Trial Outcomes & Findings for Effect of Exercise on the Human Skeletal Muscle Phosphoproteome (NCT NCT04263714)

Results Overview

Myofibrillar and intracellular enrichments of L-\[ring- 13C6\] phenylalanine will be measured. MPS will be calculated using the precursor-product equation: MPS =(\[E2b-E1b\]/\[Eic x t\]) x 100. Eb represents the enrichment of bound myofibrillar protein, Eic is the average intracellular enrichment between two biopsies, and t is the tracer incorporation time in h. As we will employ 'tracer naïve' participants (had not previously participated in a study protocol where L-\[ring- 13C6\] phenylalanine was infused), a pre-infusion blood sample will be used for the calculation of resting myofibrillar MPS. The outcomes measure will be expressed as a percentage change of MPS relative to the baseline measure. i.e. intracellular enrichment of L-\[ring- 13C6\] phenylalanine relative to the baseline (resting) period.

Recruitment status

COMPLETED

Study phase

NA

Target enrollment

16 participants

Primary outcome timeframe

Pre exercise (resting) to 3 hours post-exercise

Results posted on

2024-11-21

Participant Flow

Participant milestones

Participant milestones
Measure	Exercise All subjects will perform both aerobic and resistance exercise Exercise: Aerobic exercise and resistance exercise
Overall Study STARTED	16
Overall Study COMPLETED	16
Overall Study NOT COMPLETED	0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Race and Ethnicity were not collected from any participant.

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure	Exercise n=16 Participants All subjects will perform both aerobic and resistance exercise Exercise: Aerobic exercise and resistance exercise
Age, Customized Age	21 Years n=16 Participants
Sex: Female, Male Female	6 Participants n=16 Participants
Sex: Female, Male Male	10 Participants n=16 Participants
Muscle Protein Synthesis Cohort 1	0.041 Protein synthesis (% / hour) STANDARD_DEVIATION 0.007 • n=4 Participants • Two cohorts recruited and analyzed separately.
Muscle Protein Synthesis Cohort 2	0.027 Protein synthesis (% / hour) STANDARD_DEVIATION 0.004 • n=12 Participants • Two cohorts recruited and analyzed separately.

PRIMARY outcome

Timeframe: Pre exercise (resting) to 3 hours post-exercise

Myofibrillar and intracellular enrichments of L-\[ring- 13C6\] phenylalanine will be measured. MPS will be calculated using the precursor-product equation: MPS =(\[E2b-E1b\]/\[Eic x t\]) x 100. Eb represents the enrichment of bound myofibrillar protein, Eic is the average intracellular enrichment between two biopsies, and t is the tracer incorporation time in h. As we will employ 'tracer naïve' participants (had not previously participated in a study protocol where L-\[ring- 13C6\] phenylalanine was infused), a pre-infusion blood sample will be used for the calculation of resting myofibrillar MPS. The outcomes measure will be expressed as a percentage change of MPS relative to the baseline measure. i.e. intracellular enrichment of L-\[ring- 13C6\] phenylalanine relative to the baseline (resting) period.

Outcome measures

Outcome measures
Measure	Exercise n=12 Participants All subjects will perform both aerobic and resistance exercise Exercise: Aerobic exercise and resistance exercise
Change in Acute Muscle Protein Synthesis Sarcoplasmic Protein Synthesis	123 percent change Standard Deviation 115
Change in Acute Muscle Protein Synthesis Myofibrillar Protein Synthesis	143 percent change Standard Deviation 53

Adverse Events

Exercise

Serious events: 0 serious events

Other events: 0 other events

Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Dr. Stuart Phillips

McMaster University

Phone: 9055259140

Email: [email protected]

Results disclosure agreements

Principal investigator is a sponsor employee
Publication restrictions are in place