Pentraxin-3 in Hysterectomy Patients

NCT ID: NCT04366375

Last Updated: 2020-04-30

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

40 participants

Study Classification

OBSERVATIONAL

Study Start Date

2015-01-13

Study Completion Date

2015-12-07

Brief Summary

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Comparison of the Tissue Trauma Markers Following Total Laparoscopic Hysterectomy vs Total Abdominal Hysterectomy

Detailed Description

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Acute trauma and major surgeries activate a series of cascades to evoke systemic inflammatory response. The magnitude of the resulting inflammatory response is proportional to the severity of the tissue trauma. Abdominal or laparoscopic technique is widely used for the purpose of hysterectomy surgery. It is crucial for many aspects to compare these two techniques of hysterectomy in terms of the surgery-induced trauma.

In this study, the severity of the inflammatory response resulting from the tissue trauma due to the techniques of TLH+BSO and TAH+BSO applied in our department were compared based on the PTX-3 molecule.

Conditions

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Laparoscopic Hysterectomy Abdominal Hysterectomy

Keywords

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Pentraxin-3 tissue trauma hysterectomy neutrophil to lymphocyte ratio

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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TLH+BSO

Total Laparoscopic Hysterectomy + Bilateral Salpingo-Oophorectomy N=20

PTX-3

Intervention Type DIAGNOSTIC_TEST

Pentraxin-3 ng/mL PTX-3 levels were measured using human PTX3 ELISA Kit 96 Tests/Box (Cat. No: CK-E90303) (Hangzhou Eastbiopharm Co.,Ltd., PRC) read by a ELISA device matching with the kit. For the purpose of measurement, microplate washer RT 2600 device and Rayto microplate reader RT 2100C device were used.

TAH + BSO

Total Abdominal Hysterectomy + BSO N=20

PTX-3

Intervention Type DIAGNOSTIC_TEST

Pentraxin-3 ng/mL PTX-3 levels were measured using human PTX3 ELISA Kit 96 Tests/Box (Cat. No: CK-E90303) (Hangzhou Eastbiopharm Co.,Ltd., PRC) read by a ELISA device matching with the kit. For the purpose of measurement, microplate washer RT 2600 device and Rayto microplate reader RT 2100C device were used.

Interventions

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PTX-3

Pentraxin-3 ng/mL PTX-3 levels were measured using human PTX3 ELISA Kit 96 Tests/Box (Cat. No: CK-E90303) (Hangzhou Eastbiopharm Co.,Ltd., PRC) read by a ELISA device matching with the kit. For the purpose of measurement, microplate washer RT 2600 device and Rayto microplate reader RT 2100C device were used.

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

·TLH+BSO patients and TAH+BSO patients

Exclusion Criteria

* History of a chronic disease (coronary artery disease, diabetes mellitus, hypertension, coronary liver failures, chronic kidney disease, chronic respiratory diseases, connective tissue disorders) which may affect levels of PTX-3 and other inflammatory markers.
* Prior surgery due to malignancy.
* History of an intra-abdominal surgery, except for cesarean section.
* History of any disease which may lead to intra-abdominal adhesions (endometriosis, past pelvic infection).
Minimum Eligible Age

18 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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Muğla Sıtkı Koçman University

OTHER

Sponsor Role lead

Responsible Party

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Burak Sezgin

Principal Investigator

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Burak SEZGİN, Dr

Role: PRINCIPAL_INVESTIGATOR

Mugla Sıtkı Kocman University Faculty of Medicine

Other Identifiers

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19.03.15-58-56

Identifier Type: -

Identifier Source: org_study_id