day -5, -4, -3)

fludarabine

Intervention Type DRUG

30mg/m2/day for 3 days ( on day -5, -4, -3)

IL-2 (Proleukin)

Intervention Type DRUG

Subcutaneous injections at a fixed dose of 18 million units once a day following TIL infusion

Gene modified Tumour Infiltrating Lymphocytes (CoTIL-01)

Intervention Type GENETIC

Single dose at 2 x10\^9 (+/- 20%) (engineered TIL)

Interventions

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cyclophosphamide

600mg/m2/day for 3 days ( on day -5, -4, -3)

Intervention Type DRUG

fludarabine

30mg/m2/day for 3 days ( on day -5, -4, -3)

Intervention Type DRUG

IL-2 (Proleukin)

Subcutaneous injections at a fixed dose of 18 million units once a day following TIL infusion

Intervention Type DRUG

Unmodified Tumour Infiltrating Lymphocytes (UTIL-01)

Single dose at 5 x 10\^9 - 5 x 10\^10

Intervention Type GENETIC

Gene modified Tumour Infiltrating Lymphocytes (CoTIL-01)

Single dose at 2 x10\^9 (+/- 20%) (engineered TIL)

Intervention Type GENETIC

Other Intervention Names

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TIL engineeredTIL

Eligibility Criteria

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Inclusion Criteria

* Criteria for Pre-Screening Phase

Patients are eligible to be included in the pre-screening phase of the study only if all of the following criteria apply:

1. Women with histologically confirmed recurrent metastatic platinum-resistant high-grade serous ovarian cancer (HGSOC) (platinum resistant defined as progressing within 6 months of last platinum-containing combination chemotherapy. Patients must have received at least 1 line of prior platinum-containing combination chemotherapy and have completed at least 4 cycles of this treatment).
2. Expected to fulfil all entry criteria for OVSTAR Main Study
3. Written informed consent to Pre-Screening
4. Measurable disease by Response Evaluation Criteria in Solid Tumours 1.1
5. Have disease suitable for fresh TIL harvesting from tumour biopsies (only applicable to patients who are not participants of Sponsor's Tumour Collection Programme, PRIME)
6. Medically suitable for a general anaesthetic and surgical biopsy (only applicable to patients who are not participants of Sponsor's Tumour Collection Programme, PRIME)
7. Women of child bearing potential must be willing to practise a highly effective method of birth control once consented to pre-screening
8. World Health Organisation (WHO) Performance Status of 0 or 1 (Appendix 3)
9. Age equal to or greater than 18 years
10. Life expectancy \> 6 months
11. Absence of any psychological, familial, sociological or geographical condition potentially hampering compliance with the Pre-Screening or Main Study protocol and follow-up schedule; those conditions should be discussed with the patient before registration in the trial
12. Seronegative for HIV antibody, Hep B antigen, Hep C antibody and syphilis
13. Haematological and biochemical indices


1. Women with metastatic platinum resistant high-grade serous ovarian cancer (HGSOC) who have recurrent disease (platinum resistant defined as progressing within 6 months of last platinum-containing combination chemotherapy. patients must have received at least 1 line of prior platinum-containing combination chemotherapy and have completed at least 4 cycles of this treatment).
2. Informed consent to Main Study
3. Confirmation from Sponsor of successful TIL growth
4. Measurable disease (by Response Evaluation Criteria in Solid Tumors 1.1) on CT within 4 weeks of main study entry
5. Left ventricular ejection fraction \>50% on Echocardiogram scan
6. Patients must be willing to practice a highly effective method of birth control during treatment and for four months after receiving the preparative regime if appropriate.
7. World Health Organisation (WHO) Performance Status of 0 or 1 (Appendix 3)
8. Age equal to or greater than 18 years
9. Life expectancy \> 6 months
10. Absence of any psychological, familial, sociological or geographical condition potentially hampering compliance with the Pre-Screening or Main Study protocol and follow-up schedule; those conditions should be discussed with the patient before registration in the trial
11. Seronegative for HIV antibody, Hep B antigen, Hep C antibody and syphilis
12. Haematological and biochemical indices

