Natural Killer Cell (CYNK-001) Infusions in Adults With COVID-19

NCT ID: NCT04365101

Last Updated: 2022-05-25

Basic Information

• Recruitment Status: UNKNOWN
• Clinical Phase: PHASE1/PHASE2
• Total Enrollment: 86 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2020-05-13
• Study Completion Date: 2022-06-30

Brief Summary

This study is a Phase 1 / 2 trial to determine the safety and efficacy of CYNK-001, an immunotherapy containing Natural Killer (NK) cells derived from human placental CD34+ cells and culture-expanded, in patients with moderate COVID-19 disease.

Conditions

Coronavirus; Coronavirus Infection; Severe Acute Respiratory Syndrome Coronavirus 2; Pneumonia; Pneumonia, Viral; Lung Diseases; Respiratory Tract Disease; Respiratory Tract Infections; Coronaviridae Infections; Nidovirales Infections; RNA Virus Infections; Virus Disease; Immunologic Disease; ARDS; Immunologic Factors; Physiological Effects of Drugs; Antiviral Agents; Anti-infective Agents; Analgesics; Antimetabolites, Antineoplastic

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: SEQUENTIAL
• Primary Study Purpose: TREATMENT
• Blinding Strategy: NONE

Study Groups

Phase I

CYNK-001 infusions on Days 1, 4, and 7

Group Type: EXPERIMENTAL

CYNK-001

Intervention Type: BIOLOGICAL

CYNK-001 is an allogeneic off the shelf cell therapy enriched for CD56+/CD3- NK cells expanded from human placental CD34+ cells.

Phase II

Randomized, open label; CYNK-001 infusions on Days 1, 4, and 7 compared to Control Group: Best Supportive Care

Group Type: ACTIVE_COMPARATOR

CYNK-001

Intervention Type: BIOLOGICAL

CYNK-001 is an allogeneic off the shelf cell therapy enriched for CD56+/CD3- NK cells expanded from human placental CD34+ cells.

Interventions

CYNK-001

CYNK-001 is an allogeneic off the shelf cell therapy enriched for CD56+/CD3- NK cells expanded from human placental CD34+ cells.

Intervention Type: BIOLOGICAL

Eligibility Criteria

Inclusion Criteria

• Patient has confirmed positivity for SARS-CoV-2 as measured by rRT-PCR or other approved test to detect SAR-CoV-2 per institutional practice.
• Patient is experiencing any symptom/clinical sign of COVID-19 illness or has a positive disease-related chest x-ray/CT scan at screening.
• Patient is ≥ 18 years of age at the time of signing the Study informed consent form (ICF).
• Patient understands and voluntarily signs the Study ICF prior to any study-related assessments/procedures are conducted.
• Patient is willing and able to adhere to the study schedule and other protocol requirements.
• SpO2 ≥ 88% on room air; oxygen is permitted as delivered by nasal cannula and/or face mask at any flow rate to achieve this SpO2. Patients must have an SpO2 ≥ 92% if on supplementary oxygen.
• Ability to be off immunosuppressive drugs for 3 days prior to infusion, unless clinically indicated. Steroids are permitted if clinically indicated and at the discretion of the treating physician. If clinically indicated, careful consideration should be taken regarding the timing and tapering of high-dose steroids.
• Female of childbearing potential (FCBP)* must not be pregnant and agree to not becoming pregnant for at least 28 days following the last infusion of CYNK-001. FCBP must agree to use an adequate method of contraception during the treatment period.

• FCBP is a female who: 1) has achieved menarche at some point, 2) has not undergone a hysterectomy or bilateral oophorectomy or 3) has not been naturally postmenopausal (amenorrhea following cancer therapy does not rule out childbearing potential) for at least 24 consecutive months (i.e., has had menses at any time in the preceding 24 consecutive months).
• Male Patients must agree to use a condom during sexual contact for at least 28 days following the last infusion of CYNK-001, even if he has undergone a successful vasectomy.

