Thiol Disulfide Balance in Cervix Cancer

NCT ID: NCT04258553

Last Updated: 2020-04-30

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

123 participants

Study Classification

OBSERVATIONAL

Study Start Date

2018-09-01

Study Completion Date

2020-02-04

Brief Summary

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The aim of this research is to evaluate thiol-disulfide balance in cervix cancer patients

Detailed Description

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To date various oxidative stress parameters were studied in patients with cervix cancer, however dynamic thiol disulfide hemostasis has not been previously investigated.

The thiol-disulfide balance in the circulation will be measured by the automated system created by Erel \& Neselioglu (1).

References

1- Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem 2014;47:326-32. doi:10.1016/j.clinbiochem.2014.09.026.

Conditions

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Cervix Cancer Oxidative Stress

Keywords

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thiol disulfide hemostasis screening early diagnosis

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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Cervix cancer

Cervix cancer n=62

Disulphide(μmol/L)

Intervention Type DIAGNOSTIC_TEST

Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with spectrophotometry

Total Oxidant Capacity(μmolH2O2Equiv/L)

Intervention Type DIAGNOSTIC_TEST

Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with spectrophotometry

Ischemia Modified Albumin(IU/mL)

Intervention Type DIAGNOSTIC_TEST

The serum Ischemia Modified Albumin concentrations were measured as described by Bar-Or et al. (Bar-Or et al. 2000).

Healthy controls

Healthy volunteers n=61

Disulphide(μmol/L)

Intervention Type DIAGNOSTIC_TEST

Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with spectrophotometry

Total Oxidant Capacity(μmolH2O2Equiv/L)

Intervention Type DIAGNOSTIC_TEST

Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with spectrophotometry

Ischemia Modified Albumin(IU/mL)

Intervention Type DIAGNOSTIC_TEST

The serum Ischemia Modified Albumin concentrations were measured as described by Bar-Or et al. (Bar-Or et al. 2000).

Interventions

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Disulphide(μmol/L)

Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with spectrophotometry

Intervention Type DIAGNOSTIC_TEST

Total Oxidant Capacity(μmolH2O2Equiv/L)

Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with spectrophotometry

Intervention Type DIAGNOSTIC_TEST

Ischemia Modified Albumin(IU/mL)

The serum Ischemia Modified Albumin concentrations were measured as described by Bar-Or et al. (Bar-Or et al. 2000).

Intervention Type DIAGNOSTIC_TEST

Other Intervention Names

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Native thiol (μmol/L) Total thiol (μmol/L) Native thiol/totalthiol(%) Disulfide/native thiol(%) Disulfide/total thiol(%) Total Antioxidant Capacity(mmolTroloxEquiv/L) Oxidative stress Index (Arbitrary Unite)

Eligibility Criteria

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Inclusion Criteria

• Histopathologically confirmed diagnosis of cervix cancer

Exclusion Criteria

* pulmonary disease
* pulmonary hypertension
* cardiac dysfunction
* renal dysfunction
* liver disease
* chronic ischemia
* systemic inflammation
* concomitant malignancy
* patients who use vitamin A,C or E (antioxidant vitamins)
* patients who use smoke
* patients who use drink alcohol
* patients who use addictive for any drug
Minimum Eligible Age

18 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

Yes

Sponsors

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Muğla Sıtkı Koçman University

OTHER

Sponsor Role lead

Responsible Party

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Burak Sezgin

Principal Investigator

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Burak Sezgin, MD

Role: PRINCIPAL_INVESTIGATOR

Mugla Sıtkı Kocman University Faculty of Medicine

Locations

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Mugla Sıtkı Kocman University Faculty of Medicine

Muğla, , Turkey (Türkiye)

Site Status

Countries

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Turkey (Türkiye)

Other Identifiers

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19/09/2018-15-4

Identifier Type: -

Identifier Source: org_study_id