Exclusion for Main Study

1. Patients receiving day 1 of their last cycle of chemotherapy or targeted therapy less than four weeks prior to pre-conditioning chemotherapy.
2. Patients receiving systemic immunosuppressive therapy including steroids at doses higher than 10mg/day of prednisolone (or equivalent) within four weeks of commencing pre-conditioning chemotherapy (unless this is required briefly as anti-emetic prophylaxis for the treatments detailed in above point 1). Patients who require such therapies intermittently due to pre-existing disorders are also excluded.
3. Patients who have any malignant or likely malignant Central Nervous System (CNS) lesion visible on CT.
4. Evidence of any active significant infection.
5. Evidence of clinically significant immunosuppression such as primary immunodeficiency (e.g. severe combined immunodeficiency disease).
6. Clinically significant cardiac disease. Examples would include unstable coronary artery disease, myocardial infarction within 6 months or class III or IV American Heart Association criteria for heart disease.
7. Patients who are at high medical risk because of non-malignant systemic disease including those with uncontrolled cardiac or respiratory disease, or other serious medical or psychiatric disorders which, in the lead clinician's opinion, would not make the patient a good candidate for adoptive TIL therapy.
8. Severe and active autoimmune disease.
9. Receiving concomitant treatment with any other experimental drugs within 4 weeks of pre-conditioning chemotherapy. Patients receiving experimental immunotherapies will be discussed with the sponsor.
10. Patients not considered likely to comply with required follow up.
11. Patients with severe allergies, history of anaphylaxis or known allergies to the administered drugs.
12. Patients who are pregnant or breast feeding should be excluded from entering the study
13. Patients who have received any prior adoptive cell therapy or organ transplant (including stem cells).
14. Caution should be exercised for patients requiring regular drainage of ascites or pleural effusions. When there is sufficient fluid to be safely drained, drainage must be performed prior to trial enrolment and pre-conditioning chemotherapy in those patients.
15. Patients with any contraindications to any of the components of the study Non Investigational Medicinal Products (cyclophosphamide, fludarabine, Interleukin-2) will be excluded
16. Patents who have received live vaccines within 4 weeks prior to TIL therapy will be excluded.

Exclusion Criteria

* Exclusion for Pre-Screening Phase

Patients will not be invited to participate in Pre-Screening if any of the following criteria apply:

1. History of a previous malignancy at another site, unless followed for \>2 years with no sign of recurrent disease (local completely excised cutaneous basal cell, squamous cell carcinoma or in situ carcinoma will be allowed).
2. Patients receiving chemotherapy, targeted therapy, immunotherapy or systemic steroids including steroid doses \>10mg/day of prednisolone (or equivalent) during the previous four weeks prior to TIL harvesting. Patients who require such therapies intermittently due to pre-existing disorders are also excluded.
3. Evidence of any active significant infection.
4. Patients who have any malignant or likely malignant Central Nervous System (CNS) lesion visible on CT.
5. Evidence of clinically significant immunosuppression such as primary immunodeficiency (e.g. severe combined immunodeficiency disease).
6. Clinically significant cardiac disease. Examples would include unstable coronary artery disease, myocardial infarction within 6 months or class III or IV American Heart Association criteria for heart disease.
7. Patients who are at high medical risk because of non-malignant systemic disease including those with uncontrolled cardiac or respiratory disease, or other serious medical or psychiatric disorders which, in the lead clinician's opinion, would not make the patient a good candidate for adoptive TIL therapy.
8. Severe and active autoimmune disease.
9. On concomitant treatment with other experimental drugs within 4 weeks of TIL harvesting.
10. Patients not considered likely to comply with required follow up.
11. Patients with severe allergies, history of anaphylaxis or known allergies to the administered drugs.
12. Patients who have received any prior adoptive cell therapy or organ transplant (including stem cells).
13. Patients who are pregnant or breast feeding should be excluded from pre-screening
14. Patients with any contraindications to any of the components of the study Non Investigational Medicinal Products (cyclophosphamide, fludarabine, Interleukin-2) will be excluded
15. Patents who have received live vaccines within 4 weeks prior to TIL therapy will be excluded

Inclusion for Main Study
Minimum Eligible Age

18 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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Immetacyte Ltd

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Fiona Thistlethwaite, PhD, MRCP

Role: PRINCIPAL_INVESTIGATOR

The Christie Hospital

Locations

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Queens Elizabeth Hospital

Birmingham, , United Kingdom

Site Status

The Christies Hospital

Manchester, , United Kingdom

Site Status

Countries

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United Kingdom

Other Identifiers

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2019-000106-30

Identifier Type: EUDRACT_NUMBER

Identifier Source: secondary_id

IMM-2019T-01-OC

Identifier Type: -

Identifier Source: org_study_id