Exclusion Criteria

• Patient requires supplemental oxygen delivered by mechanical ventilation, either invasive or bilevel positive airway pressure.
• Patient admitted to Intensive Care Unit / Pulmonary Acute Care Unit designated area with severe pulmonary pneumonia, ARDS or Sepsis.
• Patient is pregnant or breastfeeding.
• Patient has a history of chronic asthma requiring ongoing medical therapy or other chronic pulmonary disease that, at the discretion of the treating physician, would contraindicate participation in this study.
• Patient has any other organ dysfunction [Common Terminology Criteria for AEs (CTCAE) Version 5.0 Grade 3] that will interfere with the administration of the therapy according to this protocol.
• Patient has inadequate organ function as defined below at time of Treatment Eligibility Period:

1. Patient has aspartate aminotransferase (AST), alanine aminotransferase (ALT), or alkaline phosphatase ≥ 5 x the upper limit of normal (ULN). (It is anticipated that the infection may impact liver.)

2. Estimated glomerular filtration rate (eGFR) < 30 mL/min/1.73 m^2 as calculated using the Modification of Diet in Renal Disease Study equation (Levey, 2006) or history of an abnormal eGFR < 60. A decline of > 15 mL/min/1.73 m^2 below normal in the past year prior to infection. (It is anticipated that the infection may impact renal function.)

3. Patient has a bilirubin level > 2 mg/dL (unless Patient has known Gilbert's Syndrome).

• Patient has a known sensitivity or allergy to treatment additives or diluent substances of dimethyl sulfoxide (DMSO), PlasmaLyte A or human serum albumin (HSA). Please refer to investigational brochure (IB).
• Patient has active autoimmune disease other than controlled connective tissue disorder or those who are not on active therapy.
• Patient is immunocompromised, has known human immunodeficiency virus (HIV) positivity, or has actively been treated with immunosuppressive products prior to being infected with SARS-CoV-2.
• Patient has known active malignancy, unless the Patient has been free of disease for > 3 years from the date of signing the ICF. Exceptions include the following noninvasive malignancies:

1. Basal cell carcinoma of the skin

2. Squamous cell carcinoma of the skin

3. Carcinoma in situ of the cervix

4. Carcinoma in situ of the breast

5. Incidental histological finding of prostate cancer (TNM stage of T1a or T1b)

• Patient must not have a history of unconsciousness or hemoptysis within 2 weeks of signing informed consent form.
• Patients must not have a history of unconsciousness or hemoptysis within 2 weeks of signing ICF.
• Patients must not have end stage liver disease and/or cirrhosis.
• Patient has any significant medical condition, laboratory abnormality, or psychiatric illness that would prevent the patient from participating in the study.
• Patient has any condition including the presence of laboratory abnormalities which places the patient at unacceptable risk if he or she were to participate in the study.
• Patient has any condition that confounds the ability to interpret data from the study.

• Minimum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Access to Advanced Health Institute (AAHI)

OTHER

Sponsor Role: collaborator

Lung Biotechnology PBC

INDUSTRY

Sponsor Role: collaborator

California Institute for Regenerative Medicine (CIRM)

OTHER

Sponsor Role: collaborator

Celularity Incorporated

INDUSTRY

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Corey Casper, MD MPH

Role: PRINCIPAL_INVESTIGATOR

Access to Advanced Health Institute (AAHI)

Locations

UC Irvine

Irvine, California, United States

UC Davis Medical Center

Sacramento, California, United States

Scripps Health

San Diego, California, United States

Hackensack University Medical Center

Hackensack, New Jersey, United States

Atlantic Health

Morristown, New Jersey, United States

Atlantic Health

Summit, New Jersey, United States

Multicare Health System

Tacoma, Washington, United States

Countries

United States

References

Levey AS, Coresh J, Greene T, Stevens LA, Zhang YL, Hendriksen S, Kusek JW, Van Lente F; Chronic Kidney Disease Epidemiology Collaboration. Using standardized serum creatinine values in the modification of diet in renal disease study equation for estimating glomerular filtration rate. Ann Intern Med. 2006 Aug 15;145(4):247-54. doi: 10.7326/0003-4819-145-4-200608150-00004.

Reference Type: BACKGROUND

PMID: 16908915 (View on PubMed)

Other Identifiers

CYNK-001-COVID-19

Identifier Type: -

Identifier Source: org_study_id