Rapid Salivary Test to Detect SARS-CoV-2 (COVID-19)

NCT ID: NCT04357327

Last Updated: 2020-11-04

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

122 participants

Study Classification

INTERVENTIONAL

Study Start Date

2020-04-16

Study Completion Date

2020-07-31

Brief Summary

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The present Diagnostic Accuracy study aims at experimentally validating the use of a rapid salivary test to detect SARS-CoV-2 infection in both symptomatic and asymptomatic individuals as a preliminary approach to a mass screening program.

The study is based on a consecutive recruitment of both patients showing symptoms probably associated with COVID-19 (i.e., cough, dyspnea, fever) and asymptomatic patients with a low risk phenotype. The expected number of recruited individuals is 100.

The experimental test is a prototype of salivary test based on the Lateral Flow Immunoassay technique and is able to detect the presence of SARS-CoV-2 in saliva, especially the Spike protein (S). The comparison is represented by the nasopharyngeal swab, the gold standard of COVID-19 diagnosis.

Patients will undergo both salivary immunoassay and nasopharyngeal swab, thus the outcome assessors are blinded, since the results of the rRT-PCR analysis require at least 6 hours before being available.

The main outcomes are sensibility and specificity of the rapid salivary test, when compared with the gold standard (nasopharyngeal swab).

Detailed Description

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Conditions

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COVID-19 SARS-CoV 2 Corona Virus Infection

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

Consecutive recruitment of both patients with COVID-19 like symptoms (i.e., cough, fever, dyspnea) and patients with low risk phenotype
Primary Study Purpose

DIAGNOSTIC

Blinding Strategy

SINGLE

Outcome Assessors
The rapid salivary test will provide results within 5-10 minutes, while the rRT-PCR performed on nasopharyngeal swab shows results only after 6 hours, thus the outcome assessor of the experimental test is blinded

Study Groups

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Symptomatic patients

Patients with symptoms associated with COVID-19, i.e., dyspnea, cough, fever, etc.

Group Type EXPERIMENTAL

rapid salivary test

Intervention Type DIAGNOSTIC_TEST

a rapid salivary test to detect SARS-CoV-2 spike protein in saliva with a lateral flow immunoassay

Asymptomatic subjects

Asymptomatic patients with low risk phenotype, that means patients with a previous negative swab, no relatives affected by COVID-19 and with reduced social interaction within the last two weeks.

Group Type ACTIVE_COMPARATOR

rapid salivary test

Intervention Type DIAGNOSTIC_TEST

a rapid salivary test to detect SARS-CoV-2 spike protein in saliva with a lateral flow immunoassay

Interventions

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rapid salivary test

a rapid salivary test to detect SARS-CoV-2 spike protein in saliva with a lateral flow immunoassay

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

People who undergo nasopharyngeal swab to confirm or exclude SARS-CoV-2 infection

Exclusion Criteria

none
Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Università degli Studi dell'Insubria

OTHER

Sponsor Role lead

Responsible Party

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Lorenzo Azzi

Assistant Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Lorenzo Azzi

Role: PRINCIPAL_INVESTIGATOR

Università degli Studi dell'Insubria

Locations

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ASST dei Sette Laghi

Varese, VA, Italy

Site Status

Countries

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Italy

Other Identifiers

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68/2020

Identifier Type: -

Identifier Source: org_study